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Whitley H135 HEPA Hypoxystation

The H135 HEPA is the tallest, widest, deepest hypoxic chamber in the Whitley range. It has a usable internal volume of over 550 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc. The generous internal height facilitates easy pipetting. All cell manipulations can be performed without removing them from your required hypoxic conditions. The entire cell culture incubator chamber is a working and incubation space.

  • This workstation provides precise environmental control whilst leaving plenty of room to work, incubate and conduct analysis.
  • Precise control of oxygen, carbon dioxide and nitrogen – provides flexibility in your research and confidence in your results.
  • The Whitley Internal HEPA Filtration System with optional Enhanced Biological Containment.
  • A removable front (fitted with two patented oval ports) to facilitate the transfer of large items of equipment for use inside the chamber.
  • Innovative full colour touch screen that is Ethernet-enabled for remote access. Now you can log into your workstation when you are away from the laboratory.
  • A rapid 12 litre airlock – no risk of compromising conditions inside the chamber (and it’s flushed with nitrogen as an additional cost saving).
  • Fully automated oxygen calibration routine (patent pending) – activated via the touchscreen with no need to touch or remove the sensor - saves time, no risk of user error and ensures the accuracy of your results.
  • Options Include the Whitley Automatic Humidifier – sterile, on-demand moisture addition.

For those who want to use a Seahorse XF Analyzer in their work, there is the option of connecting the H135 HEPA to a Whitley i2 Instrument Workstation.




Specification liable to change without notice

View Specifications


Capacity (Litres) 600
Port/Airlock Capacity 12 litres / Accommodates up to 44 x 96 well plates or 84 x T25 tissue culture flasks
Airlock Size 223mm deep x 190mm high
Porthole System Manual, oval ports
Gas Supplies CO2, N2 Air
Footswitch Wireless
Auto Sleeve Gassing Available
Internal Mains Socket Available
Storage Trays Available
Spotlight / Inspection Lamp Available
Single Sample Entry Available
Data Logging Available
Airlock Cycle Time (seconds) 60
Extra Cable Glands Available
Automatic Dehumidifier Fitted as standard
Automatic Humidifier Available
Removable Front Fitted as standard
Trolley Included
Dimensions (w/d/h - mm) 1452 / 1056 / 993
Weight (lbs/kg) 386 / 175
CO2 Monitoring Fitted as standard
Vacuum Take-off Available
Temperature Range (°C) 9°C above ambient up to 45°C
Touchscreen control Yes
Remote Access Fitted as standard (network access required)
HEPA Fitted as standard




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