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Whitley H35 Hypoxystation

The H35 Hypoxystation creates hypoxic and anoxic conditions within a controlled and sustained workstation environment. It is ideal for cell and tissue culture researchers wanting to accurately control oxygen, carbon dioxide, temperature and humidity. Applications for this instrument include cancer research, neurology and cardiovascular research, as well as a many other types of cell culture work.

  • Control oxygen in 0.1% increments up to 20%; carbon dioxide in 0.1% increments up to 15%; and relative humidity up to 80% - provides flexibility in your research and confidence in your results.

  • This cell culture incubator is available with removable front to facilitate thorough cleaning and the transfer of bulk samples or larger pieces of equipment for use in the workstation.

  • Built-in rapid airlock to ensure samples can be transferred into the workstation atmosphere as soon as possible (only takes 60 seconds).

  • Colour, touchscreen control panel for ease of use and for visual display of parameters such as oxygen level, carbon dioxide level, temperature, humidity, and airlock cycle status.

  • Option: Ethernet-enabled for remote access to touch screen.

  • Available with fully automatic humidification system so you can add moisture AND maintain a sterile environment.

  • Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.

Check out our dedicated Hypoxystation website at . People looking for this product may have searched for cell culture incubator, anaerobic chamber, cell culture Workstation, hypoxic Glovebox.

An ideal solution for Seahorse Analyzer users is to purchase the H35 along with the new Whitley i2 Instrument Workstation. These two workstations can be connected via the Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

View Specifications


Capacity (Litres) 300 litres
Port/Airlock Capacity 12 litres / Accommodates up to 44 x 96 well plates or 84 x T25 culture flasks or 8 x T175 culture flasks
Porthole System Manual
Gas Supplies CO2/Air/N2
Footswitch Wireless
Auto Sleeve Gassing Available
Internal Mains Socket Available
Spotlight / Inspection Lamp Available
Single Sample Entry Available
ANO2 Conditions Monitor Not applicable
Refrigeration Not applicable
Data Logging Available
Airlock Cycle Time (seconds) 60
Extra Cable Glands Available
Microscope Available
Automatic Dehumidifier Fitted as standard
Automatic Humidifier Available
Dimensions (w/d/h - mm) 1255 / 720 / 710
Weight (lbs/kg) 231 / 105
CO2 Monitoring Fitted as standard
Vacuum Take-off Available
Temperature Range (°C) 5°C above ambient up to 45°C
Touchscreen control Yes
Remote Access Fitted as standard (network access required)





H35 Hypoxystation Installation RequirementsWhitley H35 Hypoxystation Fact SheetGas consumption in a Whitley Hypoxystation is lower than other hypoxia workstations ...Whitley Hypoxystation Range Brochure

Published Papers/Posters

Stegeman H et al. Activation of AKT by hypoxia: a potential target for hypoxic tumors of the head and neck. BMC Cancer 2012Muth M, et al. Hypoxia-induced down-regulation of microRNA-449a/b impairs control over targeted SERPINE1 (PAI-1) mRNA - a mechanism involved in SERPINE1 (PAI-1) overexpression. CORRECTION. Journal of Translational Medicine 2011, 9:24Poster: Richards R & See V. Brain Tumour Cells Can Survive and Thrive in the Hypoxic Solid Tumour MicroenvironmentWennemers M, et al TRIB3 protein denotes a poor prognosis in breast cancer patients and is associated with hypoxia sensitivity. Radio Therapy and Oncology, Vol 101, Issue 1, Oct 2011, 198-202Ortiz-Barahona A, et al. Genome-wide identification of hypoxia-inducible factor binding sites and target genes by a probabilistic model integrating transcription-profiling data and in silico binding site prediction. Nucleic Acids Research, 2010 Pescador N, et al. Hypoxia promotes glycogen accumulation through Hypoxia Inducible Factor (HIF)-medicated induction of glycogen synthase. PloS ONE, 12 March 2010Tiana M, et al. A role for insulator elements in the regulation of gene expression response to hypoxia. Nucleic Acids Research 2011, 1-12Li L, et al. Rnf8 deficiency impairs class switch recombination, spermatogenesis, and genomic integrity and predisposes for cancer. The Journal of Experimental Medicine, Vol 207, No 5, April 12, 2010, 983-997Ahmadi M, et al. Hypoxia modulates the activity of a series of clinically approved tyrosine kinase inhibitors. British Journal of Pharmacology, 23 Sep 2013van de Schootbrugge C et al, Effect of hypoxia on the expression of αB-crystallin in head and neck squamous cell carcinoma. BMC Cancer, 2014Miranda et al. A Cyclic Peptide Inhibitor of HIF‑1 Heterodimerization That Inhibits Hypoxia Signaling in Cancer Cells. JACS, June 2013Asby et al. HIF-1 promotes the expression of its a-subunit via an epigenetically regulated transactivation loop. Molecular Biosystems, July 2014Herrmann et al. Cellular memory of hypoxia elicits neuroblastoma metastasis and enables invasion by non-aggressive neighbouring cells. Oncogenesis, 2015Poster: Cojocari D et al. Autophagy promotes tolerance to hypoxia through maintenance of metabolic homeostasis. 2015Poster: Koch E et al. Hypoxia promotes stemness and poor prognosis through epigenetic regulation of DICER. 2015De Bruin et al. Genome-wide analysis reveals NRP1 as a direct HIF1a-E2F7 target in the regulation of motorneuron guidance in vivoRichards R et al. Cell Cycle Progression in Gioblastoma. Mar 2016 peerj-1755Weiss J T et al. Palladium-mediated dealkylation of N-propargyl-floxuridine as a bioorthogonal oxygen-independent prodrug strategy. 2015Burrows N et al. Hypoxia-induced nitric oxide production and tumour perfusion is inhibited by pegylated arginine deiminase. Scientific Reports 6, article no. 22950. 2016Leszczynska K B et al. Mechanisms and consequences of ATMIN repression in hypoxic conditions: roles for p53 and HIF-1. Scientific Reports 6, article no. 21698. 2016Jiang Y et al. Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality. International Journal of Radiation Oncology. 2016Sandt C et al. Use of infrared microspectroscopy to elucidate a specific chemical signature associated with hypoxia levels found in glioblastoma. Royal Society of Chemistry. Analyst. 2016. 141.870Palazon A, et al. The HIF-1α Hypoxia Response in Tumor-Infiltrating T Lymphocytes Induces Functional CD137 (4-1BB) for Immunotherapy. Cancer Discovery, June 2012Labiano S et al. Hypoxia-induced soluble CD137 in malignant cells blocks CD137L-costimulation as an immune escape mechanism. Oncoimmunology 2016, Vol5, No1 1-10Vaapil M et al. 2012. Hypoxic conditions induce a cancer-like phenotype in human breast epithelial cells. PLoS ONE 7(9):e 46543. doi:10.1371/journal.pone.0046543Timpano and Uniacke. 2016. Human Cells Cultured Under Physiological Oxygen Utilize Two Cap-binding Proteins to Recruit Distinct mRNAs for Translation. Journal of Biological Chemistry 291(20):jbc.M116.717363Taylor SE, Bagnall J, Mason D, Levy R, Fernig DG, See V. 2016 Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol. 6: 160195. B et Al. BES Poster. H35Elkashef S M et al. 2016. Polysialic acid sustains cancer cell survival and migratory capacity in a hypoxic environment. Nature Scientific Reports 2016 6:33026Campbell E J et al. A scorbate availability affects tumor transplantation-take rate and increases tumor rejection in Gulo-/-mice. Hypoxia 2016:4 41-52Mohlin S et al. PI3K-mTORC2 but not PI3K-mTORC1 regulates transcription of HIF2A/EPAS1 and vascularization in neuroblastoma. Cancer Res 2015; 75(21); 4617-28Mohlin S et al. HIF2A and IGF2 expression correlates in human neuroblastoma cells and normal immature sympathetic neuroblasts. Neoplasia 2013 Vol 15, No 3, 328-34Tan Q et al. Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors. Neoplasia (2016) 18, 347-355Li R et al. Rnf8 deficiency impairs class switch recombination, spermatogenesis, and genomic integrity and predisposes for cancer. J. Exp. Mel. Vol 207 No 5, 983-997. Doi:10.1084/jem.20092437Alam et al. 2016. HIF2α contributes to antiestrogen resistance via positive bilateralBremm A et al. 2014. Cezanne (OTUD 7 B) regulates HIF-1a homeostasis in a proteasome-independent mannerPoster: Buckley et al. 2018 Characterising the function and interaction of our novel radiosensitiser with the tumour microenvironment in Oesophageal AdenocarcinomaPoster: Cowman S, and See V. Alteration of DNA Repair Activity in Hypoxic Brain Tumour Underlies Treatment ResistanceWen J et al. 2018. Suitability of cell lines as a study model for 5-hydroxymethylcytosinePoster_Ng N et al. 2018. Investigating the transcriptional response to hypoxia


Time Lapse Microscopy in a Whitley WorkstationThe Fastest O2 Control on the Market

Application Notes

Application Note_1: Interview with Biplab DasguptaApplication Note_4: Interview with Houda BenlhabibApplication Note_3: Interview with Margaret Petroff


Single Sample Entry System Options


Gioblastoma Study with Hypoxystation

Date: 2015-06-02

We study glioblastoma (where) the hypoxic niche is the home for radiation resistant glioma stem-like cells that are the likely cause for relapse....

When an incubator won't do

Date: 2015-06-02

We perform various assays while the cells are in the workstation, to assess cell death, apoptosis, migration; there are a lot of aspects where we...

Uninterrupted, stable hypoxia

Date: 2015-06-02

The Hypoxystation allows us to keep the (trophoblast) cells at their physiological oxygen, instead of at the hyperoxia that ambient air would...

Consistency of Results with Hypoxystation

Date: 2015-06-02

Removing the cells from an incubator in order to change the media or to lyse the cells after culture exposes them to altered O2 tension and...

i2 and H35 HEPA Workstations at ICR, London

Date: 2015-04-01

Whitley i2 Instrument Workstation used in deciphering the metabolic properties of Breast Cancer The Institute of Cancer Research, London is...

H35 Hypoxystation-3

Date: 2012-08-17

Accurate control of the oxygen tension to which cells are exposed to has become an important consideration in many aspects of biomedical research....

H35 Hypoxystation-2

Date: 2012-08-01

One of our Whitley H35 Hypoxystation customers comments on his use of the workstation and explains how it is helping in his...

H35 Hypoxystation-1

Date: 2012-07-26

The Importance of Oxygen Control in Cancer Research Dr Roger Phillips, at the University of Bradford’s Institute of Cancer Therapeutics,...