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Posts Tagged ‘Seahorse Hypoxic Workstation’

Novel Modified Atmosphere Cellular Handling System Allows Housing of Seahorse Analyser under Variable Oxygen Tensions

A Seahorse Biosciences Extracellular Flux Analyser (Seahorse XF Analyser) allows researchers to measure mitochondrial respiration and glycolysis in experimental cell populations. Previously, solutions for housing such an instrument in a variable oxygen atmosphere have been limited, with many users deciding not to pursue research after deeming current solutions unsuitable for generation of appropriate data. 

To this end, Don Whitley Scientific and The University of Manchester undertook a collaborative project to develop a novel system for cellular processing and Seahorse XF Analysis within a closed environment which allows oxygen variability.

To read more on this, click here.


New Hypoxystation Videos

Hot on the heels of the Oscars, we go behind the scenes in science!

Earlier this week, we filmed the latest in our series of short videos about the research that is being conducted in our Hypoxic workstations. We went to visit Dr Ayse Latif from the Faculty of Medical and Human Sciences, University of Manchester, and Dr Michael Cross from the Department of Molecular and Clinical Pharmacology, University of Liverpool.

Both Dr Latif and Dr Cross use Whitley H35 HEPA Hypoxystations in their work, and Dr Latif also uses a Whitley i2 Instrument Workstation housing a Seahorse XFe96 Analyzer. These videos will be uploaded to our website shortly.

Dr Cross spoke about his study of endothelial cells, the long-term incubation of multicellular cardiac spheroids and ischaemia in the human heart. Dr Latif discussed her work in exploiting tumour cell metabolism in order to improve treatment for gynaecological cancer patients.

Click here to have a look at our earlier video with Dr Violaine See.



New Poster on Gynaecological Cancer Cell Metabolism

On the 1st and 2nd of December, Don Whitley Scientific’s Sales Director, Steve Robertson, will be attending the Molecular Medicine Workshop (Red Temática de investigación cooperativa en cáncer) in Seville, Spain, with Dr. Ayse Latif from the University of Manchester. Dr Latif will present a poster detailing her recent work: Interrogating gynaecological cancer cell metabolism at different oxygen tensions: Results using a novel modified atmosphere cellular handling system.

Whitley i2 Instrument Workstation

Whitley i2 Instrument Workstation

Dr Latif used a Seahorse Bioscience Extracellular Flux Analyzer in her research, and the Whitley i2 Instrument Workstation has played a significant role in her findings by allowing the Analyzer to be used in a controlled hypoxic environment.


If you would like to know more about the i2 Workstation, please contact us on +44 (0)1274 595728 or email



Whitley i2 Instrument Workstation

NEW Whitley i2 Instrument Workstation

The Whitley i2 Instrument Workstation has been developed in response to a rising number of enquiries from scientists who desire to use Seahorse Extracellular Flux (XF) Analyzers* in hypoxic conditions and were dissatisfied with the solutions available. Rather than adapting an existing chamber for this purpose, Don Whitley Scientific developed a new workstation specifically to suit the precise requirements of Seahorse Bioscience.

Extensive research and careful consideration has gone into the development of the i2, which has resulted in a modular system that includes a number of novel and unique features. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the new Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

Another unique feature is the integral incubator, which enables you to precondition cellware and incubate plates and media at 37°C under the same atmospheric conditions as the XF Analyzer.

The i2 maintains an internal temperature no higher than 28°C, excludes carbon dioxide, and provides precise oxygen control.

This workstation, the latest in a long line of well-regarded modified atmosphere workstations from Don Whitley Scientific, is equipped with a generous working area in which to conduct preparatory work and is supplied complete with removable front, bespoke trolley, internal mains sockets, and a wireless footswitch to control the patented oval ports.

Whitley i2 Instrument Workstation connected to a H35 HEPA Hypoxystation

Whitley i2 Instrument Workstation connected to a H35 HEPA Hypoxystation

The 12 litre airlock, with a cycle time of just 60 seconds, accommodates up to 44 × 96 well plates or 84 × T25 tissue culture flasks plus numerous other flasks, pipettes and laboratory consumables.

The Whitley i2 Instrument Workstation is the first in a new range of larger Whitley Workstations developed to allow customers to use a variety of items of specialised equipment under modified atmospheric conditions.

The combination of a Whitley i2 Instrument Workstation and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions.

If your research requires unsurpassed accuracy, reproducibility and reliability, then insist on a Whitley Workstation.



* Not presently suitable for the recently announced Seahorse Bioscience XF-p