DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Posts Tagged ‘HEPA Filtration’

Clostridium difficile studies can be done in a Whitley Workstation

Anaerobic Workstations; Why Bigger is Better!

Anaerobic workstations offer precise and reliable atmospheric control, more stringent anaerobic conditions and consistently low running costs. One of their greatest advantages is that culture plates can be inspected at any time without disturbing the incubation conditions. In clinical laboratories, this has been proven to increase the isolation rate of anaerobic pathogens and also provide the earliest possible indication of their presence in a patient’s sample.

Don Whitley Scientific has excelled in meeting the needs of microbiologists for over 40 years, and as our customers’ needs have evolved, so too have our products. The unique Whitley Internal HEPA Filtration System is an extremely attractive feature, particularly when used in conjunction with research applications as it ensures that all of the atmosphere inside the workstation is passed through the filter hundreds of times an hour.

Over the past few years, an increased demand for using microscopes and other instruments such as the Workstation WASP Touch under anaerobic conditions resulted in the introduction of the A135 HEPA (pictured below); currently our tallest, widest, deepest workstation with a huge internal volume of 560L. The A135 is fitted with a removable front to facilitate thorough cleaning, easy replacement of the HEPA filter, and the transfer of bulk samples and larger pieces of equipment for use in the workstation. Other features include a built-in rapid airlock, integrated gas control and automatic commissioning cycle. To read the full specification and see the extensive list of factory-fitted options, please click here.

KU Leuven in Belgium were one of the first customers to purchase an A135 HEPA, and we asked them to describe the work they are doing and how this workstation has benefited their research:

A135 HEPA

“One of the key research activities in the Raes lab involves the isolation and culturing of gut anaerobes to study their metabolic and genomic properties and, ultimately, to unlock their biotherapeutic potential in human health and disease. In order to scale up these isolation efforts, we aim to move beyond conventional approaches based on agar plating and explore the application potential of novel, miniaturized, and high-throughput technologies. For testing a recently developed millifluidics-based single-cell sorting device, we soon realized that the operational space in our current DWS A35 HEPA anaerobic workstation would be insufficient. Therefore, we brought together product specialists from millifluidics and DWS to constructively discuss technical details on required dimensions, operator access, power supply, connection to an external PC, possible interference of the workstation’s pressure and the device’s heat output as well as safety aspects. This guided us towards the purchase of the DWS A135 HEPA, which is probably one of the best options currently on the market for the anaerobic integration of medium-sized pressure-controlled devices while still offering sufficient operator space and incubation volume.”

– Geert Huys, Department of Microbiology & Immunology, Rega Institute, KU Leuven & VIB Center for Microbiology, Leuven, Belgium

For one of our UK customers, however, 560L was still not big enough. They approached us in early 2018 and asked if we could build something even bigger! Always keen to rise to a challenge… Don Whitley Scientific is pleased to announce that in early 2019 we will release the first A155 HEPA Anaerobic Workstation. This exciting new product will offer all the same advantages as the A135, but with the added benefit of over 1000L of capacity. More information on the A155 will be made available in due course.

If you would like to speak to one of our sales team about our range of anaerobic workstations, or to arrange a demonstration, please contact us here.

Whitley i2 Instrument Workstation in use

Whitley Workstations Used in Novel Research Approach to Neurodegenerative Disorders

Efficient energy production is primarily driven by oxygen and is crucial in the brain, which consumes 20% of the body’s total energy whilst weighing only 2% of the total body weight. Loss of oxygen in the brain (hypoxia) can result in brain damage, which can contribute to dementia including Alzheimer’s disease (AD) and can influence amyotrophic lateral sclerosis (ALS, commonly known as motor neuron disease) disease mechanisms. Those mechanisms are often poorly understood, however, the cellular consequence of hypoxia including disruptions to energy supply are often observed in the brains of patients with these neurodegenerative disorders. The ability to model a hypoxic environment and study the effects on AD and ALS in the laboratory has historically been limited by the lack of available technology.

The SITraN laboratory takes the gold standard approach for measuring the effect of hypoxia on energy generating pathways in cells by using an XF metabolic bioanalyser (which measures energy generation in a non-invasive manner in cells, Seahorse Bioscience/Agilent), housed within an i2 Instrument Workstation and H35 HEPA Hypoxystation. The i2 chamber has been designed specifically to house and run metabolic flux assays using the XF bioanalyser in a hypoxic environment. The H35 section of the chamber is HEPA filtered and gas, temperature and humidity controlled allowing us to perform cell manipulation on our ALS and AD cell models.

Using this novel combination of technologies – SITraN aims to measure how hypoxia affects the energy generating pathways in brain cells. Their novel approach will allow the identification of the pathways involved in the cell response to hypoxia, which lead to energy disruption in patients with AD and ALS and will allow therapeutic hypoxic markers of disease to be identified for future clinical studies.

Article provided by Dr Scott Allen, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield. 

Dr Allen will be presenting at the ALS/MND Symposium in Glasgow held from 7-9 December 2018.

Whitley Hypoxystation

The Leukemic Stem Cell Niche: Adaptation to “Hypoxia” Versus Oncogene Addiction

Hematopoietic stem cells (HSC) are responsible for constantly maintaining and replenishing the supply of new blood and immune cells. They give rise to both lymphoid and myeloid progenitor cells, which then proceed to differentiate down their respective paths to form various specialized cells such as erythrocytes, macrophages, B and T cells, to name a few. Within the body, HSCs are found to reside in extremely low oxygen environments called stem cell niches (SCN). Like all other regulated cell cycles, HSCs can lead to cancers such as leukemia and lymphoma if cell division becomes uncontrolled.

MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

Figure 2: Suppression of oncogenic proteins driving non-CML blood neoplasias in the course of cell “adaptation to hypoxia.” MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

In this paper, Cheloni et al. primarily focused on chronic myeloid leukemia (CML).

The authors hypothesized that suppression of the BCR-Abl oncogene is likely a key positive regulator of LSC survival within “hypoxic” SCNs. To analyse the various mechanisms and responses that CML cells demonstrate within SCNs, the authors studied correlations between varied oxygen and glucose concentrations with the amount of BCR-Abl produced. All tests were performed using two human CML cell lines, K562 and KCL22. Testing conditions were precisely controlled to mimic the SCN environment as close as possible. Using a Hypoxystation supplied by Don Whitely Scientific, a water-saturated atmosphere comprising of 0.1% O2, 94.9% N2 and 5% CO2 was generated and maintained.

The research conducted and documented by Cheloni et al. has provided great insight into several key regulatory mechanisms associated with leukemic stem cells as well as an explanation for their notorious reputation for having high relapse rates. Additionally, they established that the triggering of oncogene suppression associated with CML is due to severe energy restriction rather than simply the “adaptation to hypoxia.”

The Don Whitley Scientific Hypoxystation provides the user incredible flexibility as it can control oxygen down to 0.1% while providing a temperature and humidity controlled environment with ample working space. The extreme precision provided by the Hypoxystation was critical to the collection of accurate and reliable data when recreating the demanding environment that comprises SCNs. The atmosphere is constantly monitored and adjusted by the real-time feedback system to ensure accuracy and ISO class 3 clean room HEPA filtration is also available for long term cell culture applications.

Choose your atmosphere with the Hypoxystation hypoxia chamber. Accurately control O2, CO2, Temperature and Humidity.   

Hypoxystation is the only hypoxic chamber purpose built for physiological cell culture research. Specifically designed to create normoxic, hypoxic and anoxic conditions within a controlled and sustained workstation environment, this hypoxic incubator is ideal for research requiring the ability to accurately control O2, CO2, temperature and humidity. The Whitley Internal HEPA Filtration System provides a particle-free internal environment that exceeds ISO 14644 class 3 clean atmosphere.  With such accurate control and the ability to manipulate cells in situ without altering the incubation environment, research into cell biology can be performed over a comprehensive range of oxygen tensions with precision. Don Whitley Scientific offer the following range of Hypoxystations:

                                                                                   

Hypoxystation H35

Hypoxystation H45

Hypoxystation H85

Hypoxystation H135

i2 Instrument Workstation

Application possibilities for Hypoxystation are endless; it is being used for research into tumour microenvironment, hypoxia pathways and HIF signalling, in vitro modelling of in situ environments, cancer cell metastasis, angiogenesis, and many other fields where cells may benefit from a more physiological atmosphere.

 

HEPA Filtration from Don Whitley Scientific

For over forty years, Don Whitley Scientific have utilised experience and expertise to ensure products make working methods more straight forward for our laboratory customers. With more and more importance placed on clean air, HEPA filtration can provide an extra measure to ensure research carried out within Whitley Workstations is done so in an environment that achieves particulate concentrations exceeding the levels detailed in ISO 14644-1 Part 1, Table 1, (Class 3) Classification of Air Cleanliness.

 

Tests have been carried out by Don Whitley Scientific that show just how much of a difference a HEPA filtration system makes to the atmosphere of a Whitley Workstation. Don Whitley Scientific have been manufacturing products for over 40 years and the introduction of HEPA filtration was a logical step to make sure our customers have an option that falls in line with the latest laboratory trends and requirements.

The benefits of the Whitley HEPA filtration system are:

  • The Whitley system utilises an internal HEPA filter. The atmosphere is cleaned quickly and continuously because the entire internal atmospheric volume of every Whitley Workstation passes through the filter hundreds of times an hour.
  • The atmosphere passing through the filter creates a laminar flow-like effect covering around 94% of the width of the chamber.
  • The location of the Whitley HEPA filter inside the workstation ensures it cannot get wet. Therefore it cannot become saturated with moisture and rendered ineffective.

Don Whitley Scientific has worked with one of the world’s leading filter manufacturers to develop a filter specifically tailored to suit the requirements of anaerobic workstations. Adding HEPA filter technology to the workstation means creating an environment where microbiology samples and cellular cultures can grow free from the risk of contamination.

HEPA filtration was introduced as an option after decades of experience in microbiology. We are confident that our thorough approach to product development and product testing has resulted in a HEPA filtration system superior to any other systems fitted to positive pressure modified atmosphere workstations. The video below explains Whitley HEPA filtration in further detail.

 

 


Explore the Whitley HEPA Workstation range


 

The Whitley A135 HEPA Anaerobic Workstation

Don Whitley Scientific is proud to present the new Whitley A135 HEPA Anaerobic Workstation: the tallest, deepest, widest anaerobic workstation in our extensive range. It is specifically designed to maintain strict anaerobic conditions within a controlled environment.
 
The Whitley A135 HEPA has an enormous capacity of almost 600 litres, perfect for working and incubation. It will accommodate a variety of items of equipment such as microscopes, plate readers and colony pickers. (Be sure to discuss your requirements with us at the earliest opportunity).

With a volume twice that of a Whitley A35 Anaerobic Workstation, the A135 also comes fitted with a large removable front – ideal for the transfer of equipment in and out of the workstation. Other great DWS features include:

•           Anaerobic conditions monitoring system
•           Internal HEPA filtration system with enhanced biological containment
•           Rapid 12 litre airlock for sample transfer
•           7” full colour touch screen, Ethernet-enabled for remote access.

135 features

Internal dimensions (w x d x h): 1100mm x 750mm x 710mm
External dimensions (w x d x h): 1452mm x 1056mm x 993mm

Without compromising quality or precision, this innovative product allows you to take your research to new heights whilst maintaining confidence in your results.

 

For more information – email us at sales@dwscientific.co.uk or call us on +44 (0) 1274 595728

Don Whitley Scientific Exhibit at Key Cardiovascular Meeting

Don Whitley Scientific recently attended the BSCR (British Society of Cardiovascular Research) Annual Meeting, held this year at The University Of Leeds. The focus of the meeting was on “Cardiovascular Disease and Diabetes” and the event featured fantastic talks and poster presentations from renowned figures in Cardiovascular research.

The Don Whitley Scientific stand featured a H45 Hypoxystation. The H45 is a large, three port cell culture workstation, ideal for replicating physiologically relevant atmospheres through the control of oxygen, temperature and humidity. Working in a Hypoxystation means cell cultures and other samples are within a stable atmosphere that matches the specific microenvironments that exist inside the human body, meaning research results are dependable and accurate.

 

At The University of Liverpool, Dr Michael Cross is conducting cardiovascular research using a Whitley H35 HEPA Hypoxystation as he looks at Low Oxygen Drug Toxicity Testing in Cardiac Spheroids

The Whitley Hypoxystation range can be viewed here

i2 and H35 HEPA at ICR London

Whitley i2 Instrument Workstation used in deciphering the metabolic properties of Breast Cancer

The Institute of Cancer Research, London, is Europe’s largest cancer centre and one of the world’s most influential research institutes, with an outstanding record of achievement dating back more than 100 years.

Staff at the ICR with their new Whitley Workstations

ICR staff with their i2 and H35 HEPA Workstations

Cancer cells display unique alterations in their signalling and metabolic circuitry in order to fuel their growth and proliferation and/or adapt to conditions of oxygen and nutrient deprivation. The Signalling and Cancer Metabolism Team, led by Dr George Poulogiannis uses high-throughput technologies including mass spectrometry-based metabolomics, and utilizes the Seahorse XF Analyzer to measure the basal oxygen consumption and glycolysis rates in order to reveal the metabolic dependencies of breast cancer-driven alterations. We are using a Whitley i2 Instrument Workstation to study the signalling and metabolic pathways that may preserve breast cancer cell viability under hypoxia. In addition to this, we have a general interest in identifying what signalling and metabolic pathways are associated with the acquisition of microsatellite (MSI) and chromosomal instability (CIN) under oxygen deprivation conditions. Finally, we are studying the role of hypoxia in cell invasion and metastasis, oncogene-induced senescence and resistance to current treatment options.

George Poulogiannis
Team Leader
Division of Cancer Biology
Institute of Cancer Research, London

i2 Workstation Testimonial Video

In this video – the latest in our series of customer testimonials – Dr Ayse Latif, Faculty of Medical and Human Sciences, University of Manchester, discusses her current research of exploiting tumour cell energy metabolism in order to improve gynaecological cancer treatments.

Dr Latif uses both a H35 HEPA Hypoxystation and an i2 Instrument Workstation. Whilst cells are initially incubated in the H35, they are then moved into the i2 via the Whitley Transfer Tunnel without exposure to ambient conditions, where they can be studied with a Seahorse XF Analyzer.

The i2 was designed to meet the exact requirements  of Seahorse Bioscience, meaning that the combination of this workstation and a Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled conditions.

Did You Know: Whitley Workstations are Ethernet-enabled for Remote Access?

Have you ever wanted to check the status of your Whitley Workstation when you were away from the lab? Well now you can. Most of our new workstations are Ethernet-enabled so you can dial in and access the touch-screen control panel from the other side of the building, or even from the other side of the world. This allows you to check the current operating conditions of the workstation and, if activated, view any of the graphical screens. If necessary, you can then make changes to the parameters, i.e. temperature, humidity, O2 and CO2 levels from wherever, whenever!

Read more

Whitley H35 Hypoxystation

Biological Containment

Looking for extra biological containment from your workstation?

If you need additional biological containment within your HEPA filtered Workstation, there is now an option to reduce biological discharge from the chamber via the gas outlets. The Whitley Enhanced Biological Containment System involves fitting HEPA filters to the two gas outlets on a Whitley HEPA Workstation.

Laboratory tests were conducted on an H35 Hypoxystation to deliberately challenge the exhaust filters with high concentrations of aerosolized bacterial cells and quantify their discharge through the exhaust valve filters.

The results of this experiment demonstrated that the system achieved a substantial reduction in bacterial loading (better than 99.999%) within the discharged gas. A paper detailing the full methodology and results of this study can be found here.