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Posts Tagged ‘Anaerobic’

Operational Flexibility of M Series Workstations

Whitley Microaerobic Workstations from Don Whitley Scientific are designed to allow users to control oxygen, carbon dioxide and hydrogen within safe working limits.
By using sophisticated sensors, actual gas levels can be monitored on the touchscreen, giving the user confidence that their samples are being incubated for the appropriate atmosphere.

M series workstations create accurate and reproducible conditions within safe operating parameters. They provide the most flexibility of any models in the Don Whitley Scientific range as they allow Anoxic/Anaerobic, Microaerobic or Hypoxic atmospheres to be created if desired.


Microbiological Applications:
• Anaerobic atmosphere: An oxygen free atmosphere.
• Microaerobic atmosphere: An atmosphere suitable for growing organisms that require oxygen levels lower than those found in air. Hydrogen can be included in the atmosphere if required.

Cell/Tissue Culture Applications:
• Hypoxic atmosphere: An atmosphere suitable for growing cells under physiologically relevant conditions, containing oxygen levels of between 0.1% and 20%, along with carbon dioxide at the required concentration (up to 15%).

Microaerobic operation using an M series workstation
For standard operation of Whitley Microaerobic Workstations, four separate gas cylinders of carbon dioxide, oxygen free nitrogen, compressed air and a 10% hydrogen/90% nitrogen mix, need to be connected to the workstation. This allows the user to select a safe workstation atmosphere from the following available ranges:

Oxygen 0 – 20%
Carbon Dioxide 0 – 15%
Hydrogen 0 – 5%
Nitrogen Balance

Hypoxic operation using an M series workstation
For hypoxic operation only carbon dioxide, oxygen free nitrogen and compressed air should be connected to the workstation. As hydrogen is not required no gas needs to be connected to the 10% hydrogen/90% nitrogen gas input. This will allow the user to choose from the following:

Oxygen 0 – 20%
Carbon Dioxide 0 – 15%
Nitrogen Balance


Anoxic/anaerobic operation using an M series workstation
The M Series can be operated anaerobically (Anoxic conditions) by introducing a catalyst. Any oxygen then present in the workstation atmosphere combines with hydrogen to create water. The catalyst facilitates this reaction and any excess water condenses on a chilled plate and is automatically removed from the workstation.

M35 Microaerobic Workstation used in Campylobacter jejuni research 

For more information – email us at or call us on +44 (0) 1274 595728

New Whitley Hypoxystation Video Launched

Don Whitley Scientific are proud to present a new video that showcases our wide range of Whitley Hypoxic Workstations. The video also features comments from renowned researchers, explaining how a Whitley Hypoxystation benefits their research.

The video is another addition to a growing YouTube channel which features similar videos showcasing our wide product range and company values, including anaerobic and microaerobic workstations, customer testimonials, an introduction to Don Whitley Scientific and the level of service on offer for our customers.



Researchers featured in this video
Dr Karen Wright – Dr Karen Wright uses a Whitley H35 Hypoxystation in the cell culture laboratory at Lancaster University to expedite a component of her research, namely culturing human gut cells at low physiological levels of oxygen. – Testimonial in full

Dr Michael Cross – Dr Cross and his group use their H35 HEPA Hypoxystation to test cardiovascular drug toxicity in a cardiac spheroid model under physiologically relevant oxygen tensions and also periods of extreme hypoxia/ischemia. – Testimonial in full


Click here to explore the full range of Whitley Hypoxystations


Contact our sales team for more information on 01274 595728 or email For the full range of Whitley products, visit our website at

Anaerobic Conditions in Jars – When funds don’t allow for a Whitley Workstation

One of the products in the Don Whitley Scientific range that sometimes gets overlooked is the Whitley Jar Gassing System (WJGS). When your budget or number of samples are just not enough to justify the purchase of even a small workstation, such as the DG250, the WJGS could be the answer.

Who could benefit from a WJGS?

Perhaps you only have a small number of plates to incubate. Perhaps you use gas packs. Perhaps you have a large number of jars already and don’t want to invest in a different solution. The WJGS can also be interesting for those who want to incubate small numbers of plates in different gas mixtures, as the WJGS can be connected to any gas mixture you want to use.


What are the cost implications?

The system is deceptively simple but highly effective and has a number of key benefits that have proven very popular. One of the main positives is the low running costs of the WJGS as opposed to the high cost of gas generating kits. Whilst there is an obvious initial investment in the purchase of a WJGS, the operating costs will save money in the longer term.


What are the features and benefits?

In addition to the cost savings that can be achieved, the WJGS provides many other benefits, including:

  • Create the perfect anaerobic conditions in your jars in just 2 minutes and for microaerophiles, in 15 seconds. The WJGS reduces the cost of creating microaerobic conditions by 98% and for anaerobic conditions by 89%.
  • Most types of jar can be adapted for use with the WJGS so if you already have a supply of jars, check with us to find out if they are suitable or can be adapted.
  • If you are a busy clinical lab with an anaerobic workstation but also have a few microaerophiles that you need to cultivate, you could attach a WJGS to the workstation and run a microaerobic cycle for those samples. It gives you additional flexibility as well a little extra anaerobic capacity at times of high demand.
  • Having samples in jars means that you can incubate at alternative temperatures than are possible inside a workstation.
  • You can be confident that a vacuum has been drawn and that the jar is sealed and airtight. With a gas pack you have no way of knowing whether the container is leaking.
  • There is an optional printer to provide full traceability.
  • If you need to open the jar to add more samples once you have activated a gas pack, it’s a waste as you will have to activate another gas pack. However, with jars you can open them as many times as you want and re-gas them for pennies.
  • The other benefit of the ability to re-gas so cheaply is that samples don’t have to be left on the bench for hours whilst enough are assembled to justify the use of a gas
  • A further consideration is that the used gas pack needs to be dealt with as hazardous waste. It needs autoclaving and disposing of accordingly. There is no such consideration with the WJGS.

Royal Stoke Hospital recently joined the long list of Whitley Jar Gassing System customers. The new system will replace the old style gassing system the hospital has been using and will improve their working methods and reduce costs.



The video below shows how easy the system is to use


So, if your budget won’t stretch to a Whitley Workstation or you want the flexibility to incubate in jars at different temperatures, the Whitley Jar Gassing System could be the perfect solution.


For further information, please call our sales team on +44 (0)1274 595728 or email


New Video: Whitley Jar Gassing System Animation

Don Whitley Scientific have released a new video, an animation highlighting the effectiveness of using a Whitley Jar Gassing System to create anaerobic and microaerobic conditions.

The animation shows key features that make the Whitley Jar Gassing System a fast, low cost solution for creating specific atmospheric conditions whilst being a convenient and easy piece of equipment to use. Using the clear touchscreen display, the user can create anaerobic conditions within 2 minutes and microaerobic conditions in a rapid 15 second cycle. These cycles are displayed as graphs on the touchscreen and then available to print out for traceability and accreditation purposes.

The WJGS is part of a wide range of Don Whitley Scientific products that provide efficient and reliable conditions for a range of microbiology applications. When released the Whitley Jar Gassing System was an innovative new way to create anaerobic/microaerobic conditions and it has evolved with technology, continuing to provide a low cost solution to create the relevant atmosphere for working with anaerobes and microaerophiles.


Scientist Working in Whitley Workstation

Anaerobic Sample Collection and Transport – Free guide available

This free guide, entitled “Optimising Specimen Collection and Transportation for Isolation of Anaerobic Bacteria” provides advice for the clinicians you work with on the isolation and transportation of anaerobes. The publication was written by Selina Scotford of the UK Anaerobe Reference Unit.

Written for the guidance of clinicians working with anaerobic bacteria, the leaflet details key points to consider when collecting and transporting anaerobic samples. The guide gives information on the required specimen types for successful isolation  as well as vital do’s and dont’s for sample transportation.

The content of the guide is based upon a lecture Selina Scotford delivered during the 2015 Practical and Clinical Microbiology of Anaerobes course. Don Whitley Scientific proudly sponsors this course which is run by the UK Anaerobe Reference Unit (UKARU). The UKARU is located within Public Health Wales Microbiology at the University Hospital of Wales in Cardiff.

Guide for Clinicians Front Page

Request your free* copy (or multiple copies) here today

* Free to UK addresses only


Modifications made to Whitley A55 Anaerobic Workstation

Don Whitley Scientific products can be used across a wide range of scientific applications. Sometimes modifications can be made to our workstations to suit specific user needs. This happened recently when a customer required a different style of porthole for an Whitley A55 Anaerobic Workstation to allow them to work more freely with equipment inside the cabinet.

In this case we manufactured round porthole doors that can be removed and stored securely within the cabinet, thus providing maximum flexibility. This also allows more room to use the Whitley Automated Spiral Plater inside the workstation. The advantage of this being that all the customer’s sample processing is done in anaerobic conditions, meaning more consistent and reliable results.

The Whitley A55 Anaerobic Workstation provides a huge working capacity, enabling easy processing and examination of samples. With four ports (available as instant access or sleeved portholes) along the body of the workstation, two people can work side-by-side to process samples and introduce or remove samples via rapid airlocks on each side of the unit.

The A55 is one model in a wide range of anaerobic and hypoxic workstations from Don Whitley Scientific, each with unique features and options that are made with various scientific applications in mind. If you’re looking for quality equipment to benefit your results, don’t hesitate to get in touch and see what Don Whitley Scientific can provide for you.