Contact Us +44 (0) 1274 595728

Follow Don Whitley Scientific

Archive for September, 2016

World Heart Day, September 29, 2016

Today is World Heart Day, when organisations globally advocate “creating heart-healthy environments”, for example by eating healthier and exercising 30 minutes a day. Healthy environments are also a priority for Don Whitley Scientific and Hypoxygen, whose Hypoxystation is used globally by cardiovascular researchers to mimic the in vivo environment, so cells behave in a physiologically relevant manner. Temperature, CO2, oxygen and humidity are controlled in the Hypoxystation, and cells are cultured and manipulated in that physiological environment without ever experiencing the “oxygen shock” of the bench atmosphere. The in vivo environment recreated in the Hypoxystation is healthy with regard to metabolism, proliferation, signaling, and drug responsiveness, to name a few.

Hear Dr. Michael Cross, Molecular and Clinical Pharmacology Department, University of Liverpool, speak about his work with cardiac spheroids to assess cardiovascular drug toxicity. “The H35 allows us to generate oxygen levels that reflect the in vivo physiology these cells would be exposed to. We chose the Hypoxystation with its oxygen profiling feature, which allows us to recreate cycles of ischemia, where oxygen levels typically sink to 1-3%”.

On the other side of the Pacific, Dr Vaibhao Janbandhu of the Division for Developmental and Stem Cell Biology at the Victor Chang Cardiac Research Institute (VCCRI) in Sydney, uses the H35 Hypoxystation in his work with cardiac stem cells as targets for regeneration therapies.

Read more from the Hypoxygen blog here

Don Whitley Scientific at Microbe 2016

Don Whitley Scientific once again exhibited at Microbe 2016, the bi-annual conference organised by the Sheffield branch of the Institute of Biomedical Scientists. Microbe is one of the most important events in the calender for many local, national and international biomedical scientists. The symposium featured a programme of fantastic speakers on subjects such as viral infections, infectious diseases and antimicrobial resistance.

This year’s theme was “Book Titles”. Don Whitley Scientific appeared as The Addams Family with a stand dressed with cobwebs, candelabras and other spooky props. The team dressed up as the iconic family, featuring Pugsley, Wednesday, Uncle Fester, Lurch and Cousin Itt.


Exhibited at the event were the ChromaZona and DG250 Anaerobic Workstation. ChromaZona is an automated microbial identification and antibiotic susceptibility testing (AST) analysis system for faster results in busy clinical laboratories. The Whitley DG250 Workstation has a small footprint but offers a generous working space to process, culture and examine anaerobic samples. Representatives from Copan Italia were also at the Don Whitley Scientific stand to discuss WASPLab and in particular the reading station where plated samples can be scrutinised under a high level of detail.


Cancer Cell Study Carried Out At The University Of Bradford

A paper entitled “Plysialic acid sustains cancer cell survival and migratory capacity in a hypoxic environment” has been published by researchers at The University of Bradford and The University of Huddersfield. The study looks at Polysialic acid (a unique carbohydrate polymer expressed on the surface of neuronal cell adhesion molecules) and its association with tumour cell and adhesion in hypoxia. Their findings provide the first evidence that polySia expression sustains migratory capacity and is associated with tumour cell survival in hypoxia.

A key part of the study involved the use of a Whitley H35 Hypoxystation. The importance of using a piece of equipment such as a Whitley Hypoxystation is down to hypoxia having a profound effect on cancer cell growth as it occurs in poorly vascularised areas of tumours. Klaus Pors, Senior Lecturer In Chemical Biology, provided the quote below:

Dr Robert Falconer and colleagues are studying polysialyl transferases (polySTs), responsible for the biosynthesis of polysialic acid (polySia), as a potential antimetastatic therapeutic strategy. PolySia is a unique carbohydrate polymer capable of modulating cell-cell and cell-matrix adhesion, migration, invasion and metastasis in a number of cancers. In this study we have employed Don Whitley H35 Hypoxystation to analyse how polySia sustains cancer cell survival and migratory capacity in a hypoxic environment. We believe these results contribute significantly to our understanding of how polySia supports an aggressive phenotype and further studies are underway to underpin these findings in a therapeutic context.

Furthermore, the paper also states that the “results have significant potential implications for polyST inhibition as an anti-metastatic therapeutic strategy and for targeting hypoxic cancer cells”.

The group are set to continue this line of work, using the Whitley H35 Hypoxystation. Keep an eye on the DWS blog for more articles on this.


Don Whitley Scientific Exhibit at Key Cardiovascular Meeting

Don Whitley Scientific recently attended the BSCR (British Society of Cardiovascular Research) Annual Meeting, held this year at The University Of Leeds. The focus of the meeting was on “Cardiovascular Disease and Diabetes” and the event featured fantastic talks and poster presentations from renowned figures in Cardiovascular research.

The Don Whitley Scientific stand featured a H45 Hypoxystation. The H45 is a large, three port cell culture workstation, ideal for replicating physiologically relevant atmospheres through the control of oxygen, temperature and humidity. Working in a Hypoxystation means cell cultures and other samples are within a stable atmosphere that matches the specific microenvironments that exist inside the human body, meaning research results are dependable and accurate.


At The University of Liverpool, Dr Michael Cross is conducting cardiovascular research using a Whitley H35 HEPA Hypoxystation as he looks at Low Oxygen Drug Toxicity Testing in Cardiac Spheroids

The Whitley Hypoxystation range can be viewed here