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Archive for April, 2016

HypOxygen at the AACR Annual Meeting

Our American distributor, HypOxygen, are exhibiting at the American Association of Cancer Research annual meeting. Exhibiting the Whitley H35 Hypoxystation and the Whitley i2 Instrument Workstation, HypOxygen will be promoting the importance of using this equipment when working at different stages of cell culture within the context of cancer research. The words below, from HypOxygen, describe this in more detail.

The hallmarks of cancer, from reprogramming energy metabolism through sustained angiogenesis to evasion of the immune response, are linked to the low oxygen tension typical of the tumour microenvironment. Hypoxia is a crucial determinant of cellular signalling, inflammation, cancer progression, and therapy resistance, through pathways mediated by HIF’s and other oxygen-sensing factors. That’s why cancer research consistently benefits from the use of a closed workstation for cell culture that supports in vivo physiology and behaviour. Based on these insights, the Hypoxystation creates conditions that mimic the cellular microenvironment with regard to oxygen tension, temperature, and humidity, while class 3 clean air through HEPA filtration and sterile humidity guarantee worry-free long-term cell culture.

The capacity to stably incubate cell cultures at hypoxic conditions down to 0.1% oxygen depends on precise monitoring of conditions, accurate regulation of gasses, and on the ability to retain the atmosphere for a long time once it is achieved. Based on studies performed at Don Whitley Scientific recently, the “at rest” gas consumption in our H35 Hypoxystation is much lower than in other workstations: Nitrogen 3.5 l/24 hrs, CO2 1 l/24 hours. In contrast, published nitrogen usage in competitors’ hypoxia workstations is 28 and 47 times higher, and CO2 consumption is 5-7 times higher.

You can find the details of that study on the HypOxygen website here.


HypOxygen at the AACR


HypOxygen will be showing the H35 and i2 Instrument Hypoxystations for physiological cell culture at the AACR Annual Meeting in New Orleans from 17/04/16 – 20/04/15 at booth 448.

Scientist Working in Whitley Workstation

Anaerobic Sample Collection and Transport – Free guide available

This free guide, entitled “Optimising Specimen Collection and Transportation for Isolation of Anaerobic Bacteria” provides advice for the clinicians you work with on the isolation and transportation of anaerobes. The publication was written by Selina Scotford of the UK Anaerobe Reference Unit.

Written for the guidance of clinicians working with anaerobic bacteria, the leaflet details key points to consider when collecting and transporting anaerobic samples. The guide gives information on the required specimen types for successful isolation  as well as vital do’s and dont’s for sample transportation.

The content of the guide is based upon a lecture Selina Scotford delivered during the 2015 Practical and Clinical Microbiology of Anaerobes course. Don Whitley Scientific proudly sponsors this course which is run by the UK Anaerobe Reference Unit (UKARU). The UKARU is located within Public Health Wales Microbiology at the University Hospital of Wales in Cardiff.

Guide for Clinicians Front Page

Request your free* copy (or multiple copies) here today

* Free to UK addresses only


Did You Know: You need to use the appropriate autoclave cycle for different load types?

Unlike their medical counterparts, laboratory autoclaves need to be capable of running a variety of different sterilisation cycles, for liquids, instruments and waste. 

Each of these cycles has unique requirements and the unit should be equipped to meet these varying needs. Cycles should be specific to the load type with parameters designed to meet proper sterilisation. An example of these required cycles would be when it comes to autoclaving hollow items such as tubing sets, which are frequently used but often inadequately sterilised. For such items, cycles need to be used that use a prefractionated vacuum that eliminates air pockets and maximises steam penetration.

During liquid sterilisation, most autoclaves reach a certain temperature measured inside of the vessel that corresponds to the steam inside the chamber, for a certain length of time, without ever measuring the specific temperature of the liquids to be sterilised.  Many laboratories would be surprised to find that their liquids never reach sterilisation temperature at any point during the sterilisation time even though the autoclave chamber temperature has reached 121 degrees Celsius.

To ensure liquids reach sterilisation temperature, Tuttnauer autoclaves employ the use of a flexible PT 100 temperature probe. The PT 100 extends down into the sterilisation chamber and measures the temperature in a reference vessel; the autoclave’s temperature and run time are based on this liquid temperature, not the temperature of the sterilisation chamber.

Don Whitley Scientific is accredited by UKAS to provide calibration and validation services for heat sterilisation equipment, temperature controlled processes and temperature indicators. This service is vital to make sure autoclaves are running efficiently and appropriately, and this level of after sales support sets Don Whitley Scientific apart from other autoclave suppliers.

Learn more about Tuttnauer Laboratory autoclaves

This is another article in the ‘Did you know’ series, introducing facts you may not know about products and services supplied by Don Whitley Scientific.