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Archive for March, 2016

Modifications made to Whitley A55 Anaerobic Workstation

Don Whitley Scientific products can be used across a wide range of scientific applications. Sometimes modifications can be made to our workstations to suit specific user needs. This happened recently when a customer required a different style of porthole for an Whitley A55 Anaerobic Workstation to allow them to work more freely with equipment inside the cabinet.

In this case we manufactured round porthole doors that can be removed and stored securely within the cabinet, thus providing maximum flexibility. This also allows more room to use the Whitley Automated Spiral Plater inside the workstation. The advantage of this being that all the customer’s sample processing is done in anaerobic conditions, meaning more consistent and reliable results.

The Whitley A55 Anaerobic Workstation provides a huge working capacity, enabling easy processing and examination of samples. With four ports (available as instant access or sleeved portholes) along the body of the workstation, two people can work side-by-side to process samples and introduce or remove samples via rapid airlocks on each side of the unit.

The A55 is one model in a wide range of anaerobic and hypoxic workstations from Don Whitley Scientific, each with unique features and options that are made with various scientific applications in mind. If you’re looking for quality equipment to benefit your results, don’t hesitate to get in touch and see what Don Whitley Scientific can provide for you.



When pre-mixed gas is hard to source – you need a three gas workstation

Recent reports suggest that in some parts of the world pre-mixed gas is expensive and becoming ever more difficult to source. Whitley TG Anaerobic Workstations are ideal in such circumstances as they run from three separate cylinders: nitrogen, hydrogen and carbon dioxide. These three gas workstations, which are unique to Don Whitley Scientific, can operate at around 30% cheaper than the dual gas version, making them extremely cost-effective.

The Whitley A85TG and A95TG Workstations have large, 30 litre airlocks that ensure the minimum amount of atmospheric oxygen enters the chamber when samples are transferred. The A95TG is the larger of the two and features 4 portholes, which enables two people to work in the cabinet at the same time.

Conditions inside the workstation can be monitored with the Whitley Anaerobic Conditions Monitor. This fully integrated option provides confirmation that anaerobic conditions exist inside the workstation and gives an early indication if conditions begin to vary. A factory-fitted only option, this system displays real time oxygen levels on the touch screen display using coloured segments – green, amber or red – depending upon the level of oxygen present. Recorded data can be downloaded to a memory stick in a few seconds for further analysis. This option comes complete with data logging. Other features include internal power sockets, bespoke trolley and single plate entry systems.

Whitley H35 Hypoxystation

TRACER mimics oxygen and nutrient gradients in tumours

Our US/Canada distributor, HypOxygen, wanted to find out more about Whitley H35 and H45 Hypoxystation customer Brad Wouters’ recent research, especially the newly published paper describing his three-dimensional tumour TRACER project, published as “A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients“ (Rodenhizer et al., Nature Materials 15, 227–234, 2016).

Interview with Brad Wouters, Princess Margaret Cancer Center, Toronto.  March 4, 2016

“The paper describes a new device that enables us to create naturally occurring oxygen gradients, such as the ones found in tumours. The TRACER (engineered tumour roll for analysis of cellular environment and response) developed by Alison McGuigan of the University of Toronto is basically an engineered tumour that is assembled by rolling a single-component biocomposite sheet. Respiration-induced oxygen and metabolite gradients are established inside the device, so it’s an alternative to other 3D models such as spheroids. The benefit is that the TRACER rolls can be rapidly disassembled, enabling you to interrogate the biological phenotypes or properties, characteristics, and metabolites of the cells in those defined locations within the gradient, in the six layers of the tracer. The cells can be removed in a variety of ways; you can remove the cells in a viable way and process them as you would any cells, to do flow cytometry, metabolomics, cell survival, proliferation.

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Liverpool Mini-Exhibition a success for DWS

Representatives from Don Whitley Scientific recently held a mini exhibition with their demonstration vehicle at the University of Liverpool’s Biosciences Building. Various members of university staff and students made their way to the vehicle to get a first hand look at Don Whitley Scientific equipment.

With the vehicle, Don Whitley Scientific can take a range of equipment to any site, presenting a comfortable and professional environment to discuss what DWS products may be suitable for a customer’s work. On this occasion the products on show were a H35 Hypoxystation and the DG250 Anaerobic Workstation. Customers could also learn about other Whitley Workstations and our Ebers products, such as the TC-3 Bioreactor, which is designed to be used for creating cell culture experimental set-ups with user defined mechanical loading profiles.

During their time in the vehicle, guests were also treated to a coffee and a Danish pastry. It turned out to be a very worthwhile visit to the truck for Helen Wright, a research fellow in the Department of Biochemistry, who won the prize draw and took home a Nespresso coffee machine.

A visit from the Don Whitley Scientific demonstration vehicle may be a great way to save your time whilst ensuring you and your staff are kept up-to-date with the latest technology. If you think this could be suitable for your company or university, please get in touch and we can look at arranging a visit to your site. Email for more information.


Thuy Do Video Testimonial – Whitley A45 Workstation

In this video testimonial, Dr Thuy Do from the University of Leeds explains how her research benefits significantly from the use of a Whitley A45 Workstation.

Thuy Do’s research focuses on the oral microbiota and its links with various infectious diseases, as well as looking at complex interactions within oral biofilms.  Her current work uses high throughput sequencing to identify biomarkers for health or disease. By using a Whitley Workstation she can analyse her sample throughput under the necessary physiologically relevant conditions.

If you are working with any equipment from Don Whitley Scientific and would like to let us know about your work and your overall experience with our products, please don’t hesitate to get in touch via our contact formFacebook or Twitter. We now feature a variety of customer testimonials on our product pages and these can be accessed through the “testimonials” tab (where we have a testimonial for that product).

Whitley Hypoxystations used in Oxygen study

Two Whitley Hypoxystations were used in a study by the Ontario Institute for Cancer Research.

The paper, entitled “Two phases of disulfide bond formation have differing requirements for oxygen” details how H35 and H85 Hypoxystations were used at various points of the experiment to assess and monitor how cancer cell lines react differently to hypoxic conditions.

Brad Wouters, who contributed to the study, previously suggested that for this type of work a Hypoxystation is the only option to maintain a consistent hypoxic environment. He also went on to state that Whitley Hypoxystations contribute significantly in his work towards cancer therapies, “The continuous hypoxia we achieve in the workstation is a prerequisite for studies with hypoxia-activated drugs used in cancer therapy”.

Both H35 and H85 Hypoxystations offer the best solution when it comes to incubating and working with samples at low oxygen conditions. The rapid airlock system means there is no risk of compromising the atmosphere created within the cabinet itself, enabling dependable, reliable and consistent results.

You can read the full paper by Marianne Koritzinsky and her peers by clicking here.