Whitley i2 Instrument Workstation used in deciphering the metabolic properties of Breast Cancer The Institute of Cancer Research, London is...
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Whitley i2 Instrument Workstation
The Whitley i2 Instrument Workstation has been developed in response to a rising number of enquiries from scientists who desire to use Seahorse Extracellular Flux (XF) Analyzers* in hypoxic conditions and were dissatisfied with the solutions available. Rather than adapting an existing tissue culture incubator for this purpose, Don Whitley Scientific developed a new tissue culture incubator specifically to suit the precise requirements of Seahorse Bioscience.
Extensive research and careful consideration has gone into the development of the i2, which has resulted in a modular system that includes a number of novel and unique features. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the new Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.
Another unique feature is the integral incubator, which enables you to precondition cellware and incubate plates and media at 37°C under the same atmospheric conditions as the XF Analyzer.
- The i2 maintains an internal temperature no higher than 28°C, excludes carbon dioxide, and provides precise oxygen control.
- This workstation, the latest in a long line of well-regarded modified atmosphere workstations from Don Whitley Scientific, is equipped with a generous working area in which to conduct preparatory work and is supplied complete with removable front, internal mains sockets, and a wireless footswitch to control the patented oval ports.
- The 12 litre airlock, with a cycle time of just 60 seconds, accommodates up to 44 × 96 well plates or 84 × T25 tissue culture flasks plus numerous other flasks, pipettes and laboratory consumables.
- The Whitley i2 Instrument Workstation is the first in a new range of larger Whitley Workstations developed to allow customers to use a variety of items of specialised equipment under modified atmospheric conditions.
- The combination of a Whitley i2 Instrument Workstation and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions.
If your research requires unsurpassed accuracy, reproducibility and reliability, then insist on a Whitley Workstation.
* Not presently suitable for the Seahorse Bioscience XF-p
|Port/Airlock Capacity||12 litres / Accommodates up to 44 x 96 well plates or 84 x T25 tissue culture flasks|
|Airlock Size||223mm deep x 190mm high|
|Porthole System||Manual, oval ports|
|Gas Supplies||Air and Nitrogen|
|Auto Sleeve Gassing||Available|
|Internal Mains Socket||Fitted as standard|
|Spotlight / Inspection Lamp||Available|
|Single Sample Entry||Available (front loading only)|
|Airlock Cycle Time (seconds)||60|
|Extra Cable Glands||Available|
|Removable Front||Fitted as standard|
|Dimensions (w/d/h - mm)||1702 / 825 / 928|
|Weight (lbs/kg)||286 / 130|
|CO2 Monitoring||Not Applicable|
|Temperature Range (°C)||<28°C (Main Chamber)|
LiteratureWhitley i2 Instrument Workstation - Installation DocumentWhitley i2 Instrument Workstation Fact SheetWhitley i2 Instrument Workstation BrochureHypoxystation Range Brochure
Published Papers/PostersLatif A, et al. Interrogating gynaecological cancer cell metabolism at different oxygen tensions: Results using a novel modified atmosphere cellular handling system. Dec 2014Latif A, et al. Interrogating gynaecological cancer cell metabolism at different oxygen tensions reveals simvastatin as metabolic regulator.May 2015
Application NotesApplication Note: Novel Modified Atmosphere Cellular Handling System Allows Housing of Seahorse Analyzer under Variable Oxygen Tensions
i2 and H35 HEPA Workstations at ICR, London