DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


Contact Us +44 (0) 1274 595728 sales@dwscientific.co.uk

Follow Don Whitley Scientific

Posts Tagged ‘hypoxic workstation’

Distributor Meeting Held at New Factory

Last week, DWS welcomed 38 representatives from over 20 different countries for the first International Distributor Meeting to be held at our new factory in Bingley. On the first night we held a welcome dinner, and as part of the evening there was a presentation to four of our distributors who had excelled in certain areas. Our special thanks and congratulations to VWR (Sweden), Lab Technologies (India), Cold Spring Biotech Corp (Taiwan) and Hua Yue Enterprise Holdings Ltd (China). Another award should go to our colleagues from AS1 Limited in New Zealand, who travelled over 11,000 miles to be with us!

It was a busy few days packed with sales and installation training on the WASP Touch and our hypoxic, anaerobic and microaerobic workstations. The meeting provided an invaluable opportunity for the distributors to get hands-on experience with our whole product range and to receive training from the people who design and build our equipment on a daily basis. One of the attendees commented: “Our thanks to you and all your colleagues for a brilliant couple of days and a superbly structured meeting which I’m sure will be very fruitful.”

As with our past meetings, we felt it was important to balance out the hard work with some fun and offer our guests a chance to socialise after the training sessions. Our evening entertainment included lots of delicious food and activities such as bowling, cheese-tasting, a magic show (courtesy of our wonderfully multi-talented sales rep, Stuart) and a musical performance from a local brass band.

Distributors_Oct18_Group PhotoWe are very grateful to the distributors who took time out of their busy schedules to attend, and for all of their hard work during the week. We hope everyone enjoyed it as much as we did and look forward to our next meeting!

Hollins_Oct18_Award_Labtech India

 

 

 

 

 

 

 

 

Whitley Hypoxystation

The Leukemic Stem Cell Niche: Adaptation to “Hypoxia” Versus Oncogene Addiction

Hematopoietic stem cells (HSC) are responsible for constantly maintaining and replenishing the supply of new blood and immune cells. They give rise to both lymphoid and myeloid progenitor cells, which then proceed to differentiate down their respective paths to form various specialized cells such as erythrocytes, macrophages, B and T cells, to name a few. Within the body, HSCs are found to reside in extremely low oxygen environments called stem cell niches (SCN). Like all other regulated cell cycles, HSCs can lead to cancers such as leukemia and lymphoma if cell division becomes uncontrolled.

MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

Figure 2: Suppression of oncogenic proteins driving non-CML blood neoplasias in the course of cell “adaptation to hypoxia.” MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

In this paper, Cheloni et al. primarily focused on chronic myeloid leukemia (CML).

The authors hypothesized that suppression of the BCR-Abl oncogene is likely a key positive regulator of LSC survival within “hypoxic” SCNs. To analyse the various mechanisms and responses that CML cells demonstrate within SCNs, the authors studied correlations between varied oxygen and glucose concentrations with the amount of BCR-Abl produced. All tests were performed using two human CML cell lines, K562 and KCL22. Testing conditions were precisely controlled to mimic the SCN environment as close as possible. Using a Hypoxystation supplied by Don Whitely Scientific, a water-saturated atmosphere comprising of 0.1% O2, 94.9% N2 and 5% CO2 was generated and maintained.

The research conducted and documented by Cheloni et al. has provided great insight into several key regulatory mechanisms associated with leukemic stem cells as well as an explanation for their notorious reputation for having high relapse rates. Additionally, they established that the triggering of oncogene suppression associated with CML is due to severe energy restriction rather than simply the “adaptation to hypoxia.”

The Don Whitley Scientific Hypoxystation provides the user incredible flexibility as it can control oxygen down to 0.1% while providing a temperature and humidity controlled environment with ample working space. The extreme precision provided by the Hypoxystation was critical to the collection of accurate and reliable data when recreating the demanding environment that comprises SCNs. The atmosphere is constantly monitored and adjusted by the real-time feedback system to ensure accuracy and ISO class 3 clean room HEPA filtration is also available for long term cell culture applications.

Choose your atmosphere with the Hypoxystation hypoxia chamber. Accurately control O2, CO2, Temperature and Humidity.   

Hypoxystation is the only hypoxic chamber purpose built for physiological cell culture research. Specifically designed to create normoxic, hypoxic and anoxic conditions within a controlled and sustained workstation environment, this hypoxic incubator is ideal for research requiring the ability to accurately control O2, CO2, temperature and humidity. The Whitley Internal HEPA Filtration System provides a particle-free internal environment that exceeds ISO 14644 class 3 clean atmosphere.  With such accurate control and the ability to manipulate cells in situ without altering the incubation environment, research into cell biology can be performed over a comprehensive range of oxygen tensions with precision. Don Whitley Scientific offer the following range of Hypoxystations:

                                                                                   

Hypoxystation H35

Hypoxystation H45

Hypoxystation H85

Hypoxystation H135

i2 Instrument Workstation

Application possibilities for Hypoxystation are endless; it is being used for research into tumour microenvironment, hypoxia pathways and HIF signalling, in vitro modelling of in situ environments, cancer cell metastasis, angiogenesis, and many other fields where cells may benefit from a more physiological atmosphere.

 

Hypoxystation range video

New Whitley Hypoxystation Video Launched

Don Whitley Scientific are proud to present a new video that showcases our wide range of Whitley Hypoxic Workstations. The video also features comments from renowned researchers, explaining how a Whitley Hypoxystation benefits their research.

The video is another addition to a growing YouTube channel which features similar videos showcasing our wide product range and company values, including anaerobic and microaerobic workstations, customer testimonials, an introduction to Don Whitley Scientific and the level of service on offer for our customers.

 

 


Researchers featured in this video
Dr Karen Wright – Dr Karen Wright uses a Whitley H35 Hypoxystation in the cell culture laboratory at Lancaster University to expedite a component of her research, namely culturing human gut cells at low physiological levels of oxygen. – Testimonial in full

Dr Michael Cross – Dr Cross and his group use their H35 HEPA Hypoxystation to test cardiovascular drug toxicity in a cardiac spheroid model under physiologically relevant oxygen tensions and also periods of extreme hypoxia/ischemia. – Testimonial in full

 

Click here to explore the full range of Whitley Hypoxystations

 



Contact our sales team for more information on 01274 595728 or email sales@dwscientific.co.uk. For the full range of Whitley products, visit our website at www.dwscientific.co.uk


Whitley Hypoxystation

World Heart Day: Hypoxia in Cardiovascular Disease Research

Cardiovascular disease, including heart disease and stroke, is responsible for approximately 1 in 3 deaths in the US, according to the American Heart Association. World Heart Day on 29 September serves as a platform to educate people on how to take control of their heart health.

Don Whitley Scientific and our US/Canadian distributor HypOxygen would like to take this opportunity to highlight cardiovascular research being carried out around the world – and to say “thank you for being committed to our health.”

Adverse cardiac remodeling after infarction exacerbates myocardial ischemia and increases the likelihood of heart failure. Revuelta-Lopez et al. in Spain present new data showing that in the hypoxic areas of the infarct zone, expression of low-density lipoprotein receptor-related protein 1 (LRP1) is linked to activation of Matrix metalloproteinase (MMP) through Pyk2 phosphorylation, and propose that LRP1 modulation may be a very effective pharmacological target in heart disease. Their H35 Hypoxystation with its controlled low oxygen environment creates physiologically more relevant parameters for cell culture, mimicking ischemia/reperfusion events.

Hypothesizing that Tumor necrosis factor-Related Apoptosis-Inducing Ligand plays a role in ischemic injury during acute myocardial infarction, Jiang et al. have found evidence for a novel immune regulatory mechanism involving TRAIL, ER stress and NF-κB signaling pathways. Culturing their cells in the Hypoxystation H35 at 0.3% oxygen allowed the lab to simulate the ischemia/reperfusion processes that cause cardiomyocyte loss and increase mortality in Coronary Heart Disease.

Hypoxia in the embryonic environment supports maintenance of a primitive glycosaminoglycan-rich heart valve matrix, the specific composition of which determines proper function, and as hypoxia decreases after birth, the extracellular matrix matures. Amofa et al. at Cincinnati’s Children’s Medical Center, using the H35 Hypoxystation, provide new data that exposure of adult heart tissue to hypoxia induces hyaluronan remodeling, GAG accumulation, and degeneration of the extracellular matrix in the heart valve, effects that are implicated in Myxomatous mitral valve disease.

Dr. Michael Cross, Molecular and Clinical Pharmacology Department, University of Liverpool, says of his work with cardiac spheroids : “The H35 allows us to generate oxygen levels that reflect the in vivo physiology these cells would be exposed to. We chose the Hypoxystation with its oxygen profiling feature, which allows us to recreate cycles of ischemia, where oxygen levels typically sink to 1-3%”.

Revuelta-Lopez et al 2017

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Image from: Revuelta-Lopez et al. “Relationship among LRP1 expression, Pyk2 phosphorylation and MMP-9 activation in left ventricular remodelling after myocardial infarction” J Cell Mol Med. 2017 Sep;21(9):1915-1928

 

Amofa et al 2017

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Hypoxia increases GAGs, Sox9 nuclear localization and Hyal2 expression in cAVOCs.

Image from: Amofa et al. (2017) “Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves” Am J Physiol Heart Circ Physiol. 2017 Aug 25:ajpheart.00209.2017

 

 

 

A35_Front

Busy June for Don Whitley Scientific

Don Whitley Scientific have a busy month in June with several exhibitions and events around the UK. Here’s a rundown on where you might see us and the equipment that we’ll be showing at these events.

Rapid Micro Methods – Campden BRI – 8th June – More info
The first meeting in June that Don Whitley Scientific will attend is the Rapid Micro Methods meeting at Campden BRI. This meeting features talks that cover rapid methods, automation and miniaturisation in microbiology. Don Whitley Scientific will be exhibiting a WASP Touch, ProtoCOL3 Plus and Masterclave 20.

 

Welsh Microbiology Association Summer Meeting – Village Hotel, Swansea – 9th June – More info
This one day event gathers some of the most important figures working in Welsh microbiology. Don Whitley Scientific will be exhibiting a WASP Touch and ProtoCOL3 at the event.

BACR Tumour Microenvironment – Nottingham Conference Centre – 14th-16th June – More info
This meeting aims to highlight the latest advances in this field from basic science to clinical development and to provide a workshop forum for researchers to discuss recent developments in 3D cancer modelling. Don Whitley Scientific will be exhibiting a Whitley H35 Hypoxystation at this meeting, a market leading workstation for working with cancer cells in a physiologically relevant atmosphere.

Clinisys Summer Conference – The Belfry, Birmingham – 14th-16th June –  More info
The CliniSys Summer  Conference and user group meeting will once again be hosted at The Belfry on the 15th & 16th June. More than 250 delegates are expected to attend to hear thought-provoking presentations and discussions with some of the key innovators and thought leaders in healthcare and laboratory medicine. Don Whitley Scientific will be exhibiting a Chromazona on their stand, as well as an interactive display featuring all Don Whitley Scientific Workstations

Association for Radiation Research 2017 – St Hilda’s College, Oxford – 27th-28th June – More info
This year’s ARR meeting will be focusing on “Improving radiotherapy response through radiation research”.  The meeting programme features talks from academics from around the world, speaking on subjects relevant to radiotherapy and radiation. Don Whitley Scientific will have a Whitley H35 Hypoxystation on show at this event.

CHROMAZONA Automatic Colony Counter

BSMT Annual Scientific Conference

Don Whitley Scientific will be exhibiting at the British Society for Microbial Testing annual conference, held on 12th May at Public Health England in Colindale. 

The BSMT conference is aimed at senior biomedical and clinical scientists, other scientists and medical microbiologists. The meeting will feature talks on a wide range of relevant microbiology topics. The programme features UK and international speakers, who will deliver a range of talks on the day. Don Whitley Scientific will be there to exhibit at the event. Sales representatives will be on hand to discuss how our product range can benefit a range of microbiology applications.

The featured product on the Don Whitley Scientific exhibition stand will be the ChromaZona, an automated microbial identification and antibiotic susceptibility testing (AST) system, which provides faster results for busy laboratories. ChromaZona features automatic comparison with EUCAST MIC breakpoint values and provides fully traceable information for UKAS accreditation.

Also on the Don Whitley Scientific stand is the Whitley Interactive Product Presentation. This interactive display will give delegates the option to explore the full range of Whitley Workstations and access in-depth information on each product. Make sure to visit the Don Whitley Scientific exhibition stand if you are attending this event.

jane-freeman-thumbnail

Why Choose a Whitley Workstation?

There are many reasons to choose a Whitley Workstation when it comes to Anaerobic, Hypoxic or Microaerophilic work. We can discuss these with you anytime, but we also have plenty of satisfied customers who have expressed why using a Whitley Workstation improves their working methods and results.

Over the years, customers have supplied us with many testimonials about their Don Whitley Scientific products. From these we can see that not only have Whitley Workstations become approved by fantastic researchers worldwide, but we can also help promote the amazing work that is done by our customers.

Dr Vaibhao Janbandhu at the Victor Chang Cardiac Research Institute (VCCR) in Sydney, Australia uses a Whitley H35 Hypoxystation in his work on finding new ways to stimulate heart regeneration during ageing and after heart attack. He uses his H35 Hypoxystation to isolate, culture and characterise adult cardiac stem cells. In Dr Janbandhu’s words the H35 is “an integral part of the project to advance the project aims”.

In this video testimonial, Jane Freeman at Leeds General Infirmary explains how her Whitley A95 Workstation improves the working methods in her Clostridium difficile research. Jane reports that she and her team are able to use the workstation for “several hours at a time in relative comfort” and that the workstation is able to house all the technical equipment her team requires. This allows “the whole experiment to be performed in optimum conditions without introducing air at all”. Jane explains that “reliability, versatility and space are the significant benefits of the workstations in our work on Clostridium difficile“.

The Institute of Cancer Research in London is one of the world’s most influential research institutes, with an outstanding record of achievement dating back more than 100 years. At the Institute, George Poulogiannis uses a combination of Whitley i2 Instrument Workstation (with Seahorse XF Analyzer) and Whitley H35 Hypoxystation in his research into breast cancer. Hypoxia is a key factor in the “Hallmarks of Cancer” and this team are studying the role of hypoxia in cell invasion and metastasis, oncogene-induced senescence and resistance to current treatment options. The i2 and H35 replicate a physiologically relevant atmosphere for these studies, enabling consistent and reliable results. This combination of Workstations is also used by Dr Ayse Latif, who is researching gynaecological cancers at The University of Manchester.

Don Whitley Scientific would like to take this opportunity to thank all customers who have provided testimonials. If you would be interested in supplying a testimonial, please contact Alex_Rhodes@dwscientific.co.uk.

Take a look at our other testimonials

IMG_2384

Workstation Installation at Trinity College Dublin

Don Whitley Scientific and our distributor in Ireland, Davidson and Hardy, recently installed equipment at Trinity College Dublin to help with research into cell metabolism.

Stephen Maher, Assistant Professor at Trinity College Dublin first experienced using a Whitley Workstation when working with a group at The University of Hull.  In his Trinity College Dublin lab, Don Whitley Scientific recently installed a Whitley H35 Hypoxystation, to be used connected to a Whitley i2 Instrument Workstation. The combination of these two units should help Stephen Maher greatly in his research.

The Whitley H35 Hypoxystation is ideal for cell and tissue culture researchers who want to accurately control oxygen, carbon dioxide, temperature and humidity. A removable front allows large quantities of samples or pieces of equipment to be placed inside the unit for use within these specific, physiologically relevant conditions. The i2 Workstation was developed in response to a rising number of enquiries from scientists wanting to use Seahorse Extracellular Flux (XF) Analyzers in hypoxic conditions and were dissatisfied with the solutions available. This workstation can be used as a stand-alone unit or connected to a Whitley Hypoxystation via the new Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

 

IMG_2379

 

Explore the Whitley Hypoxystation Range

20170124_150718 cropped

Don Whitley Visits The Francis Crick Institute

Dr Don Whitley, chairman and founder of DWS, recently visited The Francis Crick Institute in London to see the recently installed Whitley H45 Hypoxystation at the site. 

The Crick has moved into a brand new state-of-the-art building in the centre of London. Situated next to Kings Cross and St Pancras stations, The Crick brings together 1500 scientists and staff working collaboratively in the biggest biomedical research facility under one roof in Europe. The work at The Crick covers many disciplines and applications in biomedical research, all with the aim of improving understanding of human health and disease.

 

Don Whitley established Don Whitley Scientific in 1976 and today Don Whitley Scientific Limited is a leading international supplier of innovative equipment and services to the microbiology and tissue culture industries. Recently DWS installed a H45 Hypoxystation into the institute, and Don Whitley went to visit the new customer with a member of the sales team.

20170124_145656 cropped

Don Whitley (far right) with one of the groups that will be using the Whitley H45 Hypoxystation

The Whitley H45 Hypoxystation has sufficient space to accommodate a variety of equipment whilst still providing generous working and incubation areas. Whitley Hypoxystations can be equipped with a range of unique options and features, including CO2 monitoring and automatic dehumidification fitted as standard, features that will make working with the H45 easy and efficient for the team at The Crick.

With his name featured on products in hundreds of clinical and research laboratories worldwide, it can be said that the staff at The Crick were excited to meet Don Whitley himself. The excitement was shared by Don, who enjoyed looking around one of the most exciting centres for biomedical research in the UK and taking a few pictures along the way.

 

20170124_152554

Don takes a photo looking out over the atrium of The Francis Crick Institute

 

2016-05-18 15.10.56

Whitley H35 Hypoxystation used in heart regeneration project

Dr Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCRI) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).

Dr Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.

Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr Janbandhu’s work as they accurately replicate the in vivo environment.

His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.

As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.

Dr Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.

Dr Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.

2016-05-18 15.13.27