DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Posts Tagged ‘HEPA Workstation’

H35 HEPA Testimonial Video

In this video, Dr Michael Cross from the MRC Centre for Drug Safety Science, University of Liverpool, discusses his current research involving the incubation of cardiac spheroids.

Dr Cross uses a Whitley H35 HEPA Hypoxystation, and explains the benefit of the HEPA filtration system in providing a sterile environment for the prolonged culture of cells in physiologically relevant conditions.

For more information about Don Whitley Scientific’s unique HEPA filter, click here.

 

 

Data logging

Did You Know: about Data Logging?

Do you need to record the environmental parameters inside your workstation? All of our touch-screen models display this information in graphical format for the user to see, and the data logging option enables this data to be downloaded via a USB interface to a memory stick and transferred to a PC for further analysis.

It is also possible to download an events log if required.

Which Whitley Workstations have this option?
Data logging is compatible with all of our touch-screen workstations.

Which parameters can I record?
 

 HypoxystationAnaerobic
Workstation
Oxygen✔*
Carbon Dioxide
Temperature
Humidity
Cabinet Internal Pressure

*If anaerobic conditions monitor is fitted

 

How long does it take to download the data?
The information download takes approximately 20 seconds.

Where is the USB port located?
The port is located on a panel above the airlock, and is only accessible when the airlock door is open. This ensures that the memory stick can never be forgotten – as the door cannot close unless the memory stick has been removed.

What does the events log comprise?
The events log monitors the different processes and actions that run in the machine and flags up any errors. It also adds traceability to the system by recording how it is used.

These are some of the processes monitored:

 HypoxystationAnaerobic
Workstation
Low Gas Pressure
Inner & Outer Airlock Door Open/Closed
Elevated Oxygen Level
Airlock Cycle
Oxygen Calibration
Oxygen Feedback
Sleeve Vacuum/ Gassing

 Please contact us for more information.

 

 

 

Whitley H35 Hypoxystation

Biological Containment

Looking for extra biological containment from your workstation?

If you need additional biological containment within your HEPA filtered Workstation, there is now an option to reduce biological discharge from the chamber via the gas outlets. The Whitley Enhanced Biological Containment System involves fitting HEPA filters to the two gas outlets on a Whitley HEPA Workstation.

Laboratory tests were conducted on an H35 Hypoxystation to deliberately challenge the exhaust filters with high concentrations of aerosolized bacterial cells and quantify their discharge through the exhaust valve filters.

The results of this experiment demonstrated that the system achieved a substantial reduction in bacterial loading (better than 99.999%) within the discharged gas. A paper detailing the full methodology and results of this study can be found here.

Storage Rack with Flasks

New Storage Rack Available

 

Here’s a new storage rack that we have designed for use with our Hypoxystation range. Manufactured from aluminium and finished in a durable white powder coating, this storage facility is comprised of three shelves and can accommodate up to 6 multi-well plates, twelve T25 tissue culture flasks or a combination of these and other labware.

Storing your samples in this manner allows the atmosphere to circulate evenly and thereby optimises the conditions for your cells. It is also freezer and incubator-safe.

Contact us for more information and quote product code A03780

Whitley H35 Hypoxystation

Did You Know: about HEPA Filtration?

HEPA, or High Efficiency Particulate Air, is a filtration system which ensures the utmost product protection for your samples.

Unlike competitor systems that draw atmosphere out of the workstation before filtering and reintroducing it, Don Whitley Scientific has created a unique design, which is housed completely inside the workstation. Housing the HEPA system inside the chamber prevents the build-up of moisture which renders HEPA filters ineffective, and cleans the internal atmosphere quickly.

Using innovative circulation technology, all the atmosphere in the chamber passes through the filter 900 times every hour. This quickly creates and maintains an environment exceeding the level of atmospheric cleanliness stipulated by ISO 14644, referenced in the Cell Tissue Culture Directive as the recommended standard for the handling of mammalian cells. We achieve levels of atmospheric cleanliness exceeding the requirements of Class 3 of this standard, which to the best of our knowledge is better than any other manufacturer.

There are currently three Whitley Workstations available with HEPA filtration:

A35 HEPA Anaerobic Workstation
H35 HEPA Hypoxystation
H45 HEPA Hypoxystation.

Proof of the atmospheric cleanliness achieved during the rigorous tests conducted on every HEPA workstation before dispatch is available on request. Please contact us for more information.

This video demonstrates the effectiveness of our system.

Laboratory Standard Shot DYK Blog

Did you know: How best to clean your workstation?

 

 

This is the 10th in our ‘Did you know’ series of articles introducing things you may not know about the Whitley range of products and services – read on and you may find out something about your workstation that could make your working life easier.

We regularly receive enquiries from customers with regard to which disinfectants can be safely used inside Whitley Workstations. Although it is not practicable to evaluate every available product, we have tested the materials compatibility of several sporicidal disinfectants and can now provide comprehensive advice on this topic.

 

 

How often should I clean my workstation?

DWS recommend that as part of the daily checks, users ensure that the workstation is free from spillage and cleaning carried out as necessary. Prior to having your workstation serviced, however, it may be necessary for it to be decontaminated.

Which disinfectants have DWS tested?

  • Spor-Klenz – peracetic acid (approx 0.1%) + hydrogen peroxide (approx 1.0%) – “ready to use” product, tested without further dilution.
  • Chemgene HLD4H – quaternary ammonium compounds (approx 1%) + chlorhexidine digluconate (approx 1%) – “ready to use” product, tested without further dilution.
  • Safe Hospital – hypochlorous acid – “ready to use” product, tested without further dilution.
  • Tristel Fuse – chlorine dioxide (approx 1%) – prepared from concentrated components according to manufacturer’s instructions.

For more information on these tests, please see the appendices of your Whitley Workstation user manual.

 

Which of the above products are OK for use in a Whitley Workstation?

Our tests demonstrated that each of these formulations is satisfactory for use in Whitley Workstations and will not cause any damage when used in accordance with the manufacturer’s recommendations.

 

What if I want to use a cleaning agent not on the above list?

Other disinfectants containing the active ingredients listed above, at similar concentrations to those shown, can also be safely used inside Whitley Workstations. If you wish to use a disinfectant containing active ingredients not listed here, please contact us for advice before proceeding.

 

Why should I contact DWS for advice – will any damage not be covered under my warranty?

Unfortunately, we cannot cover under the equipment’s warranty any damage caused to a Whitley Workstation as a result of exposure to products that have not been approved by us.

 

Can I use Virkon as my preferred sanitising solution?

After a thorough study lasting 20 weeks, we concluded that the presence of any uncovered containers of Virkon in anaerobic and variable atmosphere workstations leads to the degradation of stainless steel, brass and some other metal components. The effect is more considerable when oxygen is present in the selected gas mixture. These conclusions are supported by technical information available on the Virkon manufacturer’s website and independent observations by an Institute of Materials metallurgist. If Virkon is the preferred sanitising solution these adverse effects can be minimised by ensuring that Virkon within any workstation is always kept in a covered vessel when not in use.

 

OK, so what’s the best way to clean the acrylic parts of a Whitley Workstation?Labdet100 Blog

The transparent and/or white acrylic on the inside and outside of the Whitley Workstation System may be swabbed with a 2% solution of Labdet 100 (DWS – stock code D00003) in warm water and dried afterwards with a soft clean cloth. In the case of culture spillages then any of the disinfectants listed above solution should be applied to the spillage and left for 30 minutes. It should then be mopped.

 

Never use any solvent on the acrylic surfaces of the workstation. Use only water and a mild detergent solution (i.e. Labdet 100 2% solution) as a cleaning agent.

 

What if the spillage leaks underneath the floor of the workstation?

If spillage is not contained on the working surface then access underneath this area will be required in all Whitley Workstations except the DG250. Some workstations require the removal of retaining bolts before the floor can be lifted up or pivoted for cleaning underneath.

If liquid has been spilled upon the Anotox and catalyst sachets they should be removed, dried and/or replaced.

 

How can I deal with scratches on the acrylic plastic surface of my workstation?

Scratches may be removed by gently polishing the surface with ‘DURAGLIT WADDING’ followed by wiping with a soft clean cloth. Deep scratches may require the use of Wet and Dry abrasive paper used wet, followed by polishing with ‘DURAGLIT’ – seek advice from Don Whitley Scientific Limited or our authorised agents overseas.

 

How can I clean the sleeves on my workstation?Sleeve Kit-Components DYK Blog

The white sleeves used on all Whitley Workstations may require cleaning after periods of extended use. There are a number of options for cleaning and some recommendations listed below:

  • Wipe with a mild detergent
  • Wash or dry-clean with a detergent up to 75ºC
  • For disinfection use Sodium Hypochlorite (at no more than 1000ppm active Chlorine) or any of the disinfectants listed above
  • Do not use Biological or Phenolic based cleaners
  • Wash and dry completely before storage
  • Some surface wrinkling may occur.

 

If you have any remaining concerns about disinfectants for use in Whitley Workstations, please get in touch with our sales team:

Tel: 01274 595728

Or

Email: sales@dwscientific.co.uk

 

Burga Kalz Fuller

Fast Growing Hypoxia Technology Company Strengthens Resources

Don Whitley Scientific announce a key new appointment at their US distributor, HypOxygen (Frederick, MD, USA). Burga Kalz Fuller, PhD has joined the firm to further enhance their penetration of the Hypoxystation hypoxic workstation and related products into the growing field of mammalian cell based hypoxia research.

Burga comes with a wealth of experience within the life sciences industry, having spent the last ten years working with technology suppliers both in Europe and the United States where she was instrumental in the technical supervision of product and equipment development and marketing strategies.

Burga joins HypOxygen as Product Manager to continue HypOxygen’s recent success in providing state of the art hypoxic workstations that lead the way in advancing scientific research and innovation.

Burga comments, “I am excited to start a new challenge with HypOxygen and look forward to working with the team.  The range of Whitley HEPA Hypoxystations will play a critical role in furthering advances in the fields of cancer and regenerative medicine research due to the wealth of special features for ultra-clean, precisely controlled physiological atmosphere that make it truly special.  I am fortunate to be joining such a respected company that prides itself on providing industry leading technologies to the growing fields of tissue culture research and hypoxia.”

Jason Cadle, National Sales Manager at HypOxygen, said “Burga’s wealth of molecular cell biology experience and industry knowledge has already made her a key addition to the HypOxygen family. We view her appointment as a sign of our commitment to being the leading company in our industry. Our new innovations and the increasing demand from researchers led us to look for an addition to our team who will fit in with our ethos of innovation and exceptional service. It is very fortunate that we were able to find someone of Burga’s caliber to act in this capacity. I’m confident that Burga will play a key role in providing and implementing high quality technical solutions for our clients.”

Paul Walton, Managing Director of Don Whitley Scientific, added “Burga’s appointment is great news for the Whitley Hypoxystation range of products as we improve and expand the range. Having Burga on the team at HypOxygen will improve our international presence in terms of the service we can provide to all our distributors worldwide.”

Whitley H35 HEPA Hypoxystation

HEPA Filtered Hypoxystation for New Year Launch

If you need to work in a HEPA filtered workstation environment – with precise control of oxygen, carbon dioxide, temperature and humidity, for example in stem cell research – this Hypoxystation is for you.

A new Hypoxystation complete with a HEPA filtration system is due to launch in the New Year. Early test results have indicated that the Whitley H35 HEPA Hypoxystation will achieve Class 2 conditions as per the redefined ISO standards for particulate count and classification for air cleanliness – this will be independently verified in due course.

The H35 HEPA Hypoxystation will look exactly like the current H35 but will be deeper by 90mm. The dimensions will therefore be 1255 x 810 x 710mm (w x d x h). This product will have a removable front as standard, fitted with two oval, sleeved ports. CO2 monitoring will be included as standard on this product, a saving of over £2,000.

Options/accessories, literature and specification details will be published on www.dwscientific.co.uk in January 2014.

For further information please contact your sales representative or email us on sales@dwscientific.co.uk