DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Posts Tagged ‘H45 Hypoxystation’

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Don Whitley Scientific Exhibit at Key Cardiovascular Meeting

Don Whitley Scientific recently attended the BSCR (British Society of Cardiovascular Research) Annual Meeting, held this year at The University Of Leeds. The focus of the meeting was on “Cardiovascular Disease and Diabetes” and the event featured fantastic talks and poster presentations from renowned figures in Cardiovascular research.

The Don Whitley Scientific stand featured a H45 Hypoxystation. The H45 is a large, three port cell culture workstation, ideal for replicating physiologically relevant atmospheres through the control of oxygen, temperature and humidity. Working in a Hypoxystation means cell cultures and other samples are within a stable atmosphere that matches the specific microenvironments that exist inside the human body, meaning research results are dependable and accurate.

 

At The University of Liverpool, Dr Michael Cross is conducting cardiovascular research using a Whitley H35 HEPA Hypoxystation as he looks at Low Oxygen Drug Toxicity Testing in Cardiac Spheroids

The Whitley Hypoxystation range can be viewed here

Whitley H35 Hypoxystation

TRACER mimics oxygen and nutrient gradients in tumours

Our US/Canada distributor, HypOxygen, wanted to find out more about Whitley H35 and H45 Hypoxystation customer Brad Wouters’ recent research, especially the newly published paper describing his three-dimensional tumour TRACER project, published as “A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients“ (Rodenhizer et al., Nature Materials 15, 227–234, 2016).

Interview with Brad Wouters, Princess Margaret Cancer Center, Toronto.  March 4, 2016

“The paper describes a new device that enables us to create naturally occurring oxygen gradients, such as the ones found in tumours. The TRACER (engineered tumour roll for analysis of cellular environment and response) developed by Alison McGuigan of the University of Toronto is basically an engineered tumour that is assembled by rolling a single-component biocomposite sheet. Respiration-induced oxygen and metabolite gradients are established inside the device, so it’s an alternative to other 3D models such as spheroids. The benefit is that the TRACER rolls can be rapidly disassembled, enabling you to interrogate the biological phenotypes or properties, characteristics, and metabolites of the cells in those defined locations within the gradient, in the six layers of the tracer. The cells can be removed in a variety of ways; you can remove the cells in a viable way and process them as you would any cells, to do flow cytometry, metabolomics, cell survival, proliferation.

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AACR logo

AACR Poster Session

A team of scientists from the Brain Sciences Division, Hammersmith Campus, Imperial College London, have successfully submitted an abstract to the AACR, and their poster will be presented at the upcoming meeting in Philadelphia, April 18-22.

Some of the research for this poster, entitled “Arginine deprivation using ADI-PEG20 leads to regression of an ASS1-ve intracranial GBM tumor in mice and potentiates gamma irradiation of ASS1+ve GBM in vitro”, was conducted in a Whitley H45 HEPA Hypoxystation.

The poster will be on display at the Late-Breaking Research: Experimental and Molecular Therapeutics session, Sunday April 19, 2015 between 1 PM and 5 PM.

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TShirt for Survey

Free T-Shirt: Hypoxia Research

 

Do you culture cells in hypoxic conditions? For a limited time only, you can earn a free t-shirt by taking a few minutes to answer our questions and help us to better fulfil your cell culture needs.

We want to know what your research focus is, how you conduct your cell culture and how you think oxygen content might influence aspects of your cell culture.

Please click here to complete a very short online form.

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One City, Two Cultures

HypOxygen and Don Whitley Scientific are sponsoring a series of Keystone Symposia dealing with tumour micro environment (Vancouver), mitochondria and metabolism (Santa Fe), and immunity and inflammation (Olympic Valley). At the joint Keystone symposium on “Mitochondria, Metabolism and Heart Failure” and “Diabetes and Metabolic Dysfunction” in Santa Fe, research groups have come together to present their latest findings on metabolism.

Mitochondrial function is central to metabolism and energy sensing of the cells in all organs, and this joint meeting is bringing together researchers who gave talks on the regulation of metabolism under stress, mitochondrial quality control and dysregulated gluconeogenesis. Insight into the genes and the pathways controlling these processes at the cellular level is driving the search for more effective drugs.

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Did You Know: Whitley Workstations are Ethernet-enabled for Remote Access?

Have you ever wanted to check the status of your Whitley Workstation when you were away from the lab? Well now you can. Most of our new workstations are Ethernet-enabled so you can dial in and access the touch-screen control panel from the other side of the building, or even from the other side of the world. This allows you to check the current operating conditions of the workstation and, if activated, view any of the graphical screens. If necessary, you can then make changes to the parameters, i.e. temperature, humidity, O2 and CO2 levels from wherever, whenever!

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Whitley H35 Hypoxystation

Did You Know: about HEPA Filtration?

HEPA, or High Efficiency Particulate Air, is a filtration system which ensures the utmost product protection for your samples.

Unlike competitor systems that draw atmosphere out of the workstation before filtering and reintroducing it, Don Whitley Scientific has created a unique design, which is housed completely inside the workstation. Housing the HEPA system inside the chamber prevents the build-up of moisture which renders HEPA filters ineffective, and cleans the internal atmosphere quickly.

Using innovative circulation technology, all the atmosphere in the chamber passes through the filter 900 times every hour. This quickly creates and maintains an environment exceeding the level of atmospheric cleanliness stipulated by ISO 14644, referenced in the Cell Tissue Culture Directive as the recommended standard for the handling of mammalian cells. We achieve levels of atmospheric cleanliness exceeding the requirements of Class 3 of this standard, which to the best of our knowledge is better than any other manufacturer.

There are currently three Whitley Workstations available with HEPA filtration:

A35 HEPA Anaerobic Workstation
H35 HEPA Hypoxystation
H45 HEPA Hypoxystation.

Proof of the atmospheric cleanliness achieved during the rigorous tests conducted on every HEPA workstation before dispatch is available on request. Please contact us for more information.

This video demonstrates the effectiveness of our system.

Laboratory Standard Shot DYK Blog

Did you know: How best to clean your workstation?

 

 

This is the 10th in our ‘Did you know’ series of articles introducing things you may not know about the Whitley range of products and services – read on and you may find out something about your workstation that could make your working life easier.

We regularly receive enquiries from customers with regard to which disinfectants can be safely used inside Whitley Workstations. Although it is not practicable to evaluate every available product, we have tested the materials compatibility of several sporicidal disinfectants and can now provide comprehensive advice on this topic.

 

 

How often should I clean my workstation?

DWS recommend that as part of the daily checks, users ensure that the workstation is free from spillage and cleaning carried out as necessary. Prior to having your workstation serviced, however, it may be necessary for it to be decontaminated.

Which disinfectants have DWS tested?

  • Spor-Klenz – peracetic acid (approx 0.1%) + hydrogen peroxide (approx 1.0%) – “ready to use” product, tested without further dilution.
  • Chemgene HLD4H – quaternary ammonium compounds (approx 1%) + chlorhexidine digluconate (approx 1%) – “ready to use” product, tested without further dilution.
  • Safe Hospital – hypochlorous acid – “ready to use” product, tested without further dilution.
  • Tristel Fuse – chlorine dioxide (approx 1%) – prepared from concentrated components according to manufacturer’s instructions.

For more information on these tests, please see the appendices of your Whitley Workstation user manual.

 

Which of the above products are OK for use in a Whitley Workstation?

Our tests demonstrated that each of these formulations is satisfactory for use in Whitley Workstations and will not cause any damage when used in accordance with the manufacturer’s recommendations.

 

What if I want to use a cleaning agent not on the above list?

Other disinfectants containing the active ingredients listed above, at similar concentrations to those shown, can also be safely used inside Whitley Workstations. If you wish to use a disinfectant containing active ingredients not listed here, please contact us for advice before proceeding.

 

Why should I contact DWS for advice – will any damage not be covered under my warranty?

Unfortunately, we cannot cover under the equipment’s warranty any damage caused to a Whitley Workstation as a result of exposure to products that have not been approved by us.

 

Can I use Virkon as my preferred sanitising solution?

After a thorough study lasting 20 weeks, we concluded that the presence of any uncovered containers of Virkon in anaerobic and variable atmosphere workstations leads to the degradation of stainless steel, brass and some other metal components. The effect is more considerable when oxygen is present in the selected gas mixture. These conclusions are supported by technical information available on the Virkon manufacturer’s website and independent observations by an Institute of Materials metallurgist. If Virkon is the preferred sanitising solution these adverse effects can be minimised by ensuring that Virkon within any workstation is always kept in a covered vessel when not in use.

 

OK, so what’s the best way to clean the acrylic parts of a Whitley Workstation?Labdet100 Blog

The transparent and/or white acrylic on the inside and outside of the Whitley Workstation System may be swabbed with a 2% solution of Labdet 100 (DWS – stock code D00003) in warm water and dried afterwards with a soft clean cloth. In the case of culture spillages then any of the disinfectants listed above solution should be applied to the spillage and left for 30 minutes. It should then be mopped.

 

Never use any solvent on the acrylic surfaces of the workstation. Use only water and a mild detergent solution (i.e. Labdet 100 2% solution) as a cleaning agent.

 

What if the spillage leaks underneath the floor of the workstation?

If spillage is not contained on the working surface then access underneath this area will be required in all Whitley Workstations except the DG250. Some workstations require the removal of retaining bolts before the floor can be lifted up or pivoted for cleaning underneath.

If liquid has been spilled upon the Anotox and catalyst sachets they should be removed, dried and/or replaced.

 

How can I deal with scratches on the acrylic plastic surface of my workstation?

Scratches may be removed by gently polishing the surface with ‘DURAGLIT WADDING’ followed by wiping with a soft clean cloth. Deep scratches may require the use of Wet and Dry abrasive paper used wet, followed by polishing with ‘DURAGLIT’ – seek advice from Don Whitley Scientific Limited or our authorised agents overseas.

 

How can I clean the sleeves on my workstation?Sleeve Kit-Components DYK Blog

The white sleeves used on all Whitley Workstations may require cleaning after periods of extended use. There are a number of options for cleaning and some recommendations listed below:

  • Wipe with a mild detergent
  • Wash or dry-clean with a detergent up to 75ºC
  • For disinfection use Sodium Hypochlorite (at no more than 1000ppm active Chlorine) or any of the disinfectants listed above
  • Do not use Biological or Phenolic based cleaners
  • Wash and dry completely before storage
  • Some surface wrinkling may occur.

 

If you have any remaining concerns about disinfectants for use in Whitley Workstations, please get in touch with our sales team:

Tel: 01274 595728

Or

Email: sales@dwscientific.co.uk

 

Pipette and pipette filler in a Whitley Airlock

Did you know: Pipettes fit into a 12 litre airlock

This is the ninth in our ‘Did you know’ series of articles introducing things you may not know about the Whitley range of products and services – read on and you may find out something about your workstation that could make your working life easier.

Did you know that 10ml pipettes and pipette fillers fit comfortably into a Hypoxystation 12 litre airlock? It might be small enough that it takes only 60 seconds to perform a full cycle but the Hypoxystation airlock has been designed with your working accessories in mind.

The 12 litre airlock will also accommodate:

  • Up to 44 x 96 well plates
  • Up to 84 x T25 culture flasks
  • Up to 8 x T175 culture flasks

 

LumaScope in Whitley Airlock

LumaScope in Whitley Airlock

What other items can a Whitley Airlock accommodate?

The Hypoxystation airlock accommodates a range of other laboratory equipment, for example, the  LumaScope™ Live Cell Imaging Platform. It is easy to transfer into and out of the workstation using the airlock (as shown in the picture) and can sit underneath the shelf, to be pulled to the front of the chamber for use. If your Hypoxystation has a half shelf, the LumaScope™ can sit to one side and be ready for regular use.

What if I have a particular item in mind for use in a Hypoxystation?

If you have any concerns about equipment and accessories fitting into a Whitley Airlock, or would like to discuss a particular item that you would like to use inside a Hypoxystation chamber, please get in touch with our sales team:

Whitley Hypoxystation 12 Litre Airlock

Whitley Hypoxystation 12 Litre Airlock

Tel: 01274 595728

Or

Email: sales@dwscientific.co.uk

 

 

 

 

 

Whitley H35 Hypoxystation

How to Calibrate the Oxygen Cell on a Hypoxystation

Here’s a link to a video on YouTube explaining how to calibrate the oxygen cell on a Whitley Hypoxystation: https://www.youtube.com/watch?v=WJ_wwVnGgfc&feature=youtu.be