DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


Contact Us +44 (0) 1274 595728 sales@dwscientific.co.uk

Follow Don Whitley Scientific

Posts Tagged ‘H35 Hypoxystation’

Whitley Hypoxystation

World Heart Day: Hypoxia in Cardiovascular Disease Research

Cardiovascular disease, including heart disease and stroke, is responsible for approximately 1 in 3 deaths in the US, according to the American Heart Association. World Heart Day on 29 September serves as a platform to educate people on how to take control of their heart health.

Don Whitley Scientific and our US/Canadian distributor HypOxygen would like to take this opportunity to highlight cardiovascular research being carried out around the world – and to say “thank you for being committed to our health.”

Adverse cardiac remodeling after infarction exacerbates myocardial ischemia and increases the likelihood of heart failure. Revuelta-Lopez et al. in Spain present new data showing that in the hypoxic areas of the infarct zone, expression of low-density lipoprotein receptor-related protein 1 (LRP1) is linked to activation of Matrix metalloproteinase (MMP) through Pyk2 phosphorylation, and propose that LRP1 modulation may be a very effective pharmacological target in heart disease. Their H35 Hypoxystation with its controlled low oxygen environment creates physiologically more relevant parameters for cell culture, mimicking ischemia/reperfusion events.

Hypothesizing that Tumor necrosis factor-Related Apoptosis-Inducing Ligand plays a role in ischemic injury during acute myocardial infarction, Jiang et al. have found evidence for a novel immune regulatory mechanism involving TRAIL, ER stress and NF-κB signaling pathways. Culturing their cells in the Hypoxystation H35 at 0.3% oxygen allowed the lab to simulate the ischemia/reperfusion processes that cause cardiomyocyte loss and increase mortality in Coronary Heart Disease.

Hypoxia in the embryonic environment supports maintenance of a primitive glycosaminoglycan-rich heart valve matrix, the specific composition of which determines proper function, and as hypoxia decreases after birth, the extracellular matrix matures. Amofa et al. at Cincinnati’s Children’s Medical Center, using the H35 Hypoxystation, provide new data that exposure of adult heart tissue to hypoxia induces hyaluronan remodeling, GAG accumulation, and degeneration of the extracellular matrix in the heart valve, effects that are implicated in Myxomatous mitral valve disease.

Dr. Michael Cross, Molecular and Clinical Pharmacology Department, University of Liverpool, says of his work with cardiac spheroids : “The H35 allows us to generate oxygen levels that reflect the in vivo physiology these cells would be exposed to. We chose the Hypoxystation with its oxygen profiling feature, which allows us to recreate cycles of ischemia, where oxygen levels typically sink to 1-3%”.

Revuelta-Lopez et al 2017

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Image from: Revuelta-Lopez et al. “Relationship among LRP1 expression, Pyk2 phosphorylation and MMP-9 activation in left ventricular remodelling after myocardial infarction” J Cell Mol Med. 2017 Sep;21(9):1915-1928

 

Amofa et al 2017

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Hypoxia increases GAGs, Sox9 nuclear localization and Hyal2 expression in cAVOCs.

Image from: Amofa et al. (2017) “Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves” Am J Physiol Heart Circ Physiol. 2017 Aug 25:ajpheart.00209.2017

 

 

 

jane-freeman-thumbnail

Why Choose a Whitley Workstation?

There are many reasons to choose a Whitley Workstation when it comes to Anaerobic, Hypoxic or Microaerophilic work. We can discuss these with you anytime, but we also have plenty of satisfied customers who have expressed why using a Whitley Workstation improves their working methods and results.

Over the years, customers have supplied us with many testimonials about their Don Whitley Scientific products. From these we can see that not only have Whitley Workstations become approved by fantastic researchers worldwide, but we can also help promote the amazing work that is done by our customers.

Dr Vaibhao Janbandhu at the Victor Chang Cardiac Research Institute (VCCR) in Sydney, Australia uses a Whitley H35 Hypoxystation in his work on finding new ways to stimulate heart regeneration during ageing and after heart attack. He uses his H35 Hypoxystation to isolate, culture and characterise adult cardiac stem cells. In Dr Janbandhu’s words the H35 is “an integral part of the project to advance the project aims”.

In this video testimonial, Jane Freeman at Leeds General Infirmary explains how her Whitley A95 Workstation improves the working methods in her Clostridium difficile research. Jane reports that she and her team are able to use the workstation for “several hours at a time in relative comfort” and that the workstation is able to house all the technical equipment her team requires. This allows “the whole experiment to be performed in optimum conditions without introducing air at all”. Jane explains that “reliability, versatility and space are the significant benefits of the workstations in our work on Clostridium difficile“.

The Institute of Cancer Research in London is one of the world’s most influential research institutes, with an outstanding record of achievement dating back more than 100 years. At the Institute, George Poulogiannis uses a combination of Whitley i2 Instrument Workstation (with Seahorse XF Analyzer) and Whitley H35 Hypoxystation in his research into breast cancer. Hypoxia is a key factor in the “Hallmarks of Cancer” and this team are studying the role of hypoxia in cell invasion and metastasis, oncogene-induced senescence and resistance to current treatment options. The i2 and H35 replicate a physiologically relevant atmosphere for these studies, enabling consistent and reliable results. This combination of Workstations is also used by Dr Ayse Latif, who is researching gynaecological cancers at The University of Manchester.

Don Whitley Scientific would like to take this opportunity to thank all customers who have provided testimonials. If you would be interested in supplying a testimonial, please contact Alex_Rhodes@dwscientific.co.uk.

Take a look at our other testimonials

bradford-ict

Cancer Cell Study Carried Out At The University Of Bradford

A paper entitled “Plysialic acid sustains cancer cell survival and migratory capacity in a hypoxic environment” has been published by researchers at The University of Bradford and The University of Huddersfield. The study looks at Polysialic acid (a unique carbohydrate polymer expressed on the surface of neuronal cell adhesion molecules) and its association with tumour cell and adhesion in hypoxia. Their findings provide the first evidence that polySia expression sustains migratory capacity and is associated with tumour cell survival in hypoxia.

A key part of the study involved the use of a Whitley H35 Hypoxystation. The importance of using a piece of equipment such as a Whitley Hypoxystation is down to hypoxia having a profound effect on cancer cell growth as it occurs in poorly vascularised areas of tumours. Klaus Pors, Senior Lecturer In Chemical Biology, provided the quote below:

Dr Robert Falconer and colleagues are studying polysialyl transferases (polySTs), responsible for the biosynthesis of polysialic acid (polySia), as a potential antimetastatic therapeutic strategy. PolySia is a unique carbohydrate polymer capable of modulating cell-cell and cell-matrix adhesion, migration, invasion and metastasis in a number of cancers. In this study we have employed Don Whitley H35 Hypoxystation to analyse how polySia sustains cancer cell survival and migratory capacity in a hypoxic environment. We believe these results contribute significantly to our understanding of how polySia supports an aggressive phenotype and further studies are underway to underpin these findings in a therapeutic context.

Furthermore, the paper also states that the “results have significant potential implications for polyST inhibition as an anti-metastatic therapeutic strategy and for targeting hypoxic cancer cells”.

The group are set to continue this line of work, using the Whitley H35 Hypoxystation. Keep an eye on the DWS blog for more articles on this.

 

dws-stand

Don Whitley Scientific Exhibit at Key Cardiovascular Meeting

Don Whitley Scientific recently attended the BSCR (British Society of Cardiovascular Research) Annual Meeting, held this year at The University Of Leeds. The focus of the meeting was on “Cardiovascular Disease and Diabetes” and the event featured fantastic talks and poster presentations from renowned figures in Cardiovascular research.

The Don Whitley Scientific stand featured a H45 Hypoxystation. The H45 is a large, three port cell culture workstation, ideal for replicating physiologically relevant atmospheres through the control of oxygen, temperature and humidity. Working in a Hypoxystation means cell cultures and other samples are within a stable atmosphere that matches the specific microenvironments that exist inside the human body, meaning research results are dependable and accurate.

 

At The University of Liverpool, Dr Michael Cross is conducting cardiovascular research using a Whitley H35 HEPA Hypoxystation as he looks at Low Oxygen Drug Toxicity Testing in Cardiac Spheroids

The Whitley Hypoxystation range can be viewed here

Whitley H35 Hypoxystation

DWS Goes For Gold at VCCRI Meeting

Don Whitley Scientific Pty will be gold sponsors for this year’s Victor Chang 17th International Symposium at the Garvan Auditorium in Darlinghurst, Sydney. The meeting will incorporate the annual cardiac theme meeting of Stem Cells Australia and the annual meeting of The Australian Network of Cardiac and Vascular Developmental Biologists.

The 2016 event is a series of short lectures on relevant topics by leading international cardiac doctors and researchers. The programme covers a wide range of topics from basic science to clinical medicine. Some of the topics that will be discussed include heart development and congenital heart disease, genetic basis of dilated cardiomyopathy, cardiac muscle function and regeneration, stem cells and new heart failure therapies. For more information visit the VCCRI website.

The team from Don Whitley Scientific will have a trade display and will be able to discuss the wide range of Don Whitley Scientific equipment. Product demonstrations can also be arranged. The VCCRI currently houses two Whitley H35 Hypoxystations which are contributing to fantastic research projects. The Whitley Hypoxystation range offers the ability to work at specific atmospheric conditions, where the environment needs to be physiologically relevant to the levels of oxygen that would be found in the human body. These hypoxic workstations are available in different sizes with a number of options that can be applied to better suit the user’s application, such as front and side loading letterboxes that allow the user to quickly introduce samples into their working area.

The Victor Chang Cardiac Research Institute (VCCRI) was opened in 1994. Victor Chang was a surgeon, researcher and humanitarian who established the Cardiac Transplant Ward and Cardiac Diagnostic Unit at St Vincent’s Hospital in Sydney. Following his untimely death in 1991, the Victor Chang Cardiac Research Institute was established, initially under St Vincents Hospital. It became an independent research facility the following year in 1995. In 1996 it moved to its current home, the Garvan Building, where its premises were opened by the late Diana, Princess of Wales.

 

Scientist Working in Whitley Workstation

Examining the Role of Autophagy in Hypoxic Tumours

Hypoxystation users Tan et al. at the University of Toronto published a paper in June examining the significance of autophagy in cancer development (“Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors“, Neoplasia (2016) 18, 347-355). Autophagy as a means of recycling cell components is induced under stress conditions such as hypoxia, and Tan et al. investigated the correlation of hypoxia and autophagy in solid tumours in the context of resistance to cancer therapeutics.

Cells were cultured in the H35 Hypoxystation for up to 48 hours at hypoxia (0.2 %) and compared to cells grown at ambient oxygen level. Gene silencing of autophagy proteins ATG7 and BECLIN1 with shRNA resulted in decreased cell survival under hypoxia, and inhibition of autophagy with pantoprazole exacerbated the loss of viability in the knock-down cells under hypoxia, demonstrating the cyto-protective effects of these autophagy proteins. Using the Seahorse XFe Analyzer to assess oxygen consumption in wild-type and silenced cells, Dr. Tan’s lab found reduced respiration when autophagy is disrupted, possibly due to accumulation of dysfunctional mitochondria in these mutant cells. The H35 Hypoxystation  Dr. Tan’s lab used for these studies creates a closed environment with controlled temperature, humidity, CO2 and oxygen, in which cells are cultured and manipulated over the course of days and weeks without the need to transfer into ambient conditions. The combination of an Hypoxystation and an i2 Instrument Workstation is designed to accommodate the specific requirements of the Seahorse XFe Analyzer for the duration of the metabolism assays investigating oxygen consumption and extracellular acidification.

Please visit the Don Whitley Scientific website for more published papers featuring the Whitley Workstation range or contact us to discuss your hypoxia needs.

2016-05-18 15.10.56

Whitley H35 Hypoxystation used in heart regeneration project

Dr Vaibhao Janbandhu is a Postdoctoral Research Scientist at the Victor Chang Cardiac Research Institute (VCCRI) in Sydney. He has been in contact with Don Whitley Scientific to explain how his lab’s work has benefited from the use of a Whitley H35 Hypoxystation. Vaibhao uses the Hypoxystation to isolate, culture and characterise adult cardiac stem cells (CSCs).

Dr Janbandhu had already been using a H35 that was set up at the institute for almost three years before he got his own unit installed last year. Specifically, his project is to find new ways to stimulate heart regeneration during ageing and after heart attack. For this he needs a way to isolate, culture and characterise adult CSCs. In Vaibhao’s words the H35 Hypoxystation seems well suited for this application: “the DWS Hypoxystation provides a highly stabilised environment in which levels of oxygen, carbon dioxide, temperature and humidity are precisely controlled and it will be an integral part of the project to advance the project aims”.

Vaibhao explains that mammalian stem cells reside in a specialised cellular microenvironment. This niche and the stem cell niche is characterised by a low partial oxygen pressure. This hypoxic niche protects stem cells from deleterious effects of O2 on proteins and DNA. These precise conditions are essential for Dr Janbandhu’s work as they accurately replicate the in vivo environment.

His work also see benefits when comparing the use of a Hypoxystation as opposed to using a CO2 incubator. Typically, cell culture work involves methods which include isolating cells under their usual physiologically relevant conditions and then working with them in “bench-top conditions” where cells are exposed to non-physiological oxygen. This can then lead to altered hypoxic response, metabolism, reactive oxygen species and DNA damage response. This metabolic stress introduces unknown outcomes and may lead to results inconsistent with physiological processes. Therefore, the precise control of oxygen levels in cell culture has been shown to be vital for reproducible and physiologically relevant results, transforming the working environment in Vaibhao’s lab.

As well as the precise controlling of conditions, Vaibhao likes the remote access feature on his H35 Hypoxystation. The remote access allows Vaibhao to log into his Hypoxystation’s touchscreen control whilst away from the unit, offering increased flexibility in his working methods. Additionally, he likes how he can view operating conditions, set parameters and change access levels remotely.

Dr Janbandhu opted to purchase a Hypoxystation from Don Whitley Scientific for the level of service and specification we were able to provide in Australia. Other companies either couldn’t fulfil configuration requirements and didn’t provide sales and or service in Australia. Don Whitley Scientific’s office in Australia, provides both fantastic sales and service nationwide. Vaibhao also states that from “discussion with other research groups across the world we felt confident to go for a DWS Hypoxystation”.

Dr Vaibhao Janbandhu has this to say about Don Whitley Scientific Pty Ltd: “I would like to thank your company personnel at the customer services division in Australia for their excellent support. Your Sales & Service Manager in Australia, Grant Shallcross, took care of all my queries in a jiffy!”. Vaibhao added that the funding for the purchase came from the James N Kirby Foundation and the Victor Chang Cardiac Research Institute.

2016-05-18 15.13.27

IMG_0294

Don Whitley Scientific in Spain

The Don Whitley Scientific demonstration truck is currently making its way through sunny Spain! Accompanied by our sales team, the truck is visiting various research and clinical institutes throughout the country to discuss how DWS equipment may enhance and benefit a range of scientific applications.

The demonstration vehicle is becoming an increasingly useful resource, enabling DWS to visit customers around the continent to demonstrate a wide range of equipment. The truck has previously been to Europe (Poland, Czech Republic, Hungary and Austria) as well as sites across the UK and is now visiting existing customers and distributors, as well as potential new customers, in Spain.

Last week saw the trip begin, with Joe Walton and Sally Shelton setting off from Portsmouth on a ferry to Bilbao. The first destination was Pamplona, where Joe and Sally met up with Sandra Berges from our Spanish distributor, Nirco, to visit existing and potential customers in the area. The truck provided Sally and Joe with the ability to demonstrate products and have an area to  discuss any potential sales with our customers. It also allows us to obtain customer feedback on how our equipment works in their lab, in this case a Whitley H35 Hypoxystation, which the customers were actually very happy with.

The next day involved a long seven and a half hour drive to Barcelona. Once in the capital, the team visited a hospital to see current customers who are using Whitley products in various research based applications. Next stop was the research building at another  hospital in the area where visitors to the truck displayed a clear interest in the Whitley Hypoxic Workstation.

The last day of this part of the road trip involved visiting some existing customers at a research centre in Barcelona, their research involves Treponema denticola and Tannerella fosythia, which need strict anaerobic conditions to grow. The researchers at this institution have been using Don Whitley Scientific equipment for 20 years and discussed replacing one of the older models they had with one from the more modern Whitley Workstation range.

We would like to say a big thanks to Sandra from Nirco as the trip was a very worthwhile experience.

 

IMG_0290

Scientist working in Anaerobic Workstation

Liverpool Lab Highlights Significance of Hypoxia in Glioblastoma Research

Studies into the effects of hypoxia on different areas of glioblastoma were recently published by a lab at the University of Liverpool. Violaine See’s lab investigated the effect of both cell cycle progression and specific chemical signatures under varying levels of hypoxia. In both instances, the physiologically relevant environments created by Whitley H35 Hypoxystations can be considered an integral factor in ensuring the results were dependable and accurate.

The first paper, entitled “Cell cycle progression in glioblastoma cells is unaffected by pathophysiological levels of hypoxia” highlights the effects of Hypoxia on glioblastoma (brain tumour) cells. Specifically, the aim of the study was to investigate how varying oxygen levels can effect cell proliferation and survival in glioblastoma.  The results explain how solid tumours can be both hypoxic and highly proliferative and reinforce the importance of using the correct physiologically relevant oxygen tensions when investigating tumour hypoxia.

The second paper, “Use of infra-red microspectroscopy to elucidate a specific chemical signature associated with hypoxia levels found in glioblastoma” also looks at the effect differing oxygen levels can have on glioblastoma. Studying the metabolic changes that occur in tumours which are triggered by hypoxia, the paper describes the role these low oxygen levels play in the development and aggressiveness of the tumour microenvironment.

In both of these studies, the cells were cultured and incubated in a Whitley H35 Hypoxystation at a level of 1% oxygen. The H35 Hypoxystation is an ideal hypoxic cabinet for ensuring a stable, physiologically relevant atmosphere for cell cultures. Features like data logging make sure conditions can be monitored over a long period of time and the Hypoxystation is fitted with an oxygen sensor to make sure a consistent atmosphere is maintained within the cabinet.

This is not the first time Violaine See has mentioned the use of the Whitley Hypoxystation in her lab. She has provided a video testimonial for Don Whitley Scientific as someone who is happy with her workstation and the level of service provided.


 

Whitley H35 Hypoxystation

TRACER mimics oxygen and nutrient gradients in tumours

Our US/Canada distributor, HypOxygen, wanted to find out more about Whitley H35 and H45 Hypoxystation customer Brad Wouters’ recent research, especially the newly published paper describing his three-dimensional tumour TRACER project, published as “A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients“ (Rodenhizer et al., Nature Materials 15, 227–234, 2016).

Interview with Brad Wouters, Princess Margaret Cancer Center, Toronto.  March 4, 2016

“The paper describes a new device that enables us to create naturally occurring oxygen gradients, such as the ones found in tumours. The TRACER (engineered tumour roll for analysis of cellular environment and response) developed by Alison McGuigan of the University of Toronto is basically an engineered tumour that is assembled by rolling a single-component biocomposite sheet. Respiration-induced oxygen and metabolite gradients are established inside the device, so it’s an alternative to other 3D models such as spheroids. The benefit is that the TRACER rolls can be rapidly disassembled, enabling you to interrogate the biological phenotypes or properties, characteristics, and metabolites of the cells in those defined locations within the gradient, in the six layers of the tracer. The cells can be removed in a variety of ways; you can remove the cells in a viable way and process them as you would any cells, to do flow cytometry, metabolomics, cell survival, proliferation.

Read more