DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


Contact Us +44 (0) 1274 595728 sales@dwscientific.co.uk

Follow Don Whitley Scientific

Posts Tagged ‘Anaerobic Bacteriology’

Calibrating a Workstation to UKAS standards

UKAS Calibration and Temperature Uniformity in Whitley Workstations

ISO 15189:2012 Medical Laboratories- Requirements for Quality and Competence requires medical laboratories to have documented procedures for the calibration of equipment that directly or indirectly affects examination results. Equipment calibration is also beneficial for laboratories  without specific accreditation. For example in research institutions it demonstrates compliance with internationally recognised quality standards and increases confidence in test results. This principle is particularly applicable to temperature controlled equipment, such as incubators and anaerobic workstations, which may be used during the isolation, enumeration or identification of bacteria.

Benefits

The benefits of temperature calibration, and temperature homogeneity throughout the working volume of an appliance, are well understood for conventional incubators because variability in performance can affect the numbers, sizes and other visual characteristics of bacterial colonies growing on agar plates, as well as bacterial growth rates in liquid culture. Microbiologists are also beginning to appreciate the influence of temperature and other environmental parameters on the growth and viability of anaerobic bacteria.

Fastidious Anaerobes

An increasing number of our customers use anaerobic workstations to grow highly fastidious, poorly characterized anaerobes, which have been identified in complex ecosystems using molecular techniques but, until recently, have not been cultured. For example, there is considerable current interest in the bacterial species which constitute the normal human gut microbiota and the role of these organisms in human health. The growth requirements for many of these species are not yet fully understood. Ensuring temperature stability and homogeneity throughout the working area of an anaerobic chamber provides an additional level of assurance when working with fastidious anaerobes and removes a potential variable when attempting to optimize their growth and study their physiology.

Temperature Mapping

If your laboratory must demonstrate that temperature performance could directly or indirectly affect results, the temperature mapping process provides independent, UKAS-accredited testing and supporting certification. It confirms that the workstation temperature is controlled and displayed within specified tolerances. This process comprises calibration of the workstation temperature sensor and a comprehensive temperature profile of the entire working and incubation area. Data is collected from up to 12 sensors, traceable to national standards, positioned throughout the workstation chamber.

How we achieve a uniform temperature gradient in Whitley Workstations?

This is achieved through careful design of the product, optimising air flow and temperature control to achieve the lowest possible temperature gradient. Don Whitley Scientific has been designing and manufacturing modified atmosphere workstations for over 40 years so have a wealth of experience in this area.

DWS UKAS Service

In order to save you the cost of expensive call outs and project delays, we can often provide essential maintenance, schedule routine servicing and carry out your UKAS calibration/ validation work in the same visit. Whilst onsite, our engineers will not only take the time to explain the calibration procedure and why it is necessary but they will endeavour to provide your certification within 5 working days. This helps to make sure the whole UKAS process is as painless as possible for you and your staff.

Even if your laboratory doesn’t necessarily need UKAS accreditation, the cost is comparable with that of standard calibration. This means that for added peace of mind, UKAS calibration/validation could be a very shrewd investment. And because we can carry out any repairs or servicing at the same time, this can help avoid the cost of additional call outs and reduce down-time in your laboratory.

The technical expertise employed within Don Whitley Scientific for designing and manufacturing our own microbiology equipment provides an ideal background for our UKAS-approved calibration engineers.

Your queries answered – no obligation

If you have any additional questions about UKAS or would like any additional information about the benefits that this service could bring to your laboratory, please contact us today for a no-obligation consultation.

T: 01274 595728  E: sales@dwscientific.co.uk  W: www.dwscientific.co.uk

 

What is UKAS?

UKAS (United Kingdom Accreditation Service) is the sole accreditation body recognised by government to assess organisations that provide reliable data and results for a defined set of tests. All assessments are made against internationally recognised standards.

DWS Accreditation

DWS is accredited to BS EN ISO/IEC 17025:2005 for calibration activities. This means that we have been assessed by UKAS and are able to provide calibration and validation services for heat sterilisation equipment, temperature controlled processes and temperature indicators.

Picture of Helen Inns in The Mould That Changed the World

The Mould That Changed the World

Don Whitley Scientific has some very talented customers, not least Leeds Biomedical Scientist, Helen Inns. Helen is usually to be found at the WASPLab, working with bacteria including MRSA and Carbapenemase-Producing Enterobacteriaceae. This month, instead of just looking for “Superbugs”, she’s making a song and dance about them – at the Edinburgh Fringe in a show called The Mould That Changed The World.

Helen is part of a chorus of healthcare professionals performing in the world premiere of a brand-new musical, called The Mould That Changed the World. The show is a fun educational production which tells the story of Fleming’s discovery of penicillin, the development of antibiotics, and the looming crisis of antimicrobial resistance which now threatens healthcare as we know it.

Banner ad for The Mould that Changed the World

The Mould That Changed the World is receiving rave reviews at the Edinburgh Fringe, where it’s showing daily until 25th August.

After that, a free resource pack will be available, so primary schools can put on their own production and empower children to learn about and spread the message of antimicrobial stewardship.

Follow on Facebook and Twitter:  https://www.facebook.com/mouldchangedtheworld/

https://twitter.com/ThatMould

Find out more, or sign up for the schools production, at www.mouldthatchangedtheworld.com

 

 

wasp_lab_to be at Microbe 2018

Visit Don Whitley Scientific at Microbe 2018 and win a prize

 

Are you booked to attend this year’s Microbe in Sheffield? If you are, call at the Don Whitley Scientific stand to see WASPLab, ChromaZona and Whitley Workstations. There are also prizes to be won!

 

WASPLab

At what promises to be another entertaining, interesting, informative event, Don Whitley Scientific will showcase WASPLab. This is the complete robotic specimen processing system – including versatile software that conducts multiple plate scans during incubation, enabling early detection of positive growth. It provides the ability to perform antibiotic sensitivity testing; sub-culture work; and links to the MALDI-TOF identification system.

CHROMAZONA Automatic Colony Counter


ChromaZona

We will also be showing ChromaZona, the automated microbial identification and AST analysis system for faster results – and to EUCAST standards – in  busy clinical laboratories.

Whitley Workstations

Whitley A35 Anaerobic Workstation

Whitley A35 Workstation

If you need ideal conditions for the processing, incubation and examination of anaerobes and microaerophiles, come and discover the range of Whitley Workstations and the new Catalyst Monitoring System.

For more information

And if you are not able to attend Microbe this year but would like information on any of these products (or to discuss others in the Whitley range), please visit our website at www.dwscientific.co.uk or contact our sales office on sales@dwscientific.co.uk or call 01274 595728.

 

 

PaCMAn Course Photo

Successful PaCMAn Course 2018

Another successful Practical and Clinical Microbiology of Anaerobes course was held on 14 & 15 June in Cardiff. We had a full complement of 26 delegates, including overseas visitors from Ireland, Norway, Sweden, Romania, Germany and Costa Rica.

This 2-day residential course enables delegates to refresh their knowledge on culturing, identification and the clinical importance of anaerobes and allows them to learn from clinical and laboratory professionals about the latest technologies and techniques. The course is delivered by the UK Anaerobe Reference Unit, Public Health Wales, Cardiff and is CPD accreditated by RCPath (11 credits).

 

The main aims of the course are:

·         To promote an understanding and awareness of anaerobic bacteria in clinical material.

·         To gain an insight into their relevance in clinical microbiology.

·         To achieve a basic level of competence in methods used for their isolation and identification.

·         To improve the standards of anaerobic microbiology in clinical laboratories.

 

Feedback from delegates has again been very positive with the majority rating it ‘excellent’ overall. Participants particularly enjoyed the friendly and informative course atmosphere and provided comments such as:

“Excellent course will definitely recommend to others.”

“Will be recommending for colleagues to attend.”

“Engaging speakers, very helpful, great team. I would highly recommend this course. I have learnt so much and look forward to passing all this great information to my team at work.”

“A fantastic course that came highly recommended to me and I will certainly
recommend to others.”

“It is an excellent workshop and I will recommend the course, even if you are working outside of the UK. Lectures were clear and well organized. The booklets are very educational for my needs.”

 

Whitley A55 WorkstationDon Whitley Scientific sponsors and helps to administer the course on behalf of the UK Anaerobe Reference Unit (ARU). We loaned the ARU a Whitley A55 Workstation for the course so that they had increased incubation capacity for the practical sessions.

It is planned to hold the course again in 2019 but dates have not yet been finalised. Keep an eye on the DWS blog or contact us on sales@dwscientific.co.uk if you are interested in attending this event.

Biomedical Scientist_Clinical Microbiology of Anaerobes Course

Practical and Clinical Microbiology of Anaerobes Course 2017

The Society for Anaerobic Microbiology proudly presents …

A 2 day residential course delivered by the UK Anaerobe Reference Unit, Public Health Wales, Cardiff

15-16 June 2017

Is it time to refresh your knowledge on culturing, identification and the clinical importance of  anaerobes?

Are your staff wanting to learn from experts about the latest technologies and techniques?
Only 20 places available

CPD accreditated by RCPath (11 credits)

Invaluable preparation for FRCPath

Cost: £350 + VAT (£330 + VAT for SAM members)

Cost includes one night accommodation at the Park Plaza Hotel, Cardiff and all meals & refreshments plus dinner on 15th June.

Aims of the Practical and Clinical Microbiology of Anaerobes Course

To promote an understanding and awareness of anaerobic bacteria in clinical material.
To gain an insight into their relevance in clinical microbiology.
To achieve a basic level of competence in methods used for their isolation and identification.
To improve the standards of anaerobic microbiology in clinical laboratories.

Preliminary Programme

Thursday 15 June

09:15 – 09:45 – Registration and Coffee
09:45 – 10:00 – Welcome and Introduction
10:00 – 12:30 – Session 1 – Back to basics – ID, susceptibility testing, hot topics
12:30 – 13:30 – Lunch
14:00 – 17:00 – Practical Laboratory Session
19:30 for 20:00 – Dinner and Quiz

Friday 16 June

09:00 – 12:30 – Clinical Case Histories
12:30 – 13:30 – Lunch
14:00 – 16:00 – Practical Laboratory Session
16:00 – 16:30 – Course Review and Departure
To reserve your place on the Practical and Clinical Microbiology of Anaerobes 2017 course, please contact:
Deborah Robinson at Don Whitley Scientific Limited on 01274 595728 or Email sales@dwscientific.co.uk

Places will be allocated on a first come, first served basis.

Payment accepted by cheque (please make payable to Don Whitley Scientific Limited)
and Visa or Mastercard.

Sponsored by Don Whitley Scientific Limited

Clostridium difficile studies can be done in a Whitley Workstation

Whitley Workstation used in Clostridium difficile study

The Whitley A35 Workstation has recently been used by a group at Monash University in a study relating to toxins affected by Clostridium difficile.

This very interesting research paper, entitled “CdtR Regulates TcdA and TcdB Production in Clostridium difficile“, was written by researchers at Monash University in Melbourne, Australia. The results from the study “establish CdtR as an important virulence regulator in two clinically important, epidemic strains of C. difficile, and further highlights the need to investigate regulatory mechanisms of important virulence factors in diverse strain backgrounds.”  The paper proves for the first time that “TcdA and TcdB production is linked to the production of CDT by a common regulatory mechanism”.

Click here to read the full paper

WJGS Video th

New Video: Whitley Jar Gassing System Animation

Don Whitley Scientific have released a new video, an animation highlighting the effectiveness of using a Whitley Jar Gassing System to create anaerobic and microaerobic conditions.

The animation shows key features that make the Whitley Jar Gassing System a fast, low cost solution for creating specific atmospheric conditions whilst being a convenient and easy piece of equipment to use. Using the clear touchscreen display, the user can create anaerobic conditions within 2 minutes and microaerobic conditions in a rapid 15 second cycle. These cycles are displayed as graphs on the touchscreen and then available to print out for traceability and accreditation purposes.

The WJGS is part of a wide range of Don Whitley Scientific products that provide efficient and reliable conditions for a range of microbiology applications. When released the Whitley Jar Gassing System was an innovative new way to create anaerobic/microaerobic conditions and it has evolved with technology, continuing to provide a low cost solution to create the relevant atmosphere for working with anaerobes and microaerophiles.


 

Introduction to Clinical Anaerobic Bacteriology

Free Guide to Clinical Anaerobes

Need to identify clinical anaerobes?

Would a free guide be of use to you?

An Introduction to Clinical Anaerobic Bacteriology is a unique guide that enables readers to isolate and identify 12 commonly occurring clinically important anaerobic bacteria. It consists of 60 pages and is illustrated with stunningly detailed colour photographs and attractive reference tables.

Designed to fill a gap in the practical reference materials currently available to support clinical laboratory practice for microbiologists, the publication was written by Professor Michael W D Wren, MBE FIBMS, former consultant Biomedical Scientist in the microbiology department, University College Hospital, and visiting Professor at The University of Westminster. With a foreword by Professor Brian Duerden, Emeritus Professor of Medical Microbiology at Cardiff University and input from Dr Don Whitley, Chairman and founder of Don Whitley Scientific Limited, considerable expert knowledge has been combined to make this the most up-to-date reference guide for the modern clinical laboratory.

Images were kindly supplied by the Anaerobe Reference Unit, Cardiff.

For a limited time only (until stocks last) we are offering a free copy of this publication to those who would find it useful in their work. To apply for your free copy, please contact us at sales@dwscientific.co.uk or complete our online contact form. This offer is only available to customers in the UK.

The publication is also available to purchase as a spiral bound edition, printed on waterproof, tear-resistant synthetic paper to withstand regular use at the bench. For more information, please see our website or contact us on sales@dwscientific.co.uk.

 

New Poster from Liverpool University

Researchers from the University of Liverpool (Department of Infection Biology, Institute of Infection & Global Health and School of Veterinary Science), have produced a poster entitled “Understanding the aetiology and infection reservoirs of digital dermatitis in beef cattle and sheep”.

As part of their work, they cultured treponemes in a Whitley A35 Anaerobic Workstation.

Whitley A35 Anaerobic Workstation

Whitley A35 Anaerobic Workstation

Abstract:

Digital dermatitis (DD) is an infectious hoof disease causing severe lameness in ruminants worldwide. The primary cause has been identified as the spirochaetal bacteria, treponemes. A comprehensive bacterial molecular survey of sheep contagious ovine digital dermatitis (CODD) lesions has not been done to determine if there is a shared etiopathogenesis between BDD and CODD. Also, there have been only anecdotal reports of BDD lesions occurring in beef cattle, with no definitive published data on the disease.  Additionally, little is known about the transmission of Treponema bacteria. It may be possible that DD treponemes are carried in both cattle and sheep gastrointestinal (GI) tracts and then shed in faeces and spread. Another route of transmission may be via farm equipment.

This study investigated ruminant GI tissues and farm equipment for the presence of DD treponemes. Additionally, sheep and beef DD lesions were analysed for the presence of DD treponemes by PCR and culture techniques.

The full poster can be viewed  here.

Protos 3 Colony Counter

New Protos 3 Colony Counter

New Protos 3 colony counter and rapid ID system saves time and provides accurate, traceable results in microbiology

Don Whitley Scientific Limited introduces a vibrant new automated colony counting and chromogenic identification system, Protos 3. This system allows accurate and reproducible identification of colonies cultured on chromogenic plates.

What makes the new Protos 3 outperform other commercial colony counters is the system’s ability to count colonies in seconds and identify microbial species by their colour on chromogenic plates. This is a great time saver, providing accurate, objective and fully traceable GLP compliant results.

The stylish, yet practical Protos 3, which comes in bright red, attaches easily to a computer and requires minimal training to set up. Users simply input their plate identification and click. The Protos 3, featuring a highly sensitive CCD camera combined with unique three colour LED lighting, rapidly images an infinite number of colony colours on one plate and detects colonies as small as 0.043mm.

The Protos 3’s powerful software then generates true to life counts and plate images, which can be transferred and stored in Excel. This GLP compliant process, with its full audit trail eliminates keying and image transfer errors providing accurate, objective data, which can be reviewed anywhere and anytime.

“Microbiologists have always wanted a colony counter that combines throughput and automatic colony ID,” commented Steve Robertson, Sales Director. “The new Protos 3 offers a high performance platform that generates fully traceable data and we know it will be a huge time saving device for microbiologists in food and pharmaceutical laboratories. For anyone using a spiral plater for carrying out multiple bacterial enumerations, this is a perfect solution.”