DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Hypoxystations

Whitley i2 Instrument Workstation in use

Whitley Workstations Used in Novel Research Approach to Neurodegenerative Disorders

Efficient energy production is primarily driven by oxygen and is crucial in the brain, which consumes 20% of the body’s total energy whilst weighing only 2% of the total body weight. Loss of oxygen in the brain (hypoxia) can result in brain damage, which can contribute to dementia including Alzheimer’s disease (AD) and can influence amyotrophic lateral sclerosis (ALS, commonly known as motor neuron disease) disease mechanisms. Those mechanisms are often poorly understood, however, the cellular consequence of hypoxia including disruptions to energy supply are often observed in the brains of patients with these neurodegenerative disorders. The ability to model a hypoxic environment and study the effects on AD and ALS in the laboratory has historically been limited by the lack of available technology.

The SITraN laboratory takes the gold standard approach for measuring the effect of hypoxia on energy generating pathways in cells by using an XF metabolic bioanalyser (which measures energy generation in a non-invasive manner in cells, Seahorse Bioscience/Agilent), housed within an i2 Instrument Workstation and H35 HEPA Hypoxystation. The i2 chamber has been designed specifically to house and run metabolic flux assays using the XF bioanalyser in a hypoxic environment. The H35 section of the chamber is HEPA filtered and gas, temperature and humidity controlled allowing us to perform cell manipulation on our ALS and AD cell models.

Using this novel combination of technologies – SITraN aims to measure how hypoxia affects the energy generating pathways in brain cells. Their novel approach will allow the identification of the pathways involved in the cell response to hypoxia, which lead to energy disruption in patients with AD and ALS and will allow therapeutic hypoxic markers of disease to be identified for future clinical studies.

Article provided by Dr Scott Allen, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield. 

Dr Allen will be presenting at the ALS/MND Symposium in Glasgow held from 7-9 December 2018.

Range of Large Hypoxic Workstations to Accommodate Laboratory Instruments

Abrupt changes in temperature and oxygen levels can significantly alter the many oxygen-sensitive signalling pathways that sense and react to the cellular micro-environment. Don Whitley Scientific have developed a range of hypoxic workstations large enough to accommodate many instruments which would previously have been confined to the bench; minimising the need to transfer cells between bench and workstation which would result in exposing them to raised levels of oxygen.

The H135 HEPA Hypoxystation and i2 Instrument Workstation have larger footprints and optional shelving to provide ample space for both instrumentation and culturing. The removable front facilitates transfer of equipment in and out of the workstations. Precise control of temperature, oxygen, carbon dioxide and humidity mimic the physiological environment, ensuring true in vivo cell behaviour.

Working with manufacturers of diverse laboratory instruments, Don Whitley Scientific have validated a number of options for analysis and imaging of cells:

Whitley H135 HEPA Hypoxystation

* Incucyte® live-cell analysis: the Incucyte ZOOM comfortably fits inside the H135, enabling real-time quantitative study of growing cells inside the hypoxic incubator environment

* Microscopes: a range of microscopes can be placed inside the controlled workstation environment, allowing users to image cells during incubation

* Seahorse XFe Analyzer: a modified version of the H135, the i2 Instrument Workstation, has been customised to accommodate the specific requirements of the XF Analyzer for metabolism assays.

We are happy to discuss your unique sizing and environmental needs; please contact us today to discuss any custom requirements.

What our users are saying:

“Due to the utility of having the Incucyte S3 live imaging system inside our H135 Workstation, we have expanded our collaboration portfolio exponentially. We have performed cancer biology, immunology, neuro and vascular physiology, and many other types of experiments in our system under controlled atmospheric conditions. The large volume of the H135 is key to having enough room for both the imager and sufficient workspace to carry out experiments.”

– Dr Adam Case, Assistant Professor, Dept. of Cellular and Integrative Physiology,
University of Nebraska Medical Center, Omaha, NE.

 

Distributor Meeting Held at New Factory

Last week, DWS welcomed 38 representatives from over 20 different countries for the first International Distributor Meeting to be held at our new factory in Bingley. On the first night we held a welcome dinner, and as part of the evening there was a presentation to four of our distributors who had excelled in certain areas. Our special thanks and congratulations to VWR (Sweden), Lab Technologies (India), Cold Spring Biotech Corp (Taiwan) and Hua Yue Enterprise Holdings Ltd (China). Another award should go to our colleagues from AS1 Limited in New Zealand, who travelled over 11,000 miles to be with us!

It was a busy few days packed with sales and installation training on the WASP Touch and our hypoxic, anaerobic and microaerobic workstations. The meeting provided an invaluable opportunity for the distributors to get hands-on experience with our whole product range and to receive training from the people who design and build our equipment on a daily basis. One of the attendees commented: “Our thanks to you and all your colleagues for a brilliant couple of days and a superbly structured meeting which I’m sure will be very fruitful.”

As with our past meetings, we felt it was important to balance out the hard work with some fun and offer our guests a chance to socialise after the training sessions. Our evening entertainment included lots of delicious food and activities such as bowling, cheese-tasting, a magic show (courtesy of our wonderfully multi-talented sales rep, Stuart) and a musical performance from a local brass band.

Distributors_Oct18_Group PhotoWe are very grateful to the distributors who took time out of their busy schedules to attend, and for all of their hard work during the week. We hope everyone enjoyed it as much as we did and look forward to our next meeting!

Hollins_Oct18_Award_Labtech India

 

 

 

 

 

 

 

 

2018 DWS Microbiology Prize Awarded

Earlier this year, we presented the annual Don Whitley Scientific Prize for Microbiology to the student who achieved the highest marks in Microbiology at the University of Bradford. The 2018 winner, Christiana Victoria Cismaru, gained a 1st Class Honours and said of her experience:

“I am honoured… this is a huge accomplishment for me, a reward for all the hard work and time I invested whilst at the University of Bradford. There was so much to learn… Choosing Medical Microbiology as my final year option allowed me to focus on the subjects I was most interested in and opened my eyes to current issues, such as emerging viruses and antimicrobial resistance; this has influenced me in choosing to pursue a research career in virology after graduation.”

Christiana will be going on to study for an MSc in Molecular Biology and Pathology of Viruses at Imperial College London and then hopes to pursue a PhD in the same research area; we wish her all the best in her future endeavours. She is pictured here with Dr Andrew Pridmore, Head of Science at Don Whitley Scientific and himself a recipient of the accolade in 1991.

Whitley Hypoxystation

The Leukemic Stem Cell Niche: Adaptation to “Hypoxia” Versus Oncogene Addiction

Hematopoietic stem cells (HSC) are responsible for constantly maintaining and replenishing the supply of new blood and immune cells. They give rise to both lymphoid and myeloid progenitor cells, which then proceed to differentiate down their respective paths to form various specialized cells such as erythrocytes, macrophages, B and T cells, to name a few. Within the body, HSCs are found to reside in extremely low oxygen environments called stem cell niches (SCN). Like all other regulated cell cycles, HSCs can lead to cancers such as leukemia and lymphoma if cell division becomes uncontrolled.

MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

Figure 2: Suppression of oncogenic proteins driving non-CML blood neoplasias in the course of cell “adaptation to hypoxia.” MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

In this paper, Cheloni et al. primarily focused on chronic myeloid leukemia (CML).

The authors hypothesized that suppression of the BCR-Abl oncogene is likely a key positive regulator of LSC survival within “hypoxic” SCNs. To analyse the various mechanisms and responses that CML cells demonstrate within SCNs, the authors studied correlations between varied oxygen and glucose concentrations with the amount of BCR-Abl produced. All tests were performed using two human CML cell lines, K562 and KCL22. Testing conditions were precisely controlled to mimic the SCN environment as close as possible. Using a Hypoxystation supplied by Don Whitely Scientific, a water-saturated atmosphere comprising of 0.1% O2, 94.9% N2 and 5% CO2 was generated and maintained.

The research conducted and documented by Cheloni et al. has provided great insight into several key regulatory mechanisms associated with leukemic stem cells as well as an explanation for their notorious reputation for having high relapse rates. Additionally, they established that the triggering of oncogene suppression associated with CML is due to severe energy restriction rather than simply the “adaptation to hypoxia.”

The Don Whitley Scientific Hypoxystation provides the user incredible flexibility as it can control oxygen down to 0.1% while providing a temperature and humidity controlled environment with ample working space. The extreme precision provided by the Hypoxystation was critical to the collection of accurate and reliable data when recreating the demanding environment that comprises SCNs. The atmosphere is constantly monitored and adjusted by the real-time feedback system to ensure accuracy and ISO class 3 clean room HEPA filtration is also available for long term cell culture applications.

Choose your atmosphere with the Hypoxystation hypoxia chamber. Accurately control O2, CO2, Temperature and Humidity.   

Hypoxystation is the only hypoxic chamber purpose built for physiological cell culture research. Specifically designed to create normoxic, hypoxic and anoxic conditions within a controlled and sustained workstation environment, this hypoxic incubator is ideal for research requiring the ability to accurately control O2, CO2, temperature and humidity. The Whitley Internal HEPA Filtration System provides a particle-free internal environment that exceeds ISO 14644 class 3 clean atmosphere.  With such accurate control and the ability to manipulate cells in situ without altering the incubation environment, research into cell biology can be performed over a comprehensive range of oxygen tensions with precision. Don Whitley Scientific offer the following range of Hypoxystations:

                                                                                   

Hypoxystation H35

Hypoxystation H45

Hypoxystation H85

Hypoxystation H135

i2 Instrument Workstation

Application possibilities for Hypoxystation are endless; it is being used for research into tumour microenvironment, hypoxia pathways and HIF signalling, in vitro modelling of in situ environments, cancer cell metastasis, angiogenesis, and many other fields where cells may benefit from a more physiological atmosphere.

 

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Supporting the Scientific Community

As well as manufacturing and supplying equipment for microbiology and cell culture applications worldwide, Don Whitley Scientific also makes an extensive effort to support the scientific community. For several years we have supported science through various means, these are listed below.

 

 

 

Workstation Poster Grant

If you have used a Whitley Workstation or Hypoxystation in your work and have mentioned it in your poster or published paper, let us know and you could be entitled to a grant of £250. All we ask in return is a copy of your poster/paper so we can use it on our website to help promote the range to others. Payment will be made in good faith after receipt and approval of the draft poster/paper to enable you to travel to the event to present it.

To apply for this grant, please send a copy of your draft poster or paper along with your contact details to steve_robertson@dwscientific.co.uk. If you are presenting a poster at an event, don’t forget to let us know the title of the event and the date you will be making your presentation.

 

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More Milestones Reached at Victoria Works

Don Whitley Scientific recently moved into new premises in Bingley, West Yorkshire. As expected, the move was completed in an efficient manner that has enabled the majority of our work to continue without interruption.

 

 

 

 

First workstations to leave VW

The first example of this is our first set of Whitley Workstations have been manufactured, tested and dispatched from our new workplace. On Monday, 2nd July, these first cabinets manufactured at our new premises went on final test. Only two weeks after moving to Bingley, these three DG250s are almost ready to be shipped to customers: two to Hua Yue in China and one to The University of Leeds. As mentioned this required a great deal of hard work from all involved to make sure Victoria Works was ready in time to get products out the door so quickly.

 

 

 

 

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A short while after most people started working at Victoria Works, it was time to get the specialist hauliers in to move the CNC machine shop department to Bingley. This was a large scale operation given the size and scale of the machines used by the department. However the move was managed well and they are now up and running in their new area, which offers a lot more space compared to what was available in Shipley.

 

 

 

 

 

Don Whitley Scientific has already welcomed various visitors to the new premises, where a new showroom is used to provide visitors with the experience of using DWS products first-hand. Tours of the premises are provided to give visitors a chance to see how the different departments work together to design, manufacture and service a comprehensive product range.

 

 

 

 

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Don Whitley Scientific opens new doors to visitors

With Don Whitley Scientific now firmly settled in its new home, Victoria Works, Bingley, visitors are now invited to a new showroom. This fabulous area boasts a permanent display of a wide range of Whitley products. Recently, a visitor from an increasingly relevant research topic in microbiology was the first visitor to the new premises.

Over the years, DWS invited many visitors to the old premises in nearby Shipley, from where the business ran successfully for over 30 years. However there were limitations on what products could be demonstrated due to the size of the old showroom. At Victoria Works the showroom is the ideal place for customers to come and really experience our range of workstations. You can try out some of the different porthole systems to see which would be the most suitable for your laboratory or look at the different options for size of airlock. We can sit down with you and discuss your application, your projects, what equipment you might want to use inside the chamber, the challenges you face, and what you need to achieve from the purchase of a Whitley Workstation.

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The Whitley i2 Instrument Workstation – Designed to accommodate Seahorse Extracellular Flux (XF) Analyzers in relevant conditions

Having a laboratory at Bingley provides additional benefits in that we have both microbiology and tissue culture experts on hand to give expert advice if and when required. We also have people who design and manufacture our workstations so if there is a slight modification or additional technical information required, we can get our R&D staff involved to discuss the possibilities.

 

 

The larger showroom now provides the option for bespoke areas to be created that reflect how different products may be used together in a lab environment. For example, sample preparation equipment (Smasher and Dilumat), spiral platers (Whitley WASP Touch) and colony counters (Protocol3). The size of the showroom also provides the option to display previous incarnations of DWS products, showcasing the evolution of products made possible through the work of our research and development team. For example you can see the evolution of Whitley Spiral Platers, with spiral plating being a process DWS helped establish and introduce to the world of microbiology.

 

 

 

 

 

 

Another way to view products is also provided by our 65″ screen. This offers the facility to showcase products, options and features with accompanying information and videos.

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The Whitley Interactive Product Presentation


If you are considering a Don Whitley Scientific product for your work, please don’t hesitate to get in touch to arrange a visit. We can tailor-make the visit based on your interests and ensure you are provided with understanding, knowledge and experience of DWS products. We can also give you a tour of the premises where you can see how all the different departments combine to create the products in the Don Whitley Scientific range.

Email sales@dwscientific.co.uk or give us a call on +44 (0)1274 595728


 

Whitley A95 Workstation for Jin Sung on final test

End of an Era for Don Whitley Scientific

Production comes to a close in Shipley

The last Whitley Workstation to be manufactured by Don Whitley Scientific in Shipley has now rolled off the production line. This particular order was an A95 Anaerobic Workstation ordered by Jin Sung Unitech, Republic of Korea.

JS Unitech LogoAccording to Junghoon Hong of Jin Sung, “We had no idea that in placing this order, we would be commissioning the last ever workstation to be built at Don Whitley Scientific in Shipley. We have been distributing Whitley Workstations for 19 years and are very proud to have ordered the last one from the Shipley production line.”

Paul Walton added, “I am extremely happy that we are moving to bigger and better premises that will provide the opportunity to improve our production processes. Victoria Works will provide considerably more warehousing capacity plus new offices, a bespoke production area, service centre, R&D mezzanine and improved staff facilities including a large, fully fitted, air-conditioned canteen. There will also be a new product showroom where customers can view demonstrations of the latest anaerobic and hypoxic workstation technology.

“An element of nostalgia is, however, creeping in as we draw close to the move date. We have been manufacturing in  Shipley for over 40 years and I have seen many changes in those four decades. We will, however, focus on the positives and move the business forward to face the challenges of the next 40 years!”

When Don Whitley Scientific began making workstations, it was purely for the UK market. We now have over 4,000 workstations in over 50 countries.

It might be the end of one era but another, exciting one begins at our new premises in Bingley.

Clostridium difficile studies can be done in a Whitley Workstation

An exciting opportunity to join our sales team

Anyone interested in an exciting opportunity to move into sales?

Don Whitley Scientific now has a vacancy for a Technical Sales Representative.

The Role

As a Technical Sales Representative you will promote an exciting range of laboratory equipment and service contracts. Brands include Whitley Anaerobic, Hypoxic and Microaerobic Workstations, Whitley Automated Spiral Plater plus products such as the automated specimen processor WASPLab, media/sample preparation equipment and other niche laboratory equipment.

This role will provide an opportunity for the successful candidate to further develop an established customer base and build a long-term, lucrative career in the UK as well as the possibility of overseas travel.

Candidates with no previous sales experience will be considered as training will be provided. A high level of support will also be available from senior colleagues.

 

Core responsibilities include:

  • Maximising sales potential and business growth by establishing relationships and collaborations with customers.
  • Working closely with marketing to ensure relevant promotions are delivered to customers and that feedback from customers is relayed back to inform future planning.

Essential Requirements:

  • Highly motivated and ambitious.
  • Hands-on approach and comfortable with daily travel.
  • Articulate, confident communicator able to engage with people at all levels, including technical managers and procurement/finance personnel.
  • Independent and decisive, using creative thought and sound logic to identify, prioritise and maximise opportunities.
  • Based in the north of England to serve a territory that extends into Scotland.

Our sales team comprises of successful people with and without a science background, so experience in a scientific discipline is not a prerequisite.

 

Remuneration

Package includes:

  • Basic salary dependent upon experience
  • Attractive bonus scheme
  • Company car

How to apply

Please email Steve Robertson, Sales Director on steve_robertson@dwscientific.co.uk attaching a current CV.

STRICTLY NO AGENCIES