DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Case Study

hepa filter

HEPA Filtration from Don Whitley Scientific

For over forty years, Don Whitley Scientific have utilised experience and expertise to ensure products make working methods more straight forward for our laboratory customers. With more and more importance placed on clean air, HEPA filtration can provide an extra measure to ensure research carried out within Whitley Workstations is done so in an environment that achieves particulate concentrations exceeding the levels detailed in ISO 14644-1 Part 1, Table 1, (Class 3) Classification of Air Cleanliness.

 

Tests have been carried out by Don Whitley Scientific that show just how much of a difference a HEPA filtration system makes to the atmosphere of a Whitley Workstation. Don Whitley Scientific have been manufacturing products for over 40 years and the introduction of HEPA filtration was a logical step to make sure our customers have an option that falls in line with the latest laboratory trends and requirements.

The benefits of the Whitley HEPA filtration system are:

  • The Whitley system utilises an internal HEPA filter. The atmosphere is cleaned quickly and continuously because the entire internal atmospheric volume of every Whitley Workstation passes through the filter hundreds of times an hour.
  • The atmosphere passing through the filter creates a laminar flow-like effect covering around 94% of the width of the chamber.
  • The location of the Whitley HEPA filter inside the workstation ensures it cannot get wet. Therefore it cannot become saturated with moisture and rendered ineffective.

Don Whitley Scientific has worked with one of the world’s leading filter manufacturers to develop a filter specifically tailored to suit the requirements of anaerobic workstations. Adding HEPA filter technology to the workstation means creating an environment where microbiology samples and cellular cultures can grow free from the risk of contamination.

HEPA filtration was introduced as an option after decades of experience in microbiology. We are confident that our thorough approach to product development and product testing has resulted in a HEPA filtration system superior to any other systems fitted to positive pressure modified atmosphere workstations. The video below explains Whitley HEPA filtration in further detail.

 

 


Explore the Whitley HEPA Workstation range


 

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How to choose an Anaerobic Workstation

In this exclusive interview with rapidmicrobiology.com, Dr. Andrew Pridmore, Head of Microbiology at Don Whitley Scientific, discusses the benefits of using an anaerobic workstation over traditional gas jars. Andrew explains what to look for when choosing a system and how the new Whitley A135 HEPA can deliver efficient, cost effective anaerobic incubation conditions for routine and research laboratories.

What are the benefits of using a modified atmosphere workstation over gas jars?
Anaerobic jars can be prone to leaks and, therefore, may not always provide strictly anaerobic conditions. They also require a large volume of incubator space and become expensive to operate in laboratories with a high anaerobic workload. Most importantly, culture plates in anaerobic jars cannot be inspected during incubation; anaerobic conditions must be interrupted to read the plates. This results in a loss of bacterial viability if plates need to be reincubated or when subcultures need to be made for further investigation.

In comparison with jars, anaerobic workstations offer more precise and reliable atmospheric control, more stringent anaerobic conditions and consistent low running costs. Perhaps their greatest advantage is that culture plates can be inspected at any time without disturbing the incubation conditions. In clinical laboratories, this facility has been proven to increase the isolation rate of anaerobic pathogens and also provides the earliest possible indication of their presence in a patient’s sample.

What key factors should a lab consider when choosing the right workstation for their lab?

One of the first considerations is usually what size of workstation is appropriate for a laboratory’s requirements. In the case of Whitley Workstations, a range of capacities is available from 250 to 1400 petri dishes. However, it is not just size but also layout and useable space that makes a controlled atmosphere workstation truly efficient. At DWS, we believe that combining the working and incubation area (so that the whole interior is at incubation temperature) is the best way to maximize useful capacity.

Also high on the list of desirable features is rapid entry to and egress from the interior – both for operators and for samples or culture plates. A workstation with convenient rapid access encourages the transfer of plates to anaerobic conditions immediately after inoculation; this reduces the degree of oxygen exposure experienced by plates and this maximizes recovery of fastidious anaerobes. Better still, samples can be transferred into the workstation and plates inoculated within the anaerobic environment.

To ensure optimal results and aid compliance with laboratory quality standards such as ISO 17025, it is essential that anaerobic incubation conditions can be verified, for example by use of appropriate biological or chemical indicators. Modern anaerobic workstations can automate this process by continuously recording critical environmental parameters such as oxygen concentration, temperature and humidity in real time. In Whitley Workstations, the activity of the palladium catalyst responsible for removing trace levels of oxygen can also be regularly checked and recorded.

 

A135 Anaerobic Workstation

The Whitley A135 Anaerobic Workstation is the tallest, deepest and widest anaerobic workstation available

 

In the DWS range, what functions can be accessed remotely?
The latest Whitley workstations are controlled via a touch screen interface. This provides various “intelligent” functions and includes a network connection as standard. Any parameter that is controlled by the touchscreen can also be accessed remotely. These include graphical records of oxygen concentration, temperature, humidity, gas pressures and catalyst activity, each of which can be monitored remotely via a networked computing device.

DWS have recently introduced a new model – the Whitley A135 HEPA, what new features does this model offer?

The Whitley A135 HEPA is a tall, wide, deep anaerobic workstation fitted with the unique Whitley Internal HEPA Filtration System. It provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen. With a usable internal volume of 560 litres, a variety of equipment can be accommodated within the chamber. As well as providing ideal conditions for anaerobic studies, this workstation operates from two gas supplies for the most cost-effective running conditions.

• The built-in rapid airlock ensures samples can be transferred into the workstation atmosphere as soon as possible (takes only 60 seconds)

• Fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly.

 DWS filters are located inside the workstation, which prevents the filter becoming saturated with moisture and rendered ineffective.

 Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3. Proof of atmospheric cleanliness can be provided on request.

 Fitted with a removable front to facilitate thorough cleaning, easy replacement of the HEPA filter, and the transfer of bulk samples and larger pieces of equipment for use in the workstation.

 The internal atmosphere is circulated continuously – contributing to even temperature distribution and ensuring internal particulate will be in motion before capture by the filter.

 Fully integrated gas control avoids bulky add-on systems – reduces the need for additional bench space.

 A colour, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.

 Includes an automatic commissioning cycle to save you time and resources.

 Options include an integrated Anaerobic Conditions Monitor with data download facility.

DWS have many years’ experience with anaerobic workstations – what are the most common applications?
The main application for anaerobic workstations is routine clinical microbiology (hospital labs) but we have customers using them in a wide variety of work conducted by universities, including dental, veterinary, gut modelling research, preparation of faecal transplant material and the determination of the efficiency of cleaning agents. We even have a customer who uses an anaerobic workstation for soil research and a commercial enterprise in the oil industry researching bacteria that damage pipelines.


 Click here for more information on the Whitley A135 HEPA Workstation


1743_DonWhitley_AndrewPridmore

 

Dr Andrew Pridmore is Head of Microbiology at Don Whitley Scientific.

Dr Pridmore has many years’ experience of running the GLP-compliant, contract microbiology laboratories at DWS where Whitley Workstations are routinely used.


 

Whitley A95 Workstation for Jin Sung on final test

End of an Era for Don Whitley Scientific

Production comes to a close in Shipley

The last Whitley Workstation to be manufactured by Don Whitley Scientific in Shipley has now rolled off the production line. This particular order was an A95 Anaerobic Workstation ordered by Jin Sung Unitech, Republic of Korea.

JS Unitech LogoAccording to Junghoon Hong of Jin Sung, “We had no idea that in placing this order, we would be commissioning the last ever workstation to be built at Don Whitley Scientific in Shipley. We have been distributing Whitley Workstations for 19 years and are very proud to have ordered the last one from the Shipley production line.”

Paul Walton added, “I am extremely happy that we are moving to bigger and better premises that will provide the opportunity to improve our production processes. Victoria Works will provide considerably more warehousing capacity plus new offices, a bespoke production area, service centre, R&D mezzanine and improved staff facilities including a large, fully fitted, air-conditioned canteen. There will also be a new product showroom where customers can view demonstrations of the latest anaerobic and hypoxic workstation technology.

“An element of nostalgia is, however, creeping in as we draw close to the move date. We have been manufacturing in  Shipley for over 40 years and I have seen many changes in those four decades. We will, however, focus on the positives and move the business forward to face the challenges of the next 40 years!”

When Don Whitley Scientific began making workstations, it was purely for the UK market. We now have over 4,000 workstations in over 50 countries.

It might be the end of one era but another, exciting one begins at our new premises in Bingley.

Clostridium difficile studies can be done in a Whitley Workstation

An exciting opportunity to join our sales team

Anyone interested in an exciting opportunity to move into sales?

Don Whitley Scientific now has a vacancy for a Technical Sales Representative.

The Role

As a Technical Sales Representative you will promote an exciting range of laboratory equipment and service contracts. Brands include Whitley Anaerobic, Hypoxic and Microaerobic Workstations, Whitley Automated Spiral Plater plus products such as the automated specimen processor WASPLab, media/sample preparation equipment and other niche laboratory equipment.

This role will provide an opportunity for the successful candidate to further develop an established customer base and build a long-term, lucrative career in the UK as well as the possibility of overseas travel.

Candidates with no previous sales experience will be considered as training will be provided. A high level of support will also be available from senior colleagues.

 

Core responsibilities include:

  • Maximising sales potential and business growth by establishing relationships and collaborations with customers.
  • Working closely with marketing to ensure relevant promotions are delivered to customers and that feedback from customers is relayed back to inform future planning.

Essential Requirements:

  • Highly motivated and ambitious.
  • Hands-on approach and comfortable with daily travel.
  • Articulate, confident communicator able to engage with people at all levels, including technical managers and procurement/finance personnel.
  • Independent and decisive, using creative thought and sound logic to identify, prioritise and maximise opportunities.
  • Based in the north of England to serve a territory that extends into Scotland.

Our sales team comprises of successful people with and without a science background, so experience in a scientific discipline is not a prerequisite.

 

Remuneration

Package includes:

  • Basic salary dependent upon experience
  • Attractive bonus scheme
  • Company car

How to apply

Please email Steve Robertson, Sales Director on steve_robertson@dwscientific.co.uk attaching a current CV.

STRICTLY NO AGENCIES

removal van

Don Whitley Scientific to Move to Bingley

After 40 years of manufacturing in Shipley, Don Whitley Scientific Limited has announced that the company will move to new premises in Bingley.

 

 

 

 

Managing director, Paul Walton, explains:Paul Walton
“Our business has expanded considerably, particularly over the last 10 years, and despite having purchased numbers 16 and 18 Otley Road, we have still outgrown our current facilities. When Victoria Works came up for sale, providing 48,100 sq ft, we knew this would be the right move for us. The Bingley premises will provide us with the space to organise ourselves more efficiently and still have room to expand.”

 

 

 

The new premises, just off Bradford Road, is undergoing a major refit (by Eclipse Interiors) to make it suitable for the company’s needs. There will be considerably more warehousing capacity plus new offices, a bespoke production area, service centre, R&D mezzanine and improved staff facilities including a large, fully fitted, air-conditioned canteen. There will also be a new product showroom where customers can view demonstrations of the latest anaerobic and hypoxic workstation technology.

 

 

The office for sales, service and marketing

The office for sales, service and marketing

Warehouse area

Warehouse area

 

 

 

 

 

 

 

 

 

 

Product showroom

Product showroom

 

 

 

 

 

 

 

 

 

 

 

 

The move will take place from 13 to 17 June so the business will be operational from Bingley on Monday, 18 June.

 

Hypoxystation range video

New Whitley Hypoxystation Video Launched

Don Whitley Scientific are proud to present a new video that showcases our wide range of Whitley Hypoxic Workstations. The video also features comments from renowned researchers, explaining how a Whitley Hypoxystation benefits their research.

The video is another addition to a growing YouTube channel which features similar videos showcasing our wide product range and company values, including anaerobic and microaerobic workstations, customer testimonials, an introduction to Don Whitley Scientific and the level of service on offer for our customers.

 

 


Researchers featured in this video
Dr Karen Wright – Dr Karen Wright uses a Whitley H35 Hypoxystation in the cell culture laboratory at Lancaster University to expedite a component of her research, namely culturing human gut cells at low physiological levels of oxygen. – Testimonial in full

Dr Michael Cross – Dr Cross and his group use their H35 HEPA Hypoxystation to test cardiovascular drug toxicity in a cardiac spheroid model under physiologically relevant oxygen tensions and also periods of extreme hypoxia/ischemia. – Testimonial in full

 

Click here to explore the full range of Whitley Hypoxystations

 



Contact our sales team for more information on 01274 595728 or email sales@dwscientific.co.uk. For the full range of Whitley products, visit our website at www.dwscientific.co.uk


Clostridium difficile studies can be done in a Whitley Workstation

Hypoxic Snapshot Analysis in a 3D Engineered Tumour Scaffold

Researchers have long exposed 2 dimensional cells to varying degrees of hypoxia, often found in the tumour microenvironment. This can be great for showing overall hypoxic protein expression, but fails to account for cell to cell interactions, tissue gradients, and metabolic reprogramming. To more closely mimic in vivo conditions, a 3D culture environment is necessary. While 3D culture is nothing new, Rodenhizer et al in their paper “A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients” found a novel way to culture in a 3D environment while maintaining the ability to perform analyses that only function in 2D. These ingenious authors did all of this in their Hypoxystation by Don Whitley Scientific. They were able to analyse and map cellular metabolism and spatially identify known and novel metabolic response to hypoxia.

The team took carcinoma cells and seeded them onto a novel rolled scaffold system the authors termed “TRACER”. They exposed the scaffold to varying degrees of hypoxia, down to as low as 0.2% O2. After incubation they unrolled the 3D scaffold and started analysis to create a metabolic snapshot of the tumour. They found the 3D scaffold tumours displayed different concentrations of metabolites as compared to 2D tumour cells, implying cell to cell interactions plays a role in tumour metabolism, depending on the depth of the cell in the scaffold. The future of cancer research will be shaped by this innovative technique and oxygen control plays an important role with use of the Hypoxystation.

single layer TRACER

Image from Nature Materials Supplementary Information, “A Three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism phenotype in hypoxic gradients”

 

The Don Whitley Scientific Hypoxystation is ideal for many applications, as it can control oxygen down to 0.1% while providing a temperature and humidity controlled environment with ample working space. ISO class 3 clean room HEPA filtration is also available for long term cell culture applications.

blackburn wasp

WASP and A55 Anaerobic Workstation installed at Royal Blackburn Hospital

Royal Blackburn Hospital recently made some key improvements to their laboratory. The installation of both the Walk Away Specimen Processor (WASP) and the Whitley A55 Anaerobic Workstation will increase efficiency and productivity in their routine microbiology and MRSA work.

WASP is an automated specimen processing system that can be connected to a laboratory’s LIS. Previously Don Whitley Scientific has installed WASP™ systems under the guidance of Copan, the system’s manufacturer. This installation and connection to the LIS was carried out solely by Don Whitley Scientific, providing a solution in automation for this busy lab.

The department at Royal Blackburn Hospital will use the system to process MRSA samples alongside other routine microbiology applications. WASP™ and WASPLab™ represent a true modular, scalable, long-term solution for the efficient automation of specimen processing in bacteriology laboratories, allowing for: plate inoculation (streaking), gram slide preparation, enrichment broth inoculation, robotic incubation and management, Kirby-Bauer application, MALDI-TOF target plate management and plate image recording and analysis.

A Whitley A55 Workstation was also installed at the hospital. The A55  is one of the largest workstations in the DWS anaerobic range. One of the key benefits of this particular workstation is a large incubation area that accommodates up to 1,400 x 90mm Petri dishes, providing a huge working capacity under strict anaerobic conditions. The workstation also comes equipped with two airlocks. Each airlock allows 40 x 90mm Petri dishes to be introduced and withdrawn at either end of the workstation offering maximum flexibility and improving the workflow in a laboratory. Both airlocks can be operated at the same time. The A55 replaces older Whitley Workstations used by the lab and should improve efficiency and increase the lab’s potential. The WASP™ and Whitley A55 will both provide substantial benefit to work being undertaken at Royal Blackburn Hospital.

Don Whitley Scientific has been manufacturing anaerobic workstations for decades whilst providing excellent service and support for our customers. For the past few years we have been installing WASP™ and WASPLab™  units in locations around the UK.

 


If you would like more information on WASPLab or any other product in the Don Whitley Scientific product range, please call +44 (0) 1274 595728 or email sales@dwscientific.co.uk


 

 

WASP Touch

WASP Touch and ProtoCOL3 play key part in Australian Lab

A research and development lab at the New South Wales Department of Primary Industries (Sydney, Australia) recently purchased a WASP Touch Spiral Plater and ProtoCOL3 colony counter. The lab is carrying out research on food safety of fresh horticultural produce and nuts. The head of the research program, Dr SP Singh, spoke to Don Whitley Scientific to explain how these two new products, provided by DWS, are key to his team’s working processes.

Dr Singh and his colleagues are exploring fresh options when it comes to food safety: “We are developing new ways of sanitising the produce by killing potential foodborne bacterial pathogens”. The produce his lab are sanitising is often used for export, meaning this is a vital process to ensure safety for the consumer. Dr Singh explains that “we have to deal with hundreds of samples everyday” meaning “plating and enumeration is a core lab activity”.

The lab’s project was recently featured on NBN News – http://www.nbnnews.com.au/2017/11/10/world-first-food-sanitiser-at-ourimbah/. The project is a world’s first and to sanitise food without the use of chemicals could be a revolutionary step forward in food safety.

Speaking about his decision to invest in the DWS products, Dr Singh said “we were looking to improve lab productivity and resource use efficiency by switching to spiral plating and automatic counting”.

The Whitley WASP Touch is the latest spiral plater from Don Whitley Scientific (who have been manufacturing spiral platers since their inception in the 1970s). WASP Touch provides real cost savings and process improvements, as well as eliminating the time-consuming process of serial dilution.

The ProtoCOL3 (from Synbiosis) facilitates automated colony counting and zone reading using a hi-specification digital camera and LED lighting. The system comes equipped with a touchscreen PC for analysing samples. Another positive that we provided to Dr Singh was the fact Don Whitley Scientific was able to provide both of these products, making the acquisition of vital equipment more straight forward: “Don Whitley Scientific offered this integrated solution of automatic plating and counting”.

To summarise, the WASP Touch and ProtoCOL3 should provide improvements to the efficiency and working methods of Dr SP Singh’s revolutionary lab.

 

Dr S.P. Singh's Lab

Dr SP Singh’s Lab

 

 

The WASP Touch and ProtoCOL3 should provide improvements to the efficiency and working methods of DR SP Singh’s revolutionary lab. To find out more about the Don Whitley Scientific product range in the UK click here, call us on +44 (0) 1274 595728 or email us at  sales@dwscientific.co.uk

For Don Whitley Scientific Australia click here call us on (02) 4339 1029 or email us at  sales@dwscientific.com.au

 

 

 

 

Whitley Hypoxystation

Hypoxia and the Hallmarks of Cancer: Metabolic Reprogramming

Hanahan and Weinberg’s seminal papers on the Hallmarks of Cancer describe how cancer cells accommodate the frenzied growth characteristic of tumours. Low oxygen is eminently characteristic of tumours, and in this hypoxic environment, metabolism is reprogrammed to satisfy energetic and synthetic needs of the cells.

 

Our series on Hypoxia and the Hallmarks of Cancer has showcased research on how hypoxia in the tumour microenvironment affects 8 of the Hallmarks, and in the fifth and final chapter, we look more closely at how researchers are using the Hypoxystation to delineate the Hallmark Metabolic Reprogramming.

The Hypoxystation creates authentic cell culture conditions with regard to oxygen, CO2, temperature, and humidity.  Glove-less access to culture and manipulate cells under physiological atmosphere, in a HEPA-clean environment, allows cancer researchers to re-create the hypoxic tumour microenvironment. Hypoxystation user Dr Ali Tavassolli states that “We have only ever used the H35. I like the ease with which we can regulate and change the oxygen concentration”. And our user Dr. Brad Wouters at the Princess Margaret Cancer Centre in Toronto, who recently purchased his fourth Hypoxystation, says, “The continuous hypoxia we achieve in the workstation is a prerequisite for studies with hypoxia-activated drugs used in cancer therapy strategies.”

Hallmarks of Cancer

Metabolic Reprogramming

Changes in energy metabolism feature prominently in aggressive malignancy, and tumour hypoxia and the responding signalling pathways, featuring many HIF target genes, clearly interface with reprogrammed tumour metabolism. Reprogramming of conventional metabolic pathways serves to satisfy burgeoning energetic and anabolic needs of the tumour cells; many cancer cells may preferentially utilise glycolysis over oxidative phosphorylation, uncoupling mitochondrial metabolism from oxygen availability. Hypoxia-induced HIF’s attenuate mitochondrial function through diverse mechanisms, including down-regulation of enzymes in the electron transport chain and suppression of biogenesis of mitochondria. Signalling pathways involving HIF’s and many products of oncogenes and tumour suppressor genes interact to balance the energy needs of dividing cells with the requirement for bio-synthetic intermediates. Activation of lipid biosynthesis and other pathways with biosynthetic significance, such as the pentose phosphate pathway, is another metabolic consequence of hypoxia and HIF up-regulation. Reactive oxygen species ROS produced by the mitochondria stabilise HIF-1, influence redox homeostasis, and provide protective antioxidants to the cancer cells.

reprogrammingSliceLITERATURE: