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Archive for April, 2015

H35 Hypoxystation: Research Applications

Our US distributor, HypOxygen, recently conducted several interviews with scientists using the Whitley H35 Hypoxystation, in order to find out exactly what kind of work they are doing, and how our workstations are helping in their research:

Professor Biplab Dasgupta from the UC Department of Paediatrics at Cincinnati Children’s Hospital spoke about his interest in the development of the central nervous system, particularly the brain in the light of cellular metabolism, and his work on neuronal tumours. You can read the full interview here.

Whitley H35 Hypoxystation

Whitley H35 Hypoxystation

 

Dr Houda Benlhabib from the Department of Biochemistry at UT Southwestern discussed her research into the role of genetic and epigenetic mechanisms in the regulation of foetal lung development as well as in reproductive and perinatal biology. Click here to find out more.

Professor Margaret Petroff from the Department of Anatomy and Cell Biology at the University of Kansas Medical Center, is working on foetal antigens and maternal recognition of the foetus during pregnancy, autoimmune diseases and their impact on fertility, and the relationship between pregnancy and cancer.

Dr Caitlin Linscheid was interviewed on Professor Petroff’s behalf, you can read what she said here.

Distributor of the Year Award 2014

Top Distributor Award 2014

Congratulations to the team at Microbiology International (USA), who sold more equipment than any of our other distributors last financial year, and have been awarded Top Distributor of the Year 2014.

 

Microbiol. Int

Don Whitley Scientific has enjoyed a mutually-rewarding working relationship and friendship with Microbiology International for over two decades, and we look forward to many more years of successful collaboration.

 

 

H135: Taller, Deeper, Wider.

Don Whitley Scientific is proud to present the new Whitley H135 Hypoxystation; the tallest, deepest, widest workstation in our range.

This new hypoxic chamber has a usable internal volume of almost 600 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc. (Be sure to discuss your requirements with us at the earliest opportunity). The generous internal height also facilitates easy pipetting. All cell manipulations can be performed without removing them from your required hypoxic conditions.

With a volume twice that of a H35 Hypoxystation, the H135 also comes fitted with a large removable front, available with either two or three ports, plus the same great features and benefits as other Whitley Workstations, including:

  • Rapid 12 litre airlock

    H135 Hypoxystation

    H135 Hypoxystation

  • Three gas operation
  • The Whitley Internal HEPA Filtration System with Enhanced Biological Containment
  • The option of being connected to a Whitley i2 Instrument Workstation
  • 7” full colour touch screen that is Ethernet-enabled for remote access.

Internal dimensions (w x d x h) are 1100mm/43.3” x 750mm/29.5” x 710mm/28”.

External dimensions (w x d x h) are 1452mm/57.25″ x 1056mm/41.5″ x 993mm/39″.

Representatives from Don Whitley Scientific will be attending the Keystone Symposia in Dublin, 12-17 May, where you can be one of the first to see this revolutionary product.

Watch the introductory video here.

 

 

New Poster from Liverpool University

Researchers from the University of Liverpool (Department of Infection Biology, Institute of Infection & Global Health and School of Veterinary Science), have produced a poster entitled “Understanding the aetiology and infection reservoirs of digital dermatitis in beef cattle and sheep”.

As part of their work, they cultured treponemes in a Whitley A35 Anaerobic Workstation.

Whitley A35 Anaerobic Workstation

Whitley A35 Anaerobic Workstation

Abstract:

Digital dermatitis (DD) is an infectious hoof disease causing severe lameness in ruminants worldwide. The primary cause has been identified as the spirochaetal bacteria, treponemes. A comprehensive bacterial molecular survey of sheep contagious ovine digital dermatitis (CODD) lesions has not been done to determine if there is a shared etiopathogenesis between BDD and CODD. Also, there have been only anecdotal reports of BDD lesions occurring in beef cattle, with no definitive published data on the disease.  Additionally, little is known about the transmission of Treponema bacteria. It may be possible that DD treponemes are carried in both cattle and sheep gastrointestinal (GI) tracts and then shed in faeces and spread. Another route of transmission may be via farm equipment.

This study investigated ruminant GI tissues and farm equipment for the presence of DD treponemes. Additionally, sheep and beef DD lesions were analysed for the presence of DD treponemes by PCR and culture techniques.

The full poster can be viewed  here.

HypOxygen at AACR Meeting

Our American distributors, HypOxygen, are exhibiting Whitley Workstations at this year’s AACR meeting in Philadelphia. Go over to their stand to see the i2 Instrument Workstation (specifically designed to house a Seahorse XF Analyzer in physiologically relevant conditions), H35 Hypoxystation and also to learn about our range of HEPA workstations.

EK, BKF at AACR2Pictured here is Evan Kitsell, Design Director at Don Whitley Scientific Ltd, and Dr Burga Kalz Fuller, Product Manager at HypOxygen. They will be available to demonstrate the workstations and answer any questions you may have.

As the AACR‘s website describes: this year’s meeting will “highlight the latest, most exciting discoveries in every area of cancer research and will provide a unique opportunity for investigators from all over the world to meet, interact, and share their insights. This year’s meeting theme – Bringing Cancer Discoveries to Patients – underscores the vital and inextricable link between discovery and treatment, and it reinforces the fact that research underpins all the progress we are making in the field toward cancer cures.”

 

 

 

Cell Press Webinar

Cell Press Webinar: Hypoxic Microenvironments

The hypoxic microenvironment triggers many of the events underlying cancer progression and stem cell maintenance and/or differentiation. In maintaining oxygen homeostasis, oxygen levels are sensed by mitochondria, communicated by reactive oxygen species (ROS), and conveyed by hypoxia-inducible factors HIF’s. Hypoxia signalling pathways have far-reaching consequences for cellular and tissue processes from metabolism through angiogenesis to metastasis.

Dr. Hannele Ruohola-Baker (University of Washington) and Dr. Randall Johnson (University of Cambridge) will be speaking at the upcoming Cell Press webinar on “Hypoxic Microenvironments”, which Don Whitley Scientific and HypOxygen are proud to sponsor. Dr. Ruohola-Baker’s lab investigates metabolic determinants of and miRNA function in stem cells, and Dr. Johnson examines the effects of hypoxia in cancer and inflammation.
Read more

AACR logo

AACR Poster Session

A team of scientists from the Brain Sciences Division, Hammersmith Campus, Imperial College London, have successfully submitted an abstract to the AACR, and their poster will be presented at the upcoming meeting in Philadelphia, April 18-22.

Some of the research for this poster, entitled “Arginine deprivation using ADI-PEG20 leads to regression of an ASS1-ve intracranial GBM tumor in mice and potentiates gamma irradiation of ASS1+ve GBM in vitro”, was conducted in a Whitley H45 HEPA Hypoxystation.

The poster will be on display at the Late-Breaking Research: Experimental and Molecular Therapeutics session, Sunday April 19, 2015 between 1 PM and 5 PM.

Read more

i2 and H35 HEPA at ICR London

Whitley i2 Instrument Workstation used in deciphering the metabolic properties of Breast Cancer

The Institute of Cancer Research, London, is Europe’s largest cancer centre and one of the world’s most influential research institutes, with an outstanding record of achievement dating back more than 100 years.

Staff at the ICR with their new Whitley Workstations

ICR staff with their i2 and H35 HEPA Workstations

Cancer cells display unique alterations in their signalling and metabolic circuitry in order to fuel their growth and proliferation and/or adapt to conditions of oxygen and nutrient deprivation. The Signalling and Cancer Metabolism Team, led by Dr George Poulogiannis uses high-throughput technologies including mass spectrometry-based metabolomics, and utilizes the Seahorse XF Analyzer to measure the basal oxygen consumption and glycolysis rates in order to reveal the metabolic dependencies of breast cancer-driven alterations. We are using a Whitley i2 Instrument Workstation to study the signalling and metabolic pathways that may preserve breast cancer cell viability under hypoxia. In addition to this, we have a general interest in identifying what signalling and metabolic pathways are associated with the acquisition of microsatellite (MSI) and chromosomal instability (CIN) under oxygen deprivation conditions. Finally, we are studying the role of hypoxia in cell invasion and metastasis, oncogene-induced senescence and resistance to current treatment options.

George Poulogiannis
Team Leader
Division of Cancer Biology
Institute of Cancer Research, London

TShirt for Survey

Free T-Shirt: Hypoxia Research

 

Do you culture cells in hypoxic conditions? For a limited time only, you can earn a free t-shirt by taking a few minutes to answer our questions and help us to better fulfil your cell culture needs.

We want to know what your research focus is, how you conduct your cell culture and how you think oxygen content might influence aspects of your cell culture.

Please click here to complete a very short online form.