DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Biomedical Scientist_Clinical Microbiology of Anaerobes Course

Campylobactor Research with Microaerobic Workstations

The following words were provided by Microbiology International 

Cultivation of microaerophilic organisms from environmental niches such as Campylobacter species and Helicobacter pylori requires precise calibration of oxygen levels and other growth parameters. Microbiology International, North American distributor for Don Whitley Scientific, have been installing anaerobic and microaerobic incubation chambers for more than 20 years, and would like to introduce some of the research being carried out around the world. Click here to view our Microaerobic Workstations.

The complex interactions of microbial communities populating the human gastrointestinal tract with their host and with invading pathogens are paramount to safeguarding not only a healthy gut but also our general health. Microaerophilic bacterial species, such as Campylobacter jejuni, can cause gastro-enteric infections due to their ability to survive and grow in lower oxygen environments, which they encounter in the human gastro-intestinal tract. At the Ottawa Institute of Systems Biology, Dr. Alain Stintzi researches topics as diverse as iron homeostasis and oxidative stress and the competitive advantage of metabolizing L-Fucose in Campylobacter jejuni. Using a Don Whitley Scientific microaerobic workstation, Dr. Stinzi states that “One objective of our research is to understand how enteric pathogens such as Campylobacter jejuni acquire essential nutrients, adapt to the harsh conditions of the intestine and interact with the host’s microbiota to cause disease.”

At Ohio State University, Dr. Jeffrey Lejeune’s research is focused on prevention of diseases caused by food-borne pathogens in plants and animals, including C. jejuniE. coli O157, and Clostridium difficile. His work on antibiotic resistance in Campylobacter and other enteric pathogens has prompted the UN’s Food and Agriculture Organisation to recruit him to provide technical support and guidance in an international group working on antibiotic resistance. Don Whitley Scientific anaerobic and microaerobic workstations , sold in the US by Microbiology International, provide precise gas control for a sustainable low oxygen environment. Up to four gasses can be combined to create the ideal atmosphere for fastidious microorganisms.

In the UK, Dr. Andrew Grant at the University of Cambridge is investigating the Campylobacter jejuni secretome and diarrhoeal disease in a gnotobiotic piglet model, using the MACS VA500 and more recently, the M95 Microaerobic Workstation. His work to elucidate host-pathogen interaction and virulence strategies will yield new options for therapy and vaccination. Don Whitley Scientific will be there for him, and our other researchers, every step of the way.

 

From: Stahl et al. (2011) “L-fucose utilization provides Campylobacter jejuni with a competitive advantage“ Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7194-9

From: Stahl et al. (2011) “L-fucose utilization provides Campylobacter jejuni with a competitive advantage“ Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7194-9

From: Tang et al. (2017) “Rising fluoroquinolone resistance in Campylobacter isolated from feedlot cattle in the United States” Scientific Reports 7: 494

From: Tang et al. (2017) “Rising fluoroquinolone resistance in Campylobacter isolated from feedlot cattle in the United States” Scientific Reports 7: 494

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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IBMS Success for Don Whitley Scientific

Don Whitley Scientific once again exhibited at the Institute of Biomedical Science Congress. This bi-annual three day event is held at the International Convention Centre in Birmingham.

The event combines business, networking and social exchange and enables delegates to view new equipment and products, extend general awareness of techniques and technologies and make contact with representatives of exhibiting companies.

This year the Don Whitley Scientific exhibition area was purpose-built to accommodate the cutting edge technology on show. This included the Walkaway Specimen Processor (WASPLab), the Whitley A35 Anaerobic Workstation, and ChromaZona from Synbiosis, the new automated microbial identification AST analysis system. Members of the DWS sales force were on hand to deal with any customer queries.

The stand also featured an area where delegates could interact with our virtual lab, an interactive presentation where our product range could be explored in detail.

As is the custom with at IBMS, we also held a competition. This year we celebrated 40 years of serving the microbiology industry, with Love2Shop vouchers on offer for the lucky winners. Visitors to the stand needed to look for clues in a video presentation, which would give them the answers to a range of questions.  And each and every visitor to our stand had the opportunity to further indulge in a corporate cupcake.

 

 

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Whitley Hypoxystation

World Heart Day: Hypoxia in Cardiovascular Disease Research

Cardiovascular disease, including heart disease and stroke, is responsible for approximately 1 in 3 deaths in the US, according to the American Heart Association. World Heart Day on 29 September serves as a platform to educate people on how to take control of their heart health.

Don Whitley Scientific and our US/Canadian distributor HypOxygen would like to take this opportunity to highlight cardiovascular research being carried out around the world – and to say “thank you for being committed to our health.”

Adverse cardiac remodeling after infarction exacerbates myocardial ischemia and increases the likelihood of heart failure. Revuelta-Lopez et al. in Spain present new data showing that in the hypoxic areas of the infarct zone, expression of low-density lipoprotein receptor-related protein 1 (LRP1) is linked to activation of Matrix metalloproteinase (MMP) through Pyk2 phosphorylation, and propose that LRP1 modulation may be a very effective pharmacological target in heart disease. Their H35 Hypoxystation with its controlled low oxygen environment creates physiologically more relevant parameters for cell culture, mimicking ischemia/reperfusion events.

Hypothesizing that Tumor necrosis factor-Related Apoptosis-Inducing Ligand plays a role in ischemic injury during acute myocardial infarction, Jiang et al. have found evidence for a novel immune regulatory mechanism involving TRAIL, ER stress and NF-κB signaling pathways. Culturing their cells in the Hypoxystation H35 at 0.3% oxygen allowed the lab to simulate the ischemia/reperfusion processes that cause cardiomyocyte loss and increase mortality in Coronary Heart Disease.

Hypoxia in the embryonic environment supports maintenance of a primitive glycosaminoglycan-rich heart valve matrix, the specific composition of which determines proper function, and as hypoxia decreases after birth, the extracellular matrix matures. Amofa et al. at Cincinnati’s Children’s Medical Center, using the H35 Hypoxystation, provide new data that exposure of adult heart tissue to hypoxia induces hyaluronan remodeling, GAG accumulation, and degeneration of the extracellular matrix in the heart valve, effects that are implicated in Myxomatous mitral valve disease.

Dr. Michael Cross, Molecular and Clinical Pharmacology Department, University of Liverpool, says of his work with cardiac spheroids : “The H35 allows us to generate oxygen levels that reflect the in vivo physiology these cells would be exposed to. We chose the Hypoxystation with its oxygen profiling feature, which allows us to recreate cycles of ischemia, where oxygen levels typically sink to 1-3%”.

Revuelta-Lopez et al 2017

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Image from: Revuelta-Lopez et al. “Relationship among LRP1 expression, Pyk2 phosphorylation and MMP-9 activation in left ventricular remodelling after myocardial infarction” J Cell Mol Med. 2017 Sep;21(9):1915-1928

 

Amofa et al 2017

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Hypoxia increases GAGs, Sox9 nuclear localization and Hyal2 expression in cAVOCs.

Image from: Amofa et al. (2017) “Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves” Am J Physiol Heart Circ Physiol. 2017 Aug 25:ajpheart.00209.2017

 

 

 

DWS Exhibit at IBMS 2017

The Don Whitley Scientific team are at IBMS this week, pictured here at our exhibition stand, demonstrating the A35 Anaerobic Workstation, WASP Flo for WASPLab™, and Chromazona. To learn more about these and our extensive range of other products, come along to stand 407 and talk to one of our Product Specialists.

You could also take part in our competition – we are celebrating 40 years in business and would like you to help us commemorate this milestone with a fun quiz and the chance to win £40 worth of Love2Shop vouchers.

 

 

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Celebrate with DWS and Win! – IBMS 2017

Visit the Don Whitley Scientific stand at this year’s IBMS Congress and enter our fun quiz to win £40 worth of shopping vouchers

If you are attending this year’s Biomedical Science Congress then come and take part in our competition – we’re celebrating being in business for 40 years. 40 years serving science is quite an achievement and we would like you to help us commemorate this corporate milestone with a fun quiz!

Ask at stand 407 for an entry form.

The entry form features 6 simple questions about Don Whitley Scientific’s history: from humble origins to the state-of-the-art products and services we offer today.

The first correct entry drawn each day (25th, 26th + 27th September) wins £40 worth of Love2Shop vouchers.

We will contact all winners by phone or email so you can come and collect your prize or arrange to have it delivered to you.

You will also receive a Don Whitley Scientific pen just for entering the quiz.

GOOD LUCK

Don Whitley Scientific will be exhibiting at IBMS next week from 24th – 27th September. Visit our stand to see what products we have on show and join us in our 40 year’s serving science celebration

Clostridium difficile studies can be done in a Whitley Workstation

Hallmarks of Cancer: Sustaining Growth and Resisting Cell Death

In part four of our mini-series describing “Hypoxia and the Hallmarks of Cancer”, we look more closely at how researchers are using the Hypoxystation to delineate the Hallmarks Sustaining Growth and Resisting Cell Death.

 

 

 

 

 

 

Resisting Cell Death

The ability of cells to resist cell death under hypoxic conditions is central to the progression of cancer and the acquisition of resistance to chemotherapy so frequently encountered in tumors. Hypoxia in the tumor microenvironment exerts selective pressure favoring cells that have lost the functionality of apoptosis genes and can expand uncontrollably.  Hypoxia also contributes to survival by inducing autophagy, in a pathway involving HIF-1, beclin, BNIP3 and BNIP3L, in which cellular autophagy acts to recycle cellular organelles, satisfy metabolic demand and improve hypoxic tolerance.  HIF-1 mediates cell-cycle retardation and arrest, causing hypoxic tumor cells to become resistant to radiotherapies. NF-κB, through its effects on myriad transcription factors, for example through inhibition of cell death signalling, is activated by hypoxia and reactive oxygen species, and also promotes cell survival.

Sustaining Growth

Cancer is essentially based on the cells’ inability to “stop” when suppressors signal an end to growth, and the compunction to “go” despite a lack of bonafide growth signals. Hypoxia in the context of cancer, in precipitating genomic instability and mutation, results in numerous inactive tumor suppressor genes and activated growth factor genes, such that the combination of constitutive proliferative signaling and mutated cancer genes leads to sustained growth. HIF and NF-κB regulated pathways involving Notch, mTOR, WNT11, CAIX, and IGF-1, among many others, contribute to sustained growth in cancer as regulation of proliferation derails. Induced by hypoxia-regulated proteins, anabolic pathways for nucleotide and lipid synthesis are ramped up and enable the rapid proliferation typical of cancer.

SustainingGrowthSliceLITERATURE:

 

 

 

 

Celebrating 40 Years Graphic

Invitation to Don Whitley Scientific’s birthday celebrations

Your Invitation

Don Whitley Scientific has now been in business for 40 years and will be celebrating this milestone achievement during the IBMS Congress 2017.

On Monday, 25th September from 17:00 to 18:00 we will be hosting a drinks reception on Stand 407, Hall 3 and would be very pleased if you could join us.

If you are able to attend, please RSVP to sales@dwscientific.co.uk before 18th September (to help us with catering requirements).

 

The IBMS Congress is held at the International Convention Centre in Birmingham from 25th to 27th September 2017.

 

We look forward to seeing you.

 

A35_Front

Rest Assured That Your Anaerobic Workstation is at Zero Oxygen

The following was written by Microbiology International, Don Whitley Scientific’s supplier of the Anaerobic Workstation in the US

Don Whitley Scientific have turned the maintenance and monitoring of stringent anaerobes in their anaerobic workstations into an art. The unique combination of anaerobic conditions monitoring (ACM) and catalyst monitoring available on the  A35, A45, A55, A85 and A95 workstations guarantees that strict anaerobes really will be well within their comfort zone. Microbiology International is the North American distributor for these anaerobic workstations.

The ACM comprises of an oxygen sensor placed inside the workstation and software to process real-time data on oxygen levels in the chamber. Based on knowledge of bacterial oxygen tolerance, results are shown on the touchscreen interface as a colour-coded indicator of O2 concentration. Green, yellow, or red status bars clearly indicate to the user whether oxygen levels are acceptable and provide information on the correct functioning of the workstation. This fully automated system replaces resazurin strips, which can dry out and are subject to interpretation based on the pink-to-white colour change.

The palladium catalyst used to remove traces of oxygen in the Don Whitley Scientific anaerobic workstations is protected through the addition of Anotox, which removes volatile organic compounds and hydrogen sulfide from the chamber atmosphere. Proper functioning of this vital system is monitored by the patented Catalyst Monitoring System, which tests the function of the catalyst overnight, and also confirms that the necessary hydrogen is present.

Together, the anaerobic conditions and catalyst monitoring systems provide unambiguous proof that anaerobiosis is being maintained in the workstations. The art of “zero oxygen” is critically important to cultivating anaerobic microorganisms that, due to their fastidious nature, are often classified as “unculturable“. Taking advantage of the closed workstation format to establish a strictly controlled anaerobic atmosphere, researchers are using Whitley Workstations to examine diverse topics such as the degradation of complex glycans by human gut microbiota;  the rise in C. difficile infections through lawn contamination; and the anaerobic etiology of brain abscesses.

The A35 Anaerobic Workstation will be on the Don Whitley Scientific stand at this years IBMS Congress in Birmingham


 

 

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Products to be shown on DWS stand at IBMS 2017

It has today been announced that the Don Whitley Scientific stand at this year’s IBMS Congress and Exhibition (No 407) will feature the following:

NEW: WASPFlo for WASPLab™, the fully automated, barcode-driven, conveyor-connected system with remote plate image analysis and automatic incubation. WASPFlo is an automatic bulk sample sorter that will introduce dramatic efficiencies and revolutionise workflow. Also ask for information on the new PhenoMATRIX™, a sophisticated suite of algorithms that uses AI to automatically count and recognise organisms, giving microbiology labs the ability to read, interpret and segregate bacterial cultures with the click of a button.

Whitley A35 Workstation, the industry leading anaerobic cabinet that provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen. This workstation provides the ability to manipulate samples in a sustainable environment where parameters can be altered to create the required conditions. Come along and learn about all the options and accessories that can tailor this, and other workstations in the range, to your specific applicational requirements.

Have you experienced ChromaZona yet? This automated microbial identification and antibiotic susceptibility testing (AST) analysis system provides faster results in busy clinical laboratories. Use ChromaZona to generate objective, repeatable, traceable data in double-quick time and to EUCAST standards. The sensitive CCD camera, unique lighting system and powerful Chromogenic ID software provide identification of microbial species in seconds. The system automatically reads different plate types up to 150mm diameter and generates true-to-life, full colour images. ChromaZona can be connected to your LIMS to store data and generate reports in Excel…  say goodbye to transcription errors!

 


Visit Stand 407 or call to book your personal demonstration – 01274 595728.


Clinical microbiology at the IBMS

Announcement of DWS attendance at IBMS

Don Whitley Scientific will be exhibiting at the 2017 IBMS Congress in Birmingham in September. We’ll be on stand number 407, Hall 3.

The event is being held from 25 to 27 September 2017 at the International Convention Centre. This is an invitation to our customers, potential customers, distributors and suppliers to visit us over this 3-day conference and exhibition.

Come along and see why WASPLab has developed into a market leading, fully automated, barcode-driven, conveyor-connected system with remote plate image analysis and automatic incubation. New WASPFlow is an automatic bulk sample sorter that will introduce dramatic efficiencies and revolutionise workflow.

On show will be the latest innovations in anaerobic workstation technology. Our patented Instant Access Ports are now widely accepted as the industry standard, as is the Catalyst Monitoring System that provides an early indication of the deterioration of anaerobic conditions.

ChromaZona Screen You will also be able to experience ChromaZona, an automated microbial identification and antibiotic susceptibility testing (AST) analysis system that provides faster results in busy clinical laboratories.

Not yet registered?

For more information about the IBMS Congress and Exhibition or to register to attend (it’s free to attend the exhibition), visit the Congress website.