DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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The Leukemic Stem Cell Niche: Adaptation to “Hypoxia” Versus Oncogene Addiction

Hematopoietic stem cells (HSC) are responsible for constantly maintaining and replenishing the supply of new blood and immune cells. They give rise to both lymphoid and myeloid progenitor cells, which then proceed to differentiate down their respective paths to form various specialized cells such as erythrocytes, macrophages, B and T cells, to name a few. Within the body, HSCs are found to reside in extremely low oxygen environments called stem cell niches (SCN). Like all other regulated cell cycles, HSCs can lead to cancers such as leukemia and lymphoma if cell division becomes uncontrolled.

MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

Figure 2: Suppression of oncogenic proteins driving non-CML blood neoplasias in the course of cell “adaptation to hypoxia.” MEL (a), Kasumi-1 (b), or NB4 (c) cells were incubated in atmosphere at 0.1% O2 and lysed at the indicated times, and total cell lysates were subjected to immunoblotting with the indicated antibodies. GAPDH, H4, or ARD1 were detected to verify loading equalization. Migration of molecular weight markers is indicated on the left (kDa). For each cell population, one out of three independent experiments with similar outcome is shown.

In this paper, Cheloni et al. primarily focused on chronic myeloid leukemia (CML).

The authors hypothesized that suppression of the BCR-Abl oncogene is likely a key positive regulator of LSC survival within “hypoxic” SCNs. To analyse the various mechanisms and responses that CML cells demonstrate within SCNs, the authors studied correlations between varied oxygen and glucose concentrations with the amount of BCR-Abl produced. All tests were performed using two human CML cell lines, K562 and KCL22. Testing conditions were precisely controlled to mimic the SCN environment as close as possible. Using a Hypoxystation supplied by Don Whitely Scientific, a water-saturated atmosphere comprising of 0.1% O2, 94.9% N2 and 5% CO2 was generated and maintained.

The research conducted and documented by Cheloni et al. has provided great insight into several key regulatory mechanisms associated with leukemic stem cells as well as an explanation for their notorious reputation for having high relapse rates. Additionally, they established that the triggering of oncogene suppression associated with CML is due to severe energy restriction rather than simply the “adaptation to hypoxia.”

The Don Whitley Scientific Hypoxystation provides the user incredible flexibility as it can control oxygen down to 0.1% while providing a temperature and humidity controlled environment with ample working space. The extreme precision provided by the Hypoxystation was critical to the collection of accurate and reliable data when recreating the demanding environment that comprises SCNs. The atmosphere is constantly monitored and adjusted by the real-time feedback system to ensure accuracy and ISO class 3 clean room HEPA filtration is also available for long term cell culture applications.

Choose your atmosphere with the Hypoxystation hypoxia chamber. Accurately control O2, CO2, Temperature and Humidity.   

Hypoxystation is the only hypoxic chamber purpose built for physiological cell culture research. Specifically designed to create normoxic, hypoxic and anoxic conditions within a controlled and sustained workstation environment, this hypoxic incubator is ideal for research requiring the ability to accurately control O2, CO2, temperature and humidity. The Whitley Internal HEPA Filtration System provides a particle-free internal environment that exceeds ISO 14644 class 3 clean atmosphere.  With such accurate control and the ability to manipulate cells in situ without altering the incubation environment, research into cell biology can be performed over a comprehensive range of oxygen tensions with precision. Don Whitley Scientific offer the following range of Hypoxystations:

                                                                                   

Hypoxystation H35

Hypoxystation H45

Hypoxystation H85

Hypoxystation H135

i2 Instrument Workstation

Application possibilities for Hypoxystation are endless; it is being used for research into tumour microenvironment, hypoxia pathways and HIF signalling, in vitro modelling of in situ environments, cancer cell metastasis, angiogenesis, and many other fields where cells may benefit from a more physiological atmosphere.

 

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Poldip2 governs cellular metabolism under hypoxic conditions

Until recently, it was understood that the addition of the prosthetic group lipoate is essential to the activity of critical mitochondrial catabolic enzymes; however, its regulation was unknown. In their paper “Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer“, Paredes et al. prove that the Poldip2-controled lipoylation of αKDH and PDH is regulated under hypoxia and in cancer cells, most notably in triple negative breast cancer cells (TNBC).

Using a Don Whitley Scientific  H35 Hypoxystation to establish accurate hypoxic environments, the authors were able to manipulate Poldip2 expression, allowing them to make substantial contributions to the understanding of Poldip2’s essential role in metabolic function during both normoxic and hypoxic conditions. Additionally, they postulate that Poldip2 deficiency may specifically impact the fate and availability of Ac-CoA due to impaired TCA cycle function, potentially providing cancer cells with an advantage for cell proliferation or hypertrophy. Further research will require investigating the interplay between Poldip2 and HIF-1α as well as the regulation of Poldip2 expression at different oxygen concentrations – a task easily achievable thanks to the Hypoxystation’s intuitive interface for effortlessly adjusting the gas composition.

The Whitley  Hypoxystation provides the user with incredible flexibility as it can control oxygen down to 0.1% while providing a temperature and humidity controlled environment with ample working space. The atmosphere is constantly monitored and adjusted by the real-time feedback system to ensure accuracy.

 

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Supporting the Scientific Community

As well as manufacturing and supplying equipment for microbiology and cell culture applications worldwide, Don Whitley Scientific also makes an extensive effort to support the scientific community. For several years we have supported science through various means, these are listed below.

 

 

 

Workstation Poster Grant

If you have used a Whitley Workstation or Hypoxystation in your work and have mentioned it in your poster or published paper, let us know and you could be entitled to a grant of £250. All we ask in return is a copy of your poster/paper so we can use it on our website to help promote the range to others. Payment will be made in good faith after receipt and approval of the draft poster/paper to enable you to travel to the event to present it.

To apply for this grant, please send a copy of your draft poster or paper along with your contact details to steve_robertson@dwscientific.co.uk. If you are presenting a poster at an event, don’t forget to let us know the title of the event and the date you will be making your presentation.

 

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Picture of Helen Inns in The Mould That Changed the World

The Mould That Changed the World

Don Whitley Scientific has some very talented customers, not least Leeds Biomedical Scientist, Helen Inns. Helen is usually to be found at the WASPLab, working with bacteria including MRSA and Carbapenemase-Producing Enterobacteriaceae. This month, instead of just looking for “Superbugs”, she’s making a song and dance about them – at the Edinburgh Fringe in a show called The Mould That Changed The World.

Helen is part of a chorus of healthcare professionals performing in the world premiere of a brand-new musical, called The Mould That Changed the World. The show is a fun educational production which tells the story of Fleming’s discovery of penicillin, the development of antibiotics, and the looming crisis of antimicrobial resistance which now threatens healthcare as we know it.

Banner ad for The Mould that Changed the World

The Mould That Changed the World is receiving rave reviews at the Edinburgh Fringe, where it’s showing daily until 25th August.

After that, a free resource pack will be available, so primary schools can put on their own production and empower children to learn about and spread the message of antimicrobial stewardship.

Follow on Facebook and Twitter:  https://www.facebook.com/mouldchangedtheworld/

https://twitter.com/ThatMould

Find out more, or sign up for the schools production, at www.mouldthatchangedtheworld.com

 

 

wasp_lab_to be at Microbe 2018

Visit Don Whitley Scientific at Microbe 2018 and win a prize

 

Are you booked to attend this year’s Microbe in Sheffield? If you are, call at the Don Whitley Scientific stand to see WASPLab, ChromaZona and Whitley Workstations. There are also prizes to be won!

 

WASPLab

At what promises to be another entertaining, interesting, informative event, Don Whitley Scientific will showcase WASPLab. This is the complete robotic specimen processing system – including versatile software that conducts multiple plate scans during incubation, enabling early detection of positive growth. It provides the ability to perform antibiotic sensitivity testing; sub-culture work; and links to the MALDI-TOF identification system.

CHROMAZONA Automatic Colony Counter


ChromaZona

We will also be showing ChromaZona, the automated microbial identification and AST analysis system for faster results – and to EUCAST standards – in  busy clinical laboratories.

Whitley Workstations

Whitley A35 Anaerobic Workstation

Whitley A35 Workstation

If you need ideal conditions for the processing, incubation and examination of anaerobes and microaerophiles, come and discover the range of Whitley Workstations and the new Catalyst Monitoring System.

For more information

And if you are not able to attend Microbe this year but would like information on any of these products (or to discuss others in the Whitley range), please visit our website at www.dwscientific.co.uk or contact our sales office on sales@dwscientific.co.uk or call 01274 595728.

 

 

WASP_gram stain module

New Slide Preparation Module for WASPLab

DWS announces that from September 2018 a new Gram Slide Preparation Module for WASPLab™ will be available. This radically new slide prep module involves an exciting update to the technology, which significantly cuts maintenance costs. It also provides massive savings on consumables as, for example, it does not now require ink jet cartridges but uses laser technology to engrave the slides.

Automating slide selection and preparation in this way saves a great deal of time and prevents user error. With this new module, Copan once again prove how they continue to advance automation for the modern laboratory.

The previous Slide Preparation Module will be available for purchase only until February 2019, when it will be discontinued.

WASPLab™ is a sophisticated, barcode-driven microbiology specimen processor and work-up system. WASPLab™ moves samples from front end processing to full specimen management, with automated incubation and remote plate image analysis.

With its modular design and small footprint, WASPLab™ can be customised to the unique needs of your laboratory. The robotic plate management system, smart incubators and state-of-the-art image acquisition technology are changing the way labs work and are opening the door for ground-breaking digital microbiology. WASPLab™ is a truly modular system and can be added to when sample throughput increases, when additional resources are available and when new developments provide additional automated solutions.

 

 

 

 


Don Whitley Scientific have installed and maintained WASP and WASPLab systems in the UK for several years, sites include Manchester, Leeds and Blackburn.

For more information on WASPLab™, please don’t hesitate to get in touch via sales@dwscientific.co.uk or call us on +44 (0)1274 595728

 

PaCMAn Course Photo

Successful PaCMAn Course 2018

Another successful Practical and Clinical Microbiology of Anaerobes course was held on 14 & 15 June in Cardiff. We had a full complement of 26 delegates, including overseas visitors from Ireland, Norway, Sweden, Romania, Germany and Costa Rica.

This 2-day residential course enables delegates to refresh their knowledge on culturing, identification and the clinical importance of anaerobes and allows them to learn from clinical and laboratory professionals about the latest technologies and techniques. The course is delivered by the UK Anaerobe Reference Unit, Public Health Wales, Cardiff and is CPD accreditated by RCPath (11 credits).

 

The main aims of the course are:

·         To promote an understanding and awareness of anaerobic bacteria in clinical material.

·         To gain an insight into their relevance in clinical microbiology.

·         To achieve a basic level of competence in methods used for their isolation and identification.

·         To improve the standards of anaerobic microbiology in clinical laboratories.

 

Feedback from delegates has again been very positive with the majority rating it ‘excellent’ overall. Participants particularly enjoyed the friendly and informative course atmosphere and provided comments such as:

“Excellent course will definitely recommend to others.”

“Will be recommending for colleagues to attend.”

“Engaging speakers, very helpful, great team. I would highly recommend this course. I have learnt so much and look forward to passing all this great information to my team at work.”

“A fantastic course that came highly recommended to me and I will certainly
recommend to others.”

“It is an excellent workshop and I will recommend the course, even if you are working outside of the UK. Lectures were clear and well organized. The booklets are very educational for my needs.”

 

Whitley A55 WorkstationDon Whitley Scientific sponsors and helps to administer the course on behalf of the UK Anaerobe Reference Unit (ARU). We loaned the ARU a Whitley A55 Workstation for the course so that they had increased incubation capacity for the practical sessions.

It is planned to hold the course again in 2019 but dates have not yet been finalised. Keep an eye on the DWS blog or contact us on sales@dwscientific.co.uk if you are interested in attending this event.

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More Milestones Reached at Victoria Works

Don Whitley Scientific recently moved into new premises in Bingley, West Yorkshire. As expected, the move was completed in an efficient manner that has enabled the majority of our work to continue without interruption.

 

 

 

 

First workstations to leave VW

The first example of this is our first set of Whitley Workstations have been manufactured, tested and dispatched from our new workplace. On Monday, 2nd July, these first cabinets manufactured at our new premises went on final test. Only two weeks after moving to Bingley, these three DG250s are almost ready to be shipped to customers: two to Hua Yue in China and one to The University of Leeds. As mentioned this required a great deal of hard work from all involved to make sure Victoria Works was ready in time to get products out the door so quickly.

 

 

 

 

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A short while after most people started working at Victoria Works, it was time to get the specialist hauliers in to move the CNC machine shop department to Bingley. This was a large scale operation given the size and scale of the machines used by the department. However the move was managed well and they are now up and running in their new area, which offers a lot more space compared to what was available in Shipley.

 

 

 

 

 

Don Whitley Scientific has already welcomed various visitors to the new premises, where a new showroom is used to provide visitors with the experience of using DWS products first-hand. Tours of the premises are provided to give visitors a chance to see how the different departments work together to design, manufacture and service a comprehensive product range.

 

 

 

 

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Don Whitley Scientific opens new doors to visitors

With Don Whitley Scientific now firmly settled in its new home, Victoria Works, Bingley, visitors are now invited to a new showroom. This fabulous area boasts a permanent display of a wide range of Whitley products. Recently, a visitor from an increasingly relevant research topic in microbiology was the first visitor to the new premises.

Over the years, DWS invited many visitors to the old premises in nearby Shipley, from where the business ran successfully for over 30 years. However there were limitations on what products could be demonstrated due to the size of the old showroom. At Victoria Works the showroom is the ideal place for customers to come and really experience our range of workstations. You can try out some of the different porthole systems to see which would be the most suitable for your laboratory or look at the different options for size of airlock. We can sit down with you and discuss your application, your projects, what equipment you might want to use inside the chamber, the challenges you face, and what you need to achieve from the purchase of a Whitley Workstation.

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The Whitley i2 Instrument Workstation – Designed to accommodate Seahorse Extracellular Flux (XF) Analyzers in relevant conditions

Having a laboratory at Bingley provides additional benefits in that we have both microbiology and tissue culture experts on hand to give expert advice if and when required. We also have people who design and manufacture our workstations so if there is a slight modification or additional technical information required, we can get our R&D staff involved to discuss the possibilities.

 

 

The larger showroom now provides the option for bespoke areas to be created that reflect how different products may be used together in a lab environment. For example, sample preparation equipment (Smasher and Dilumat), spiral platers (Whitley WASP Touch) and colony counters (Protocol3). The size of the showroom also provides the option to display previous incarnations of DWS products, showcasing the evolution of products made possible through the work of our research and development team. For example you can see the evolution of Whitley Spiral Platers, with spiral plating being a process DWS helped establish and introduce to the world of microbiology.

 

 

 

 

 

 

Another way to view products is also provided by our 65″ screen. This offers the facility to showcase products, options and features with accompanying information and videos.

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The Whitley Interactive Product Presentation


If you are considering a Don Whitley Scientific product for your work, please don’t hesitate to get in touch to arrange a visit. We can tailor-make the visit based on your interests and ensure you are provided with understanding, knowledge and experience of DWS products. We can also give you a tour of the premises where you can see how all the different departments combine to create the products in the Don Whitley Scientific range.

Email sales@dwscientific.co.uk or give us a call on +44 (0)1274 595728


 

hepa filter

HEPA Filtration from Don Whitley Scientific

For over forty years, Don Whitley Scientific have utilised experience and expertise to ensure products make working methods more straight forward for our laboratory customers. With more and more importance placed on clean air, HEPA filtration can provide an extra measure to ensure research carried out within Whitley Workstations is done so in an environment that achieves particulate concentrations exceeding the levels detailed in ISO 14644-1 Part 1, Table 1, (Class 3) Classification of Air Cleanliness.

 

Tests have been carried out by Don Whitley Scientific that show just how much of a difference a HEPA filtration system makes to the atmosphere of a Whitley Workstation. Don Whitley Scientific have been manufacturing products for over 40 years and the introduction of HEPA filtration was a logical step to make sure our customers have an option that falls in line with the latest laboratory trends and requirements.

The benefits of the Whitley HEPA filtration system are:

  • The Whitley system utilises an internal HEPA filter. The atmosphere is cleaned quickly and continuously because the entire internal atmospheric volume of every Whitley Workstation passes through the filter hundreds of times an hour.
  • The atmosphere passing through the filter creates a laminar flow-like effect covering around 94% of the width of the chamber.
  • The location of the Whitley HEPA filter inside the workstation ensures it cannot get wet. Therefore it cannot become saturated with moisture and rendered ineffective.

Don Whitley Scientific has worked with one of the world’s leading filter manufacturers to develop a filter specifically tailored to suit the requirements of anaerobic workstations. Adding HEPA filter technology to the workstation means creating an environment where microbiology samples and cellular cultures can grow free from the risk of contamination.

HEPA filtration was introduced as an option after decades of experience in microbiology. We are confident that our thorough approach to product development and product testing has resulted in a HEPA filtration system superior to any other systems fitted to positive pressure modified atmosphere workstations. The video below explains Whitley HEPA filtration in further detail.

 

 


Explore the Whitley HEPA Workstation range